Larvae of Xenopus laevis at stage 46 or 50 were lentectomized and then fixed at various time intervals after the operation. Serial sections through the eye region were stained with hematoxylin and eosin or by the Himes Moriber technique to follow the histological changes taking place and to demonstrate any alterations in the basement membrane of the outer cornea. Lenses were observed to regenerate from the outer cornea while the basement membrane contributed to the capsule of the developing lens. A small lens with primary and secondary fibers was usually present by ten days after lentectomy. Corneas of Xenopus laevis larvae were implanted into lentectomized eyes, dorsal fins, amputated hindlimbs or subcutaneously in the head region. Lenses formed from the implants in the lentectomized eyes and in the amputated hindlimbs, but no lenses developed in the dorsal fins or from the subcutaneous implants.
ASJC Scopus subject areas
- Animal Science and Zoology