Lens connexins α3Cx46 and α8Cx50 interact with zonula occludens protein-1 (ZO-1)

Peter A. Nielsen, Amos Baruch, Valery I Shestopalov, Ben N G Giepmans, Irene Dunia, E. Lucio Benedetti, Nalin M. Kumar

Research output: Contribution to journalArticle

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Abstract

Connexin α1Cx43 has previously been shown to bind to the PDZ domain-containing protein ZO-1. The similarity of the carboxyl termini of this connexin and the lens fiber connexins α3Cx46 and α8Cx50 suggested that these connexins may also interact with ZO-1. ZO-1 was shown to be highly expressed in mouse lenses. Colocalization of ZO-1 with α3Cx46 and α8Cx50 connexins in fiber cells was demonstrated by immunofluorescence and by fracture-labeling electron microscopy but showed regional variations throughout the lens. ZO-1 was found to coimmunoprecipitate with α3Cx46 and α8Cx50, and pull-down experiments showed that the second PDZ domain of ZO-1 was involved in this interaction. Transiently expressed α3Cx46 and α8Cx50 connexins lacking the COOH-terminal residues did not bind to the second PDZ domain but still formed structures resembling gap junctions by immunofluorescence. These results indicate that ZO-1 interacts with lens fiber connexins α3Cx46 and α8Cx50 in a manner similar to that previously described for α1Cx43. The spatial variation in the interaction of ZO-1 with lens gap junctions is intriguing and is suggestive of multiple dynamic roles for this association.

Original languageEnglish
Pages (from-to)2470-2481
Number of pages12
JournalMolecular Biology of the Cell
Volume14
Issue number6
DOIs
StatePublished - Jun 1 2003

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Zonula Occludens-1 Protein
Connexins
Lenses
PDZ Domains
Gap Junctions
Fluorescent Antibody Technique
Electron Microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Nielsen, P. A., Baruch, A., Shestopalov, V. I., Giepmans, B. N. G., Dunia, I., Benedetti, E. L., & Kumar, N. M. (2003). Lens connexins α3Cx46 and α8Cx50 interact with zonula occludens protein-1 (ZO-1). Molecular Biology of the Cell, 14(6), 2470-2481. https://doi.org/10.1091/mbc.E02-10-0637

Lens connexins α3Cx46 and α8Cx50 interact with zonula occludens protein-1 (ZO-1). / Nielsen, Peter A.; Baruch, Amos; Shestopalov, Valery I; Giepmans, Ben N G; Dunia, Irene; Benedetti, E. Lucio; Kumar, Nalin M.

In: Molecular Biology of the Cell, Vol. 14, No. 6, 01.06.2003, p. 2470-2481.

Research output: Contribution to journalArticle

Nielsen, PA, Baruch, A, Shestopalov, VI, Giepmans, BNG, Dunia, I, Benedetti, EL & Kumar, NM 2003, 'Lens connexins α3Cx46 and α8Cx50 interact with zonula occludens protein-1 (ZO-1)', Molecular Biology of the Cell, vol. 14, no. 6, pp. 2470-2481. https://doi.org/10.1091/mbc.E02-10-0637
Nielsen, Peter A. ; Baruch, Amos ; Shestopalov, Valery I ; Giepmans, Ben N G ; Dunia, Irene ; Benedetti, E. Lucio ; Kumar, Nalin M. / Lens connexins α3Cx46 and α8Cx50 interact with zonula occludens protein-1 (ZO-1). In: Molecular Biology of the Cell. 2003 ; Vol. 14, No. 6. pp. 2470-2481.
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