Legionella pneumophila lipopolysaccharide activates the classical complement pathway

C. S. Mintz, D. R. Schultz, P. I. Arnold, W. Johnson

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Legionella pneumophila is a gram-negative bacterium capable of entering and growing in alveolar macrophages and monocytes. Complement and complement receptors are important in the uptake of L. pneumophila by human mononuclear phagocytes. The surface molecules of L. pneumophila that activate the complement system are unknown. To identify these factors, we investigated the effects of L. pneumophila lipopolysaccharide (LPS) on the classical and alternative complement pathways of normal human serum by functional hemolytic assays. Although incubation of LPS in normal human serum at 37°C resulted in the activation of both pathways, complement activation proceeded primarily through the classical pathway. Activation of the classical pathway by LPS was dependent on natural antibodies of the immunoglobulin M class that were present in various quantities in sera from different normal individuals but were absent in an immunoglobulin-deficient serum obtained from an agammaglobulinemic patient. Additional studies using sheep erythrocytes coated with LPS suggested that the antibodies recognized antigenic sites in the carbohydrate portion of LPS. The ability of LPS to interact with the complement system suggests a role for LPS in the uptake of L. pneumophila by mononuclear phagocytes.

Original languageEnglish
Pages (from-to)2769-2776
Number of pages8
JournalInfection and Immunity
Volume60
Issue number7
StatePublished - Jan 1 1992
Externally publishedYes

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Classical Complement Pathway
Legionella pneumophila
Lipopolysaccharides
Phagocytes
Serum
Alternative Complement Pathway
Complement Receptors
Antibodies
Immunoglobulin Isotypes
Complement Activation
Alveolar Macrophages
Gram-Negative Bacteria
Immunoglobulin M
Immunoglobulins
Monocytes
Sheep
Erythrocytes
Carbohydrates

ASJC Scopus subject areas

  • Immunology

Cite this

Mintz, C. S., Schultz, D. R., Arnold, P. I., & Johnson, W. (1992). Legionella pneumophila lipopolysaccharide activates the classical complement pathway. Infection and Immunity, 60(7), 2769-2776.

Legionella pneumophila lipopolysaccharide activates the classical complement pathway. / Mintz, C. S.; Schultz, D. R.; Arnold, P. I.; Johnson, W.

In: Infection and Immunity, Vol. 60, No. 7, 01.01.1992, p. 2769-2776.

Research output: Contribution to journalArticle

Mintz, CS, Schultz, DR, Arnold, PI & Johnson, W 1992, 'Legionella pneumophila lipopolysaccharide activates the classical complement pathway', Infection and Immunity, vol. 60, no. 7, pp. 2769-2776.
Mintz CS, Schultz DR, Arnold PI, Johnson W. Legionella pneumophila lipopolysaccharide activates the classical complement pathway. Infection and Immunity. 1992 Jan 1;60(7):2769-2776.
Mintz, C. S. ; Schultz, D. R. ; Arnold, P. I. ; Johnson, W. / Legionella pneumophila lipopolysaccharide activates the classical complement pathway. In: Infection and Immunity. 1992 ; Vol. 60, No. 7. pp. 2769-2776.
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