Lectin-detectable effects of localized pneumonia on airway mucous cell populations

Role of cyclooxygenase metabolites

Andrew T. Mariassy, Micheal N. McCray, Isabel T. Lauredo, William M. Abraham, Adam Wanner

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We examined the airway secretory apparatus of adult sheep with experimental pneumonia to look for morphologic and lectin-binding correlates of increased mucus production. The animals were inoculated in the right caudal lobar bronchus either with starch broth containing Pasteurella haemolytica (INF, n = 6), starch broth alone (SHAM, n = 6), or with P. haemolytica and subsequently treated (INF/T, n = 5) with 2 mg/kg indomethacin, subcutaneously three times daily for 6 days. In the INF and INF/T groups, a localized pneumonic infiltrate containing P. haemolytica organisms was present. The bronchi (18-23rd generation) adjacent to the pneumonic lesion had an increased gland volume fraction (6.3 ' 3.7% in INF, 11.3 ' 2.4% in INF/T, and 3.1 ' 1.9% in SHAM, p < 0.05 among the three). The mean population densities of BSA-reactive (identifying αV-gal) cells were 41.9 ' 2.7% in the INF, 40.1 ' 5.6% in the INF/T, versus 14.3 ' 1.5% in the SHAM group (p < 0.05), while the corresponding values for PNA-reactive [identifying βD-gal(1→ 3)-D-galNAc] cells were 28.8 ' 5.1% 0% and 0% respectively. Nor morphologic abnormalities were seen in the trachea, but BSA staining was shifted to morphologically different mucous cells in the INF and INF/T. We conclude that in localized P. haemolytica pneumonia in sheep (1) there are morphologic changes of the airway secretory apparatus adjacent to the lesion, (2) the glycoconjugate profile of secretory cells adjacent to and remote from the lesion is altered, and (3) cyclooxygenase products influence the chemical composition of secretory cells.

Original languageEnglish
Pages (from-to)113-137
Number of pages25
JournalExperimental Lung Research
Volume15
Issue number1
DOIs
StatePublished - Jan 1 1989

Fingerprint

Mannheimia haemolytica
Prostaglandin-Endoperoxide Synthases
Metabolites
Lectins
Pneumonia
Cells
Starch
Population
Bronchi
Glycoconjugates
Sheep
Indomethacin
Volume fraction
Animals
Lung
Mucus
Population Density
Trachea
Chemical analysis
salicylhydroxamic acid

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Lectin-detectable effects of localized pneumonia on airway mucous cell populations : Role of cyclooxygenase metabolites. / Mariassy, Andrew T.; McCray, Micheal N.; Lauredo, Isabel T.; Abraham, William M.; Wanner, Adam.

In: Experimental Lung Research, Vol. 15, No. 1, 01.01.1989, p. 113-137.

Research output: Contribution to journalArticle

Mariassy, Andrew T. ; McCray, Micheal N. ; Lauredo, Isabel T. ; Abraham, William M. ; Wanner, Adam. / Lectin-detectable effects of localized pneumonia on airway mucous cell populations : Role of cyclooxygenase metabolites. In: Experimental Lung Research. 1989 ; Vol. 15, No. 1. pp. 113-137.
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abstract = "We examined the airway secretory apparatus of adult sheep with experimental pneumonia to look for morphologic and lectin-binding correlates of increased mucus production. The animals were inoculated in the right caudal lobar bronchus either with starch broth containing Pasteurella haemolytica (INF, n = 6), starch broth alone (SHAM, n = 6), or with P. haemolytica and subsequently treated (INF/T, n = 5) with 2 mg/kg indomethacin, subcutaneously three times daily for 6 days. In the INF and INF/T groups, a localized pneumonic infiltrate containing P. haemolytica organisms was present. The bronchi (18-23rd generation) adjacent to the pneumonic lesion had an increased gland volume fraction (6.3 ' 3.7{\%} in INF, 11.3 ' 2.4{\%} in INF/T, and 3.1 ' 1.9{\%} in SHAM, p < 0.05 among the three). The mean population densities of BSA-reactive (identifying αV-gal) cells were 41.9 ' 2.7{\%} in the INF, 40.1 ' 5.6{\%} in the INF/T, versus 14.3 ' 1.5{\%} in the SHAM group (p < 0.05), while the corresponding values for PNA-reactive [identifying βD-gal(1→ 3)-D-galNAc] cells were 28.8 ' 5.1{\%} 0{\%} and 0{\%} respectively. Nor morphologic abnormalities were seen in the trachea, but BSA staining was shifted to morphologically different mucous cells in the INF and INF/T. We conclude that in localized P. haemolytica pneumonia in sheep (1) there are morphologic changes of the airway secretory apparatus adjacent to the lesion, (2) the glycoconjugate profile of secretory cells adjacent to and remote from the lesion is altered, and (3) cyclooxygenase products influence the chemical composition of secretory cells.",
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