LEA.135 expression

Its comparison with other prognostic biomarkers for patients with primary breast carcinoma

Dongxin Liu, Armine Baltayan, Wesley Y. Naritoku, Nancy J. Barr, Lillian L. Young, Benjaporn Chaiwun, Denice D. Tsao-Wei, Susan L. Groshen, Clive R. Taylor, Humberto Torloni, A. Munro Neville, Richard J Cote, S. Ashraf Imam

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The purpose of this retrospective study was to examine the prognostic value of expression of luminal epithelial antigen (LEA.135) for recurrence and overall survival of patients with primary invasive breast carcinoma by both univariate and multivariate analyses. The possible prognostic value of LEA.135 was also compared with some widely utilized prognostic biomarkers such as c-erbB 2, topoisomerase II·α (TPII·α), MIB 1, estrogen receptor (ER) and progesterone receptor (PR), as well as age of the patients and clinicopathologic parameters. The study was carried out by immunohistochemical methods on formalin-fixed/paraffin-embedded tissue sections in a series of 225 patients with median follow-up of 8.5 years. Prognostic significance of the biomarkers was determined by two-sided p value. In this series of patients, among the age and clinicopathologic parameters, only age, was significantly associated with a decreased overall survival (logrank p = 0.027). Among the prognostic biomarkers, TPII a expression at high (> 50% positive cells) or moderate (6-50% positive cells) level was associated with an increased rate of recurrence (logrank p < 0.001). However, the association of TPII·α expression with a decreased overall survival failed to reach a statistically significance. Expression of c-erbB 2 showed a trend of being associated with an increased probability of recurrence but the association did not reach statistical significance. The remaining biomarkers were not associated with either the probability of recurrence or overall survival LEA.135 expression was observed in 163 (72.4%) of the 225 patients. The patients with high (> 50% positive cells) or moderate (6-50% positive cells) level of LEA.135-positive cancer cells showed a significantly decreased probability of recurrence (logrank p < 0.001) and an increased overall survival (logrank p < 0.001) compared with those with LEA.135-negative cancer cells. The association remained significant by multivariate analysis for recurrence (likelihood ratio test p < 0.001) and overall survival (likelihood ratio test p < 0.001) when assessed with other prognostic parameters. Furthermore, the combination of LEA.135 with other prognostic biomarkers stratified four subgroups of patients with distinct clinical oulcome. The subgroup of patients who were LEA.135+/TPII·α- showed the lowest probability of recurrence and the longest overall survival compared with those who were LEA.135-/TPII·α+ (logrank p < 0.001). Interestingly, the patients whose cancer cells were LEA.135+/TPII·α+, LEA.135+ MIB·1+ or LEA.135+/c-erbB2+ experienced a decreased probability of recurrence and an increased overall survival compared with those with LEA.135-/TPII·α+, LEA.135-MIB·1+ or LEA.135-/c-erbB2+ (logrank p < 0.001). The results demonstrated that LEA.135 is an independent and favorable prognostic biomarker for patients with primary invasive breast carcinoma, that the loss of LEA.135 expression is associated with aggressive phenotype of cancer cells during the breast cancer progression, and that its continued expression seems to override the adverse effects of expression of an oncogene or cell proliferation-associated molecules.

Original languageEnglish
Pages (from-to)1451-1461
Number of pages11
JournalAnticancer Research
Volume20
Issue number3 A
StatePublished - Aug 30 2000
Externally publishedYes

Fingerprint

Type II DNA Topoisomerase
Biomarkers
Breast Neoplasms
Recurrence
Survival
Neoplasms
Multivariate Analysis
Progesterone Receptors
Oncogenes
Estrogen Receptors
Paraffin
Formaldehyde
Retrospective Studies
Cell Proliferation
Phenotype
Antigens

Keywords

  • Breast carcinoma
  • c-erbB-2
  • Estrogen receptor
  • LEA. 135
  • MIB-1
  • Progesterone receptor
  • Prognosis
  • Topoisomerase II·α

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Liu, D., Baltayan, A., Naritoku, W. Y., Barr, N. J., Young, L. L., Chaiwun, B., ... Imam, S. A. (2000). LEA.135 expression: Its comparison with other prognostic biomarkers for patients with primary breast carcinoma. Anticancer Research, 20(3 A), 1451-1461.

LEA.135 expression : Its comparison with other prognostic biomarkers for patients with primary breast carcinoma. / Liu, Dongxin; Baltayan, Armine; Naritoku, Wesley Y.; Barr, Nancy J.; Young, Lillian L.; Chaiwun, Benjaporn; Tsao-Wei, Denice D.; Groshen, Susan L.; Taylor, Clive R.; Torloni, Humberto; Neville, A. Munro; Cote, Richard J; Imam, S. Ashraf.

In: Anticancer Research, Vol. 20, No. 3 A, 30.08.2000, p. 1451-1461.

Research output: Contribution to journalArticle

Liu, D, Baltayan, A, Naritoku, WY, Barr, NJ, Young, LL, Chaiwun, B, Tsao-Wei, DD, Groshen, SL, Taylor, CR, Torloni, H, Neville, AM, Cote, RJ & Imam, SA 2000, 'LEA.135 expression: Its comparison with other prognostic biomarkers for patients with primary breast carcinoma', Anticancer Research, vol. 20, no. 3 A, pp. 1451-1461.
Liu D, Baltayan A, Naritoku WY, Barr NJ, Young LL, Chaiwun B et al. LEA.135 expression: Its comparison with other prognostic biomarkers for patients with primary breast carcinoma. Anticancer Research. 2000 Aug 30;20(3 A):1451-1461.
Liu, Dongxin ; Baltayan, Armine ; Naritoku, Wesley Y. ; Barr, Nancy J. ; Young, Lillian L. ; Chaiwun, Benjaporn ; Tsao-Wei, Denice D. ; Groshen, Susan L. ; Taylor, Clive R. ; Torloni, Humberto ; Neville, A. Munro ; Cote, Richard J ; Imam, S. Ashraf. / LEA.135 expression : Its comparison with other prognostic biomarkers for patients with primary breast carcinoma. In: Anticancer Research. 2000 ; Vol. 20, No. 3 A. pp. 1451-1461.
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title = "LEA.135 expression: Its comparison with other prognostic biomarkers for patients with primary breast carcinoma",
abstract = "The purpose of this retrospective study was to examine the prognostic value of expression of luminal epithelial antigen (LEA.135) for recurrence and overall survival of patients with primary invasive breast carcinoma by both univariate and multivariate analyses. The possible prognostic value of LEA.135 was also compared with some widely utilized prognostic biomarkers such as c-erbB 2, topoisomerase II·α (TPII·α), MIB 1, estrogen receptor (ER) and progesterone receptor (PR), as well as age of the patients and clinicopathologic parameters. The study was carried out by immunohistochemical methods on formalin-fixed/paraffin-embedded tissue sections in a series of 225 patients with median follow-up of 8.5 years. Prognostic significance of the biomarkers was determined by two-sided p value. In this series of patients, among the age and clinicopathologic parameters, only age, was significantly associated with a decreased overall survival (logrank p = 0.027). Among the prognostic biomarkers, TPII a expression at high (> 50{\%} positive cells) or moderate (6-50{\%} positive cells) level was associated with an increased rate of recurrence (logrank p < 0.001). However, the association of TPII·α expression with a decreased overall survival failed to reach a statistically significance. Expression of c-erbB 2 showed a trend of being associated with an increased probability of recurrence but the association did not reach statistical significance. The remaining biomarkers were not associated with either the probability of recurrence or overall survival LEA.135 expression was observed in 163 (72.4{\%}) of the 225 patients. The patients with high (> 50{\%} positive cells) or moderate (6-50{\%} positive cells) level of LEA.135-positive cancer cells showed a significantly decreased probability of recurrence (logrank p < 0.001) and an increased overall survival (logrank p < 0.001) compared with those with LEA.135-negative cancer cells. The association remained significant by multivariate analysis for recurrence (likelihood ratio test p < 0.001) and overall survival (likelihood ratio test p < 0.001) when assessed with other prognostic parameters. Furthermore, the combination of LEA.135 with other prognostic biomarkers stratified four subgroups of patients with distinct clinical oulcome. The subgroup of patients who were LEA.135+/TPII·α- showed the lowest probability of recurrence and the longest overall survival compared with those who were LEA.135-/TPII·α+ (logrank p < 0.001). Interestingly, the patients whose cancer cells were LEA.135+/TPII·α+, LEA.135+ MIB·1+ or LEA.135+/c-erbB2+ experienced a decreased probability of recurrence and an increased overall survival compared with those with LEA.135-/TPII·α+, LEA.135-MIB·1+ or LEA.135-/c-erbB2+ (logrank p < 0.001). The results demonstrated that LEA.135 is an independent and favorable prognostic biomarker for patients with primary invasive breast carcinoma, that the loss of LEA.135 expression is associated with aggressive phenotype of cancer cells during the breast cancer progression, and that its continued expression seems to override the adverse effects of expression of an oncogene or cell proliferation-associated molecules.",
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author = "Dongxin Liu and Armine Baltayan and Naritoku, {Wesley Y.} and Barr, {Nancy J.} and Young, {Lillian L.} and Benjaporn Chaiwun and Tsao-Wei, {Denice D.} and Groshen, {Susan L.} and Taylor, {Clive R.} and Humberto Torloni and Neville, {A. Munro} and Cote, {Richard J} and Imam, {S. Ashraf}",
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TY - JOUR

T1 - LEA.135 expression

T2 - Its comparison with other prognostic biomarkers for patients with primary breast carcinoma

AU - Liu, Dongxin

AU - Baltayan, Armine

AU - Naritoku, Wesley Y.

AU - Barr, Nancy J.

AU - Young, Lillian L.

AU - Chaiwun, Benjaporn

AU - Tsao-Wei, Denice D.

AU - Groshen, Susan L.

AU - Taylor, Clive R.

AU - Torloni, Humberto

AU - Neville, A. Munro

AU - Cote, Richard J

AU - Imam, S. Ashraf

PY - 2000/8/30

Y1 - 2000/8/30

N2 - The purpose of this retrospective study was to examine the prognostic value of expression of luminal epithelial antigen (LEA.135) for recurrence and overall survival of patients with primary invasive breast carcinoma by both univariate and multivariate analyses. The possible prognostic value of LEA.135 was also compared with some widely utilized prognostic biomarkers such as c-erbB 2, topoisomerase II·α (TPII·α), MIB 1, estrogen receptor (ER) and progesterone receptor (PR), as well as age of the patients and clinicopathologic parameters. The study was carried out by immunohistochemical methods on formalin-fixed/paraffin-embedded tissue sections in a series of 225 patients with median follow-up of 8.5 years. Prognostic significance of the biomarkers was determined by two-sided p value. In this series of patients, among the age and clinicopathologic parameters, only age, was significantly associated with a decreased overall survival (logrank p = 0.027). Among the prognostic biomarkers, TPII a expression at high (> 50% positive cells) or moderate (6-50% positive cells) level was associated with an increased rate of recurrence (logrank p < 0.001). However, the association of TPII·α expression with a decreased overall survival failed to reach a statistically significance. Expression of c-erbB 2 showed a trend of being associated with an increased probability of recurrence but the association did not reach statistical significance. The remaining biomarkers were not associated with either the probability of recurrence or overall survival LEA.135 expression was observed in 163 (72.4%) of the 225 patients. The patients with high (> 50% positive cells) or moderate (6-50% positive cells) level of LEA.135-positive cancer cells showed a significantly decreased probability of recurrence (logrank p < 0.001) and an increased overall survival (logrank p < 0.001) compared with those with LEA.135-negative cancer cells. The association remained significant by multivariate analysis for recurrence (likelihood ratio test p < 0.001) and overall survival (likelihood ratio test p < 0.001) when assessed with other prognostic parameters. Furthermore, the combination of LEA.135 with other prognostic biomarkers stratified four subgroups of patients with distinct clinical oulcome. The subgroup of patients who were LEA.135+/TPII·α- showed the lowest probability of recurrence and the longest overall survival compared with those who were LEA.135-/TPII·α+ (logrank p < 0.001). Interestingly, the patients whose cancer cells were LEA.135+/TPII·α+, LEA.135+ MIB·1+ or LEA.135+/c-erbB2+ experienced a decreased probability of recurrence and an increased overall survival compared with those with LEA.135-/TPII·α+, LEA.135-MIB·1+ or LEA.135-/c-erbB2+ (logrank p < 0.001). The results demonstrated that LEA.135 is an independent and favorable prognostic biomarker for patients with primary invasive breast carcinoma, that the loss of LEA.135 expression is associated with aggressive phenotype of cancer cells during the breast cancer progression, and that its continued expression seems to override the adverse effects of expression of an oncogene or cell proliferation-associated molecules.

AB - The purpose of this retrospective study was to examine the prognostic value of expression of luminal epithelial antigen (LEA.135) for recurrence and overall survival of patients with primary invasive breast carcinoma by both univariate and multivariate analyses. The possible prognostic value of LEA.135 was also compared with some widely utilized prognostic biomarkers such as c-erbB 2, topoisomerase II·α (TPII·α), MIB 1, estrogen receptor (ER) and progesterone receptor (PR), as well as age of the patients and clinicopathologic parameters. The study was carried out by immunohistochemical methods on formalin-fixed/paraffin-embedded tissue sections in a series of 225 patients with median follow-up of 8.5 years. Prognostic significance of the biomarkers was determined by two-sided p value. In this series of patients, among the age and clinicopathologic parameters, only age, was significantly associated with a decreased overall survival (logrank p = 0.027). Among the prognostic biomarkers, TPII a expression at high (> 50% positive cells) or moderate (6-50% positive cells) level was associated with an increased rate of recurrence (logrank p < 0.001). However, the association of TPII·α expression with a decreased overall survival failed to reach a statistically significance. Expression of c-erbB 2 showed a trend of being associated with an increased probability of recurrence but the association did not reach statistical significance. The remaining biomarkers were not associated with either the probability of recurrence or overall survival LEA.135 expression was observed in 163 (72.4%) of the 225 patients. The patients with high (> 50% positive cells) or moderate (6-50% positive cells) level of LEA.135-positive cancer cells showed a significantly decreased probability of recurrence (logrank p < 0.001) and an increased overall survival (logrank p < 0.001) compared with those with LEA.135-negative cancer cells. The association remained significant by multivariate analysis for recurrence (likelihood ratio test p < 0.001) and overall survival (likelihood ratio test p < 0.001) when assessed with other prognostic parameters. Furthermore, the combination of LEA.135 with other prognostic biomarkers stratified four subgroups of patients with distinct clinical oulcome. The subgroup of patients who were LEA.135+/TPII·α- showed the lowest probability of recurrence and the longest overall survival compared with those who were LEA.135-/TPII·α+ (logrank p < 0.001). Interestingly, the patients whose cancer cells were LEA.135+/TPII·α+, LEA.135+ MIB·1+ or LEA.135+/c-erbB2+ experienced a decreased probability of recurrence and an increased overall survival compared with those with LEA.135-/TPII·α+, LEA.135-MIB·1+ or LEA.135-/c-erbB2+ (logrank p < 0.001). The results demonstrated that LEA.135 is an independent and favorable prognostic biomarker for patients with primary invasive breast carcinoma, that the loss of LEA.135 expression is associated with aggressive phenotype of cancer cells during the breast cancer progression, and that its continued expression seems to override the adverse effects of expression of an oncogene or cell proliferation-associated molecules.

KW - Breast carcinoma

KW - c-erbB-2

KW - Estrogen receptor

KW - LEA. 135

KW - MIB-1

KW - Progesterone receptor

KW - Prognosis

KW - Topoisomerase II·α

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