LDH-B enzyme expression: The mechanisms of altered gene expression in acclimation and evolutionary adaptation

J. A. Segal, D. L. Crawford

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

The temperature-dependent expression of lactate dehydrogenase-B (LDH-B) was compared between two environmentally distinct populations of Fundulus heteroclitus acclimated to 10°C and 20°C. The variability in LDH-B protein expression both within and between populations is consistent with a model of thermal compensation. The northern population from the colder environment expresses a twofold greater amount of LDH-B protein than the warmer southern population at both acclimation temperatures. Correspondingly, both populations have 1.3-fold greater levels of the enzyme at an acclimation temperature of 10°C in comparison to 20°C. In 20°C-acclimated individuals there is a similar twofold difference between populations for LDH-B mRNA concentrations, and LDH-B protein and mRNA are highly correlated (r = 0.81). After acclimation to 10°C, this difference between populations is not seen and in the northern population there is no relationship between LDH-B mRNA and protein levels. Thus the molecular mechanism regulating LDH-B enzyme expression changes in response to temperature acclimation and is different between populations.

Original languageEnglish (US)
Pages (from-to)R1150-R1153
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume267
Issue number4 36-4
StatePublished - Jan 1 1994
Externally publishedYes

Keywords

  • enzyme concentration
  • Fundulus heteroclitus
  • lactate dehydrogenase
  • messenger ribonucleic acid

ASJC Scopus subject areas

  • Physiology

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