LcrD, a membrane-bound regulator of the Yersinia pestis low-calcium response

Gregory V Plano, S. S. Barve, S. C. Straley

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Yersinia pestis, the etiologic agent of bubonic plague, contains a 75-kb virulence plasmid, called pCD1 in Y. pestis KIM. The low-Ca2+-response genes of Y. pestis regulate both bacterial growth and the expression of pCD1-encoded virulence determinants in response to temperature and the presence of Ca2+ or nucleotides. This study characterizes the nucleotide sequence and protein product of the lcrD locus. An lcrD mutant, in contrast to the parent Y. pestis, did not undergo growth restriction or induce strong expression of the V antigen when grown under conditions (37°C, no Ca2+) expected to elicit maximal expression of pCD1 genes. DNA sequence analysis of the cloned lcrD locus showed a single open reading frame that could encode a protein with a molecular weight of 77,804 and a pI of 4.88. LcrD was identified as a 70-kDa inner membrane protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis. LcrD membrane topology was investigated by using lcrD-phoA translational fusions generated with the transposon TnphoA. The alkaline phosphatase activities of the resultant hybrid proteins were consistent with a model predicting eight amino-terminal transmembrane segments that anchor a large cytoplasmic carboxyl-terminal domain to the inner membrane.

Original languageEnglish
Pages (from-to)7293-7303
Number of pages11
JournalJournal of Bacteriology
Volume173
Issue number22
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Yersinia pestis
Calcium
Membranes
Virulence
Proteins
Plague
Growth
DNA Sequence Analysis
Sodium Dodecyl Sulfate
Open Reading Frames
Alkaline Phosphatase
Polyacrylamide Gel Electrophoresis
Membrane Proteins
Plasmids
Nucleotides
Molecular Weight
Gene Expression
Antigens
Temperature
Genes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

LcrD, a membrane-bound regulator of the Yersinia pestis low-calcium response. / Plano, Gregory V; Barve, S. S.; Straley, S. C.

In: Journal of Bacteriology, Vol. 173, No. 22, 01.01.1991, p. 7293-7303.

Research output: Contribution to journalArticle

Plano, Gregory V ; Barve, S. S. ; Straley, S. C. / LcrD, a membrane-bound regulator of the Yersinia pestis low-calcium response. In: Journal of Bacteriology. 1991 ; Vol. 173, No. 22. pp. 7293-7303.
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