Lattice defects in microtubules: Protofilament numbers vary within individual microtubules

D. Chretien, F. Metoz, F. Verde, E. Karsenti, R. H. Wade

Research output: Contribution to journalArticle

154 Scopus citations

Abstract

We have used cryo-electron microscopy of vitrified specimens to study microtubules assembled both from three cycle purified tubulin (3×-tubulin) and in cell free extracts of Xenopus eggs. In vitro assembled 3×-tubulin samples have a majority of microtubules with 14 protofilaments whereas in cell extracts most microtubules have 13 protofilaments. Microtubule polymorphism was observed in both cases. The number of protofilaments can change abruptly along individual microtubules usually by single increments but double increments also occur. For 3×-tubulin, increasing the magnesium concentration decreases the proportion of 14 protofilament microtubules and decreases the average separation between transitions in these microtubules. Protofilament discontinuities may correspond to dislocation-like defects in the microtubule surface lattice.

Original languageEnglish (US)
Pages (from-to)1031-1040
Number of pages10
JournalJournal of Cell Biology
Volume117
Issue number5
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Lattice defects in microtubules: Protofilament numbers vary within individual microtubules'. Together they form a unique fingerprint.

  • Cite this