Latex test for serodiagnosis of infectious mononucleosis

B. A. Levey, T. M. Lo, K. E. Caldwell, M. A. Fletcher

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A glycoprotein was isolated from bovine erythrocytes which has 20% carbohydrate and migrates on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single band. This glycoprotein carries the reactivity of bovine erythrocytes with Paul-Bunnell heterophile antibody of infectious mononucleosis. This bovine glycoprotein was coupled to carboxyl-modified latex particles with water-soluble carbodiimide. The resulting reagent was then used to develop a new test for the detection of infectious mononucleosis antibody. The bovine erythrocyte glycoprotein-latex reagent is more stable than sheep or horse erythrocytes, the traditional reagents for detection of infectious mononucleosis antibody. This new reagent is used in a direct slide test; no preabsorption of the sera is necessary. In the present study the glycoprotein-latex reagent compared favorably in terms of sensitivity and specificity with two standard tests for infectious mononculeosis antibody. Ninety-nine serum samples were tested. Agreement of the latex test with a stabilized horse erythrocyte spot test was 90%. Ten samples were weakly positive with the latex test and negative with the horse cell test. Only one of these was also positive with an enzyme-treated sheep cell test. This latter test was somewhat more sensitive than the latex test.

Original languageEnglish
Pages (from-to)256-262
Number of pages7
JournalJournal of Clinical Microbiology
Volume11
Issue number3
StatePublished - Jan 1 1980
Externally publishedYes

Fingerprint

Infectious Mononucleosis
Latex
Serologic Tests
Glycoproteins
Erythrocytes
Horses
Heterophile Antibodies
Antibodies
Sheep
Carbodiimides
Serum
Microspheres
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Carbohydrates
Sensitivity and Specificity
Water
Enzymes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Levey, B. A., Lo, T. M., Caldwell, K. E., & Fletcher, M. A. (1980). Latex test for serodiagnosis of infectious mononucleosis. Journal of Clinical Microbiology, 11(3), 256-262.

Latex test for serodiagnosis of infectious mononucleosis. / Levey, B. A.; Lo, T. M.; Caldwell, K. E.; Fletcher, M. A.

In: Journal of Clinical Microbiology, Vol. 11, No. 3, 01.01.1980, p. 256-262.

Research output: Contribution to journalArticle

Levey, BA, Lo, TM, Caldwell, KE & Fletcher, MA 1980, 'Latex test for serodiagnosis of infectious mononucleosis', Journal of Clinical Microbiology, vol. 11, no. 3, pp. 256-262.
Levey BA, Lo TM, Caldwell KE, Fletcher MA. Latex test for serodiagnosis of infectious mononucleosis. Journal of Clinical Microbiology. 1980 Jan 1;11(3):256-262.
Levey, B. A. ; Lo, T. M. ; Caldwell, K. E. ; Fletcher, M. A. / Latex test for serodiagnosis of infectious mononucleosis. In: Journal of Clinical Microbiology. 1980 ; Vol. 11, No. 3. pp. 256-262.
@article{141c1471196f412abf45568e2b5d9c15,
title = "Latex test for serodiagnosis of infectious mononucleosis",
abstract = "A glycoprotein was isolated from bovine erythrocytes which has 20{\%} carbohydrate and migrates on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single band. This glycoprotein carries the reactivity of bovine erythrocytes with Paul-Bunnell heterophile antibody of infectious mononucleosis. This bovine glycoprotein was coupled to carboxyl-modified latex particles with water-soluble carbodiimide. The resulting reagent was then used to develop a new test for the detection of infectious mononucleosis antibody. The bovine erythrocyte glycoprotein-latex reagent is more stable than sheep or horse erythrocytes, the traditional reagents for detection of infectious mononucleosis antibody. This new reagent is used in a direct slide test; no preabsorption of the sera is necessary. In the present study the glycoprotein-latex reagent compared favorably in terms of sensitivity and specificity with two standard tests for infectious mononculeosis antibody. Ninety-nine serum samples were tested. Agreement of the latex test with a stabilized horse erythrocyte spot test was 90{\%}. Ten samples were weakly positive with the latex test and negative with the horse cell test. Only one of these was also positive with an enzyme-treated sheep cell test. This latter test was somewhat more sensitive than the latex test.",
author = "Levey, {B. A.} and Lo, {T. M.} and Caldwell, {K. E.} and Fletcher, {M. A.}",
year = "1980",
month = "1",
day = "1",
language = "English",
volume = "11",
pages = "256--262",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Latex test for serodiagnosis of infectious mononucleosis

AU - Levey, B. A.

AU - Lo, T. M.

AU - Caldwell, K. E.

AU - Fletcher, M. A.

PY - 1980/1/1

Y1 - 1980/1/1

N2 - A glycoprotein was isolated from bovine erythrocytes which has 20% carbohydrate and migrates on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single band. This glycoprotein carries the reactivity of bovine erythrocytes with Paul-Bunnell heterophile antibody of infectious mononucleosis. This bovine glycoprotein was coupled to carboxyl-modified latex particles with water-soluble carbodiimide. The resulting reagent was then used to develop a new test for the detection of infectious mononucleosis antibody. The bovine erythrocyte glycoprotein-latex reagent is more stable than sheep or horse erythrocytes, the traditional reagents for detection of infectious mononucleosis antibody. This new reagent is used in a direct slide test; no preabsorption of the sera is necessary. In the present study the glycoprotein-latex reagent compared favorably in terms of sensitivity and specificity with two standard tests for infectious mononculeosis antibody. Ninety-nine serum samples were tested. Agreement of the latex test with a stabilized horse erythrocyte spot test was 90%. Ten samples were weakly positive with the latex test and negative with the horse cell test. Only one of these was also positive with an enzyme-treated sheep cell test. This latter test was somewhat more sensitive than the latex test.

AB - A glycoprotein was isolated from bovine erythrocytes which has 20% carbohydrate and migrates on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single band. This glycoprotein carries the reactivity of bovine erythrocytes with Paul-Bunnell heterophile antibody of infectious mononucleosis. This bovine glycoprotein was coupled to carboxyl-modified latex particles with water-soluble carbodiimide. The resulting reagent was then used to develop a new test for the detection of infectious mononucleosis antibody. The bovine erythrocyte glycoprotein-latex reagent is more stable than sheep or horse erythrocytes, the traditional reagents for detection of infectious mononucleosis antibody. This new reagent is used in a direct slide test; no preabsorption of the sera is necessary. In the present study the glycoprotein-latex reagent compared favorably in terms of sensitivity and specificity with two standard tests for infectious mononculeosis antibody. Ninety-nine serum samples were tested. Agreement of the latex test with a stabilized horse erythrocyte spot test was 90%. Ten samples were weakly positive with the latex test and negative with the horse cell test. Only one of these was also positive with an enzyme-treated sheep cell test. This latter test was somewhat more sensitive than the latex test.

UR - http://www.scopus.com/inward/record.url?scp=0018890734&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018890734&partnerID=8YFLogxK

M3 - Article

C2 - 6247367

AN - SCOPUS:0018890734

VL - 11

SP - 256

EP - 262

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 3

ER -