Latent Membrane Protein 1 as a molecular adjuvant for single-cycle lentiviral vaccines

Sachin Gupta, James M. Termini, Liguo Niu, Saravana K. Kanagavelu, Andrew R. Rahmberg, Richard S. Kornbluth, David T. Evans, Geoffrey Stone

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Molecular adjuvants are a promising method to enhance virus-specific immune responses and protect against HIV-1 infection. Immune activation by ligands for receptors such as CD40 can induce dendritic cell activation and maturation. Here we explore the incorporation of two CD40 mimics, Epstein Barr Virus gene LMP1 or an LMP1-CD40 chimera, into a strain of SIV that was engineered to be limited to a single cycle of infection.Results: Full length LMP1 or the chimeric protein LMP1-CD40 was cloned into the nef-locus of single-cycle SIV. Human and Macaque monocyte derived macrophages and DC were infected with these viruses. Infected cells were analyzed for activation surface markers by flow cytometry. Cells were also analyzed for secretion of pro-inflammatory cytokines IL-1β, IL-6, IL-8, IL-12p70 and TNF by cytometric bead array.Conclusions: Overall, single-cycle SIV expressing LMP1 and LMP1-CD40 produced a broad and potent TH1-biased immune response in human as well as rhesus macaque macrophages and DC when compared with control virus. Single-cycle SIV-LMP1 also enhanced antigen presentation by lentiviral vector vaccines, suggesting that LMP1-mediated immune activation may enhance lentiviral vector vaccines against HIV-1.

Original languageEnglish
Article number39
JournalRetrovirology
Volume8
DOIs
StatePublished - May 18 2011

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Membrane Proteins
Vaccines
Viruses
HIV-1
Macrophages
Antigen Presentation
Macaca
Macaca mulatta
Human Herpesvirus 4
Interleukin-8
Interleukin-1
Dendritic Cells
HIV Infections
Interleukin-6
Flow Cytometry
Cytokines
Ligands
Infection
Genes
Proteins

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Gupta, S., Termini, J. M., Niu, L., Kanagavelu, S. K., Rahmberg, A. R., Kornbluth, R. S., ... Stone, G. (2011). Latent Membrane Protein 1 as a molecular adjuvant for single-cycle lentiviral vaccines. Retrovirology, 8, [39]. https://doi.org/10.1186/1742-4690-8-39

Latent Membrane Protein 1 as a molecular adjuvant for single-cycle lentiviral vaccines. / Gupta, Sachin; Termini, James M.; Niu, Liguo; Kanagavelu, Saravana K.; Rahmberg, Andrew R.; Kornbluth, Richard S.; Evans, David T.; Stone, Geoffrey.

In: Retrovirology, Vol. 8, 39, 18.05.2011.

Research output: Contribution to journalArticle

Gupta, S, Termini, JM, Niu, L, Kanagavelu, SK, Rahmberg, AR, Kornbluth, RS, Evans, DT & Stone, G 2011, 'Latent Membrane Protein 1 as a molecular adjuvant for single-cycle lentiviral vaccines', Retrovirology, vol. 8, 39. https://doi.org/10.1186/1742-4690-8-39
Gupta S, Termini JM, Niu L, Kanagavelu SK, Rahmberg AR, Kornbluth RS et al. Latent Membrane Protein 1 as a molecular adjuvant for single-cycle lentiviral vaccines. Retrovirology. 2011 May 18;8. 39. https://doi.org/10.1186/1742-4690-8-39
Gupta, Sachin ; Termini, James M. ; Niu, Liguo ; Kanagavelu, Saravana K. ; Rahmberg, Andrew R. ; Kornbluth, Richard S. ; Evans, David T. ; Stone, Geoffrey. / Latent Membrane Protein 1 as a molecular adjuvant for single-cycle lentiviral vaccines. In: Retrovirology. 2011 ; Vol. 8.
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