Laser trabeculoplasty induces changes in the trabecular meshwork glycoproteome: A pilot study

Adriana Amelinckx, Maria Castello, Esdras Arrieta-Quintero, Tinthu Lee, Nelson Salas, Eleut Hernandez, Richard K. Lee, Sanjoy K. Bhattacharya, Jean Marie A. Parel

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Laser trabeculoplasty (LT) is a commonly used modality of treatment for glaucoma. The mechanism by which LT lowers the intraocular pressure (IOP) is unknown. With the use of cat eyes, selective laser trabeculoplasty (SLT) with a Q-switched frequency doubled Nd:YAG laser was used to treat the trabecular meshwork (TM). Laser treated TM was then subjected to proteomic analysis for detection of molecular changes and histological analysis for the detection of structural and protein expression patterns. In addition, the protein glycosylation patterns of laser treated and nontreated TM was assessed and differentially glycosylated proteins were proteomically identified. SLT laser treatment to the TM resulted in elevated glycosylation levels compared to nonlasered TM. TM laser treatment also resulted in protein expression levels changes of several proteins. Elevated levels of biglycan, keratocan and prolargin were detected in laser treated TM compared to nonlasered controls. Further investigation is anticipated to provide insight into how glycosylation changes affect TM proteins and TM regulation of aqueous outflow in response to laser trabeculoplasty.

Original languageEnglish (US)
Pages (from-to)3727-3736
Number of pages10
JournalJournal of Proteome Research
Volume8
Issue number7
DOIs
StatePublished - Jul 6 2009

Keywords

  • Glaucoma
  • Laser trabeculoplasty
  • Proteoglycans
  • Trabecular Meshwork

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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