Laser flow cytometry and cancer chemotherapy

Detection of intracellular anthracyclines by flow cytometry

A. Krishan, R. Ganapathi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The intracellular distribution of important chemotherapeutic antibiotics belonging to the anthracycline group (e.g. adriamycin) can be detected by laser flow cytometry. The indirect method is based on the interference of these compounds with the binding of propidium iodide to the nuclear DNA. While in the direct method, the intracellular fluorescence of these antibiotics is excited and detected with a laser beam in a flow system. The present report demonstrates the use of these two methods for intracellular detection and quantitation of a number of important anthracyclines.

Original languageEnglish
Pages (from-to)1655-1656
Number of pages2
JournalJournal of Histochemistry and Cytochemistry
Volume27
Issue number12
StatePublished - Dec 1 1979
Externally publishedYes

Fingerprint

Anthracyclines
Flow Cytometry
Lasers
Drug Therapy
Anti-Bacterial Agents
Neoplasms
Propidium
Doxorubicin
Fluorescence
DNA

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Laser flow cytometry and cancer chemotherapy : Detection of intracellular anthracyclines by flow cytometry. / Krishan, A.; Ganapathi, R.

In: Journal of Histochemistry and Cytochemistry, Vol. 27, No. 12, 01.12.1979, p. 1655-1656.

Research output: Contribution to journalArticle

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