Abstract
A new experimental therapy for squamous carcinoma was tested by sensitizing human tumor cells with light-sensitive anticancer drugs followed by laser illumination at visible or infrared wavelengths. The anthrapyrazole DUP-941 and the isoquinoline derivative DUP-840 were compared with the dianthraquinone hypericin. P3 human squamous carcinoma cells were incubated for 2 h with the drugs at escalating doses ranging from 5 to 100 μg/ml, then exposed to visible green 532-nm or infrared 1064-nm light at 300 J output from a KTP/Nd:YAG laser. Tumor cell toxicity measured by in vitro MTT viability assays was minimal after DUP-840 uptake but was slightly enhanced by infrared laser emissions. By contrast, the strong tumoricidal effects seen after DUP-941 uptake were amplified over 10-fold by 532-nm light and up to 2- fold by 1064-nm light. Hypericin-sensitized tumor cells were killed after 532 nm irradiation even at the lowest drag dose but were not affected by 1064-nm illumination. The results suggest that laser chemotherapy with drugs sensitive to photothermal energy could become a useful new treatment modality for cancer.
| Original language | English |
|---|---|
| Pages (from-to) | 15-21 |
| Number of pages | 7 |
| Journal | Journal of Clinical Laser Medicine and Surgery |
| Volume | 15 |
| Issue number | 1 |
| State | Published - Feb 1 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biomedical Engineering
- Surgery
Cite this
Laser chemotherapy of human carcinoma cells with three new anticancer drugs. / Eshraghi, Adrien; Castro, D. J.; Paiva, M. B.; Grabber, I. P.; Jongewaard, N.; Arshadnia, S.; Lamas, G.; Soudant, J.; Saxton, R. E.
In: Journal of Clinical Laser Medicine and Surgery, Vol. 15, No. 1, 01.02.1997, p. 15-21.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Laser chemotherapy of human carcinoma cells with three new anticancer drugs
AU - Eshraghi, Adrien
AU - Castro, D. J.
AU - Paiva, M. B.
AU - Grabber, I. P.
AU - Jongewaard, N.
AU - Arshadnia, S.
AU - Lamas, G.
AU - Soudant, J.
AU - Saxton, R. E.
PY - 1997/2/1
Y1 - 1997/2/1
N2 - A new experimental therapy for squamous carcinoma was tested by sensitizing human tumor cells with light-sensitive anticancer drugs followed by laser illumination at visible or infrared wavelengths. The anthrapyrazole DUP-941 and the isoquinoline derivative DUP-840 were compared with the dianthraquinone hypericin. P3 human squamous carcinoma cells were incubated for 2 h with the drugs at escalating doses ranging from 5 to 100 μg/ml, then exposed to visible green 532-nm or infrared 1064-nm light at 300 J output from a KTP/Nd:YAG laser. Tumor cell toxicity measured by in vitro MTT viability assays was minimal after DUP-840 uptake but was slightly enhanced by infrared laser emissions. By contrast, the strong tumoricidal effects seen after DUP-941 uptake were amplified over 10-fold by 532-nm light and up to 2- fold by 1064-nm light. Hypericin-sensitized tumor cells were killed after 532 nm irradiation even at the lowest drag dose but were not affected by 1064-nm illumination. The results suggest that laser chemotherapy with drugs sensitive to photothermal energy could become a useful new treatment modality for cancer.
AB - A new experimental therapy for squamous carcinoma was tested by sensitizing human tumor cells with light-sensitive anticancer drugs followed by laser illumination at visible or infrared wavelengths. The anthrapyrazole DUP-941 and the isoquinoline derivative DUP-840 were compared with the dianthraquinone hypericin. P3 human squamous carcinoma cells were incubated for 2 h with the drugs at escalating doses ranging from 5 to 100 μg/ml, then exposed to visible green 532-nm or infrared 1064-nm light at 300 J output from a KTP/Nd:YAG laser. Tumor cell toxicity measured by in vitro MTT viability assays was minimal after DUP-840 uptake but was slightly enhanced by infrared laser emissions. By contrast, the strong tumoricidal effects seen after DUP-941 uptake were amplified over 10-fold by 532-nm light and up to 2- fold by 1064-nm light. Hypericin-sensitized tumor cells were killed after 532 nm irradiation even at the lowest drag dose but were not affected by 1064-nm illumination. The results suggest that laser chemotherapy with drugs sensitive to photothermal energy could become a useful new treatment modality for cancer.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031080680&partnerID=8YFLogxK
M3 - Article
VL - 15
SP - 15
EP - 21
JO - Photomedicine and Laser Surgery
JF - Photomedicine and Laser Surgery
SN - 1549-5418
IS - 1
ER -