Laser chemotherapy of human carcinoma cells with three new anticancer drugs

A. A. Eshraghi, D. J. Castro, M. B. Paiva, I. P. Grabber, N. Jongewaard, S. Arshadnia, G. Lamas, J. Soudant, R. E. Saxton

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A new experimental therapy for squamous carcinoma was tested by sensitizing human tumor cells with light-sensitive anticancer drugs followed by laser illumination at visible or infrared wavelengths. The anthrapyrazole DUP-941 and the isoquinoline derivative DUP-840 were compared with the dianthraquinone hypericin. P3 human squamous carcinoma cells were incubated for 2 h with the drugs at escalating doses ranging from 5 to 100 μg/ml, then exposed to visible green 532-nm or infrared 1064-nm light at 300 J output from a KTP/Nd:YAG laser. Tumor cell toxicity measured by in vitro MTT viability assays was minimal after DUP-840 uptake but was slightly enhanced by infrared laser emissions. By contrast, the strong tumoricidal effects seen after DUP-941 uptake were amplified over 10-fold by 532-nm light and up to 2- fold by 1064-nm light. Hypericin-sensitized tumor cells were killed after 532 nm irradiation even at the lowest drag dose but were not affected by 1064-nm illumination. The results suggest that laser chemotherapy with drugs sensitive to photothermal energy could become a useful new treatment modality for cancer.

Original languageEnglish (US)
Pages (from-to)15-21
Number of pages7
JournalJournal of Clinical Laser Medicine and Surgery
Volume15
Issue number1
StatePublished - Feb 1 1997
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Biomedical Engineering

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    Eshraghi, A. A., Castro, D. J., Paiva, M. B., Grabber, I. P., Jongewaard, N., Arshadnia, S., Lamas, G., Soudant, J., & Saxton, R. E. (1997). Laser chemotherapy of human carcinoma cells with three new anticancer drugs. Journal of Clinical Laser Medicine and Surgery, 15(1), 15-21.