Large-scale purification of Na,K-ATPase and its protein subunits from lamb kidney medulla.

L. K. Lane, J. D. Potter, J. H. Collins

Research output: Contribution to journalArticle

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Abstract

Procedures are described for the large-scale isolation of purified Na,K-ATPase (EC 3.6.1.3) from frozen lamb kidney outer medulla and for the separation of its two protein subunits by hydroxyapatite chromatography in sodium dodecyl sulfate (SDS). The methods described permit the routine isolation of up to 800 mg of purified Na,K-ATPase in one week, which can subsequently be separated into 500 mg of mr = 95,000 catalytic subunit and 200 mg of glycoprotein with four SDS-hydroxyapatite column runs.

Original languageEnglish
Pages (from-to)157-170
Number of pages14
JournalPreparative Biochemistry
Volume9
Issue number2
StatePublished - Jan 1 1979

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Kidney Medulla
Protein Subunits
Durapatite
Sodium Dodecyl Sulfate
Purification
Adenosine Triphosphatases
Chromatography
Catalytic Domain
Glycoproteins
sodium-translocating ATPase
magnesium sodium potassium ATPase

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Large-scale purification of Na,K-ATPase and its protein subunits from lamb kidney medulla. / Lane, L. K.; Potter, J. D.; Collins, J. H.

In: Preparative Biochemistry, Vol. 9, No. 2, 01.01.1979, p. 157-170.

Research output: Contribution to journalArticle

Lane, L. K. ; Potter, J. D. ; Collins, J. H. / Large-scale purification of Na,K-ATPase and its protein subunits from lamb kidney medulla. In: Preparative Biochemistry. 1979 ; Vol. 9, No. 2. pp. 157-170.
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