Large-scale mapping of human protein interactome using structural complexes

Manoj Tyagi, Kosuke Hashimoto, Benjamin A. Shoemaker, Stefan Wuchty, Anna R. Panchenko

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


Although the identification of protein interactions by high-throughput (HTP) methods progresses at a fast pace, interactome data sets still suffer from high rates of false positives and low coverage. To map the human protein interactome, we describe a new framework that uses experimental evidence on structural complexes, the atomic details of binding interfaces and evolutionary conservation. The structurally inferred interaction network is highly modular and more functionally coherent compared with experimental interaction networks derived from multiple literature citations. Moreover, structurally inferred and high-confidence HTP networks complement each other well, allowing us to construct a merged network to generate testable hypotheses and provide valuable experimental leads.

Original languageEnglish (US)
Pages (from-to)266-271
Number of pages6
JournalEMBO Reports
Issue number3
StatePublished - Mar 2012
Externally publishedYes


  • IBIS
  • protein complexes
  • protein interactions
  • protein structures

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics


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