Large-scale extraction and characterization of CD271+ multipotential stromal cells from trabecular bone in health and osteoarthritis: Implications for bone regeneration strategies based on uncultured or minimally cultured multipotential stromal cells

E. Jones, A. English, S. M. Churchman, D. Kouroupis, S. A. Boxall, S. Kinsey, P. G. Giannoudis, P. Emery, D. McGonagle

Research output: Contribution to journalArticlepeer-review

124 Scopus citations

Abstract

Objective. To test the hypothesis that CD45lowCD271+ bone marrow multipotential stromal cells (MSCs) are abundant in the trabecular bone niche and to explore their functional "fitness" in health and osteoarthritis (OA). Methods. Following enzymatic extraction, MSC release was evaluated using colony-forming unit-fibroblast CFU-F) and colony-forming unit-osteoblast assays, flow cytometry, and confocal microscopy. CD45lowCD271+ cells isolated by fluorescence-activated cell sorting were enumerated and expanded under standard and clonal conditions. Their proliferative and osteogenic potencies were assessed in relation to donor age and compared with those of aspirated CD45lowCD271+ cells. In vitro and in vivo MSC "aging" was measured using quantitative polymerase chain reaction-based telomere length analysis, and standard differentiation assays were utilized to demonstrate multipotentiality.

Original languageEnglish (US)
Pages (from-to)1944-1954
Number of pages11
JournalArthritis and Rheumatism
Volume62
Issue number7
DOIs
StatePublished - Jul 2010
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

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