Large-scale cryosectioning technique allows in vitro correlation of magnetic resonance imaging and gross histologic findings in the wrist

A. L. Makowski, E. A. Ouellette, W. Puckett, Alexander Marcillo

Research output: Contribution to journalArticle

Abstract

To enhance the usefulness of magnetic resonance imaging (MRI) in the clinical assessment of wrist joint pathology, we correlated findings from consecutive MRIs of cadaver wrists with findings from gross histologic sections of these wrists, using the sections as the standard of reference. The study required a sectioning technique that allowed preparation of large- scale sections closely matching corresponding MRIs in size and sectioning plane. We adapted a large-scale cryosectioning technique for undecalcified bone, previously described in the literature, to our study of 16 formalin fixed cadaver wrists. After subjecting each wrist to MR imaging with a wrist coil, we cryosectioned the entire wrist joint, from the distal ulna and radius to the proximal metacarpals, in the frontal plane, which corresponded to the MR images. The cryostat's tissue stage was mounted on a ball and socket-like joint that allowed corrections of the sectioning plane, enabling us to obtain sections in a plane closely resembling the corresponding MRIs. We stained the cryosections by a safranin-O fast green method modified to minimize hydration. When cryosections whose portions detached from the slide during staining were eliminated, morphologic comparison of the remaining coverslipped sections with photos of the corresponding tissue block surface taken before sectioning revealed that the sections were accurate representations of the wrist. The sections were deemed suitable as a standard of reference for comparison of the corresponding MRIs. The large-scale sectioning technique and modified staining method can be used in further comparison studies aimed at refining MRI techniques to permit greater differentiation of joint structures on the MRI.

Original languageEnglish (US)
Pages (from-to)225-229
Number of pages5
JournalJournal of Histotechnology
Volume21
Issue number3
StatePublished - 1998

Fingerprint

Cryoultramicrotomy
Wrist
Magnetic Resonance Imaging
Wrist Joint
Cadaver
Joints
Staining and Labeling
Ulna
Metacarpal Bones
Formaldehyde
In Vitro Techniques
Pathology
Bone and Bones

Keywords

  • Articular cartilage
  • Flexible tissue-block orientation
  • Histology
  • Large- scale sections
  • Ligament
  • MRI
  • Wrist

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Large-scale cryosectioning technique allows in vitro correlation of magnetic resonance imaging and gross histologic findings in the wrist. / Makowski, A. L.; Ouellette, E. A.; Puckett, W.; Marcillo, Alexander.

In: Journal of Histotechnology, Vol. 21, No. 3, 1998, p. 225-229.

Research output: Contribution to journalArticle

Makowski, A. L. ; Ouellette, E. A. ; Puckett, W. ; Marcillo, Alexander. / Large-scale cryosectioning technique allows in vitro correlation of magnetic resonance imaging and gross histologic findings in the wrist. In: Journal of Histotechnology. 1998 ; Vol. 21, No. 3. pp. 225-229.
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