Large-area molecular patterning with polymer pen lithography

Daniel J. Eichelsdoerfer, Xing Liao, Maria D. Cabezas, William Morris, Boya Radha, Keith A. Brown, Louise R. Giam, Adam B. Braunschweig, Chad A. Mirkin

Research output: Contribution to journalArticle

Abstract

The challenge of constructing surfaces with nanostructured chemical functionality is central to many areas of biology and biotechnology. This protocol describes the steps required for performing molecular printing using polymer pen lithography (PPL), a cantilever-free scanning probe-based technique that can generate sub-100-nm molecular features in a massively parallel fashion. To illustrate how such molecular printing can be used for a variety of biologically relevant applications, we detail the fabrication of the lithographic apparatus and the deposition of two materials, an alkanethiol and a polymer onto a gold and silicon surface, respectively, and show how the present approach can be used to generate nanostructures composed of proteins and metals. Finally, we describe how PPL enables researchers to easily create combinatorial arrays of nanostructures, a powerful approach for high-throughput screening. A typical protocol for fabricating PPL arrays and printing with the arrays takes 48-72 h to complete, including two overnight waiting steps.

Original languageEnglish (US)
Pages (from-to)2548-2560
Number of pages13
JournalNature Protocols
Volume8
Issue number12
DOIs
StatePublished - 2013

Fingerprint

Lithography
Printing
Polymers
Nanostructures
Silicon
Biotechnology
Gold
Screening
Metals
Research Personnel
Throughput
Scanning
Fabrication
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Eichelsdoerfer, D. J., Liao, X., Cabezas, M. D., Morris, W., Radha, B., Brown, K. A., ... Mirkin, C. A. (2013). Large-area molecular patterning with polymer pen lithography. Nature Protocols, 8(12), 2548-2560. https://doi.org/10.1038/nprot2013159

Large-area molecular patterning with polymer pen lithography. / Eichelsdoerfer, Daniel J.; Liao, Xing; Cabezas, Maria D.; Morris, William; Radha, Boya; Brown, Keith A.; Giam, Louise R.; Braunschweig, Adam B.; Mirkin, Chad A.

In: Nature Protocols, Vol. 8, No. 12, 2013, p. 2548-2560.

Research output: Contribution to journalArticle

Eichelsdoerfer, DJ, Liao, X, Cabezas, MD, Morris, W, Radha, B, Brown, KA, Giam, LR, Braunschweig, AB & Mirkin, CA 2013, 'Large-area molecular patterning with polymer pen lithography', Nature Protocols, vol. 8, no. 12, pp. 2548-2560. https://doi.org/10.1038/nprot2013159
Eichelsdoerfer DJ, Liao X, Cabezas MD, Morris W, Radha B, Brown KA et al. Large-area molecular patterning with polymer pen lithography. Nature Protocols. 2013;8(12):2548-2560. https://doi.org/10.1038/nprot2013159
Eichelsdoerfer, Daniel J. ; Liao, Xing ; Cabezas, Maria D. ; Morris, William ; Radha, Boya ; Brown, Keith A. ; Giam, Louise R. ; Braunschweig, Adam B. ; Mirkin, Chad A. / Large-area molecular patterning with polymer pen lithography. In: Nature Protocols. 2013 ; Vol. 8, No. 12. pp. 2548-2560.
@article{fee841bfaf78467e9a3ec2698f7b728b,
title = "Large-area molecular patterning with polymer pen lithography",
abstract = "The challenge of constructing surfaces with nanostructured chemical functionality is central to many areas of biology and biotechnology. This protocol describes the steps required for performing molecular printing using polymer pen lithography (PPL), a cantilever-free scanning probe-based technique that can generate sub-100-nm molecular features in a massively parallel fashion. To illustrate how such molecular printing can be used for a variety of biologically relevant applications, we detail the fabrication of the lithographic apparatus and the deposition of two materials, an alkanethiol and a polymer onto a gold and silicon surface, respectively, and show how the present approach can be used to generate nanostructures composed of proteins and metals. Finally, we describe how PPL enables researchers to easily create combinatorial arrays of nanostructures, a powerful approach for high-throughput screening. A typical protocol for fabricating PPL arrays and printing with the arrays takes 48-72 h to complete, including two overnight waiting steps.",
author = "Eichelsdoerfer, {Daniel J.} and Xing Liao and Cabezas, {Maria D.} and William Morris and Boya Radha and Brown, {Keith A.} and Giam, {Louise R.} and Braunschweig, {Adam B.} and Mirkin, {Chad A.}",
year = "2013",
doi = "10.1038/nprot2013159",
language = "English (US)",
volume = "8",
pages = "2548--2560",
journal = "Nature Protocols",
issn = "1754-2189",
publisher = "Nature Publishing Group",
number = "12",

}

TY - JOUR

T1 - Large-area molecular patterning with polymer pen lithography

AU - Eichelsdoerfer, Daniel J.

AU - Liao, Xing

AU - Cabezas, Maria D.

AU - Morris, William

AU - Radha, Boya

AU - Brown, Keith A.

AU - Giam, Louise R.

AU - Braunschweig, Adam B.

AU - Mirkin, Chad A.

PY - 2013

Y1 - 2013

N2 - The challenge of constructing surfaces with nanostructured chemical functionality is central to many areas of biology and biotechnology. This protocol describes the steps required for performing molecular printing using polymer pen lithography (PPL), a cantilever-free scanning probe-based technique that can generate sub-100-nm molecular features in a massively parallel fashion. To illustrate how such molecular printing can be used for a variety of biologically relevant applications, we detail the fabrication of the lithographic apparatus and the deposition of two materials, an alkanethiol and a polymer onto a gold and silicon surface, respectively, and show how the present approach can be used to generate nanostructures composed of proteins and metals. Finally, we describe how PPL enables researchers to easily create combinatorial arrays of nanostructures, a powerful approach for high-throughput screening. A typical protocol for fabricating PPL arrays and printing with the arrays takes 48-72 h to complete, including two overnight waiting steps.

AB - The challenge of constructing surfaces with nanostructured chemical functionality is central to many areas of biology and biotechnology. This protocol describes the steps required for performing molecular printing using polymer pen lithography (PPL), a cantilever-free scanning probe-based technique that can generate sub-100-nm molecular features in a massively parallel fashion. To illustrate how such molecular printing can be used for a variety of biologically relevant applications, we detail the fabrication of the lithographic apparatus and the deposition of two materials, an alkanethiol and a polymer onto a gold and silicon surface, respectively, and show how the present approach can be used to generate nanostructures composed of proteins and metals. Finally, we describe how PPL enables researchers to easily create combinatorial arrays of nanostructures, a powerful approach for high-throughput screening. A typical protocol for fabricating PPL arrays and printing with the arrays takes 48-72 h to complete, including two overnight waiting steps.

UR - http://www.scopus.com/inward/record.url?scp=84888355315&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84888355315&partnerID=8YFLogxK

U2 - 10.1038/nprot2013159

DO - 10.1038/nprot2013159

M3 - Article

C2 - 24263094

VL - 8

SP - 2548

EP - 2560

JO - Nature Protocols

JF - Nature Protocols

SN - 1754-2189

IS - 12

ER -