Langmuir and Langmuir - Blodgett films of organophosphorus acid anhydrolase

Sarita V. Mello, Mustapha Mabrouki, Xihui Cao, Roger M. Leblanc, Tu Chen Cheng, Joseph J. DeFrank

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

In this paper, we describe the preparation and characterization of Langmuir and Langmuir-Blodgett (LB) monolayers of the enzyme organophosphorus acid anhydrolase (OPAA). Langmuir films of OPAA were characterized on different subphases, such as phosphate, ammonium carbonate, and bis-tris-propane buffers. Monolayers at the air-water interface were characterized by measuring the surface pressure and surface potential-area isotherms. In situ UV-vis absorption spectra were also recorded from the Langmuir monolayers. The enzyme activity at the air-water interface was tested by the addition of diisopropylfluorophosphate (DFP) to the subphase. LB films of OPAA were transferred to mica substrates to be studied by atomic force microscopy. Finally, a one-layer LB film of OPAA labeled with a fluorescent probe, fluorescein isothiocyanate (FITC), was deposited onto a quartz slide to be tested as sensor for DFP. The clear, pronounced response and the stability of the LB film as a DFP sensor show the potential of this system as a biosensor.

Original languageEnglish (US)
Pages (from-to)968-973
Number of pages6
JournalBiomacromolecules
Volume4
Issue number4
DOIs
StatePublished - Jul 1 2003

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Langmuir and Langmuir - Blodgett films of organophosphorus acid anhydrolase'. Together they form a unique fingerprint.

  • Cite this

    Mello, S. V., Mabrouki, M., Cao, X., Leblanc, R. M., Cheng, T. C., & DeFrank, J. J. (2003). Langmuir and Langmuir - Blodgett films of organophosphorus acid anhydrolase. Biomacromolecules, 4(4), 968-973. https://doi.org/10.1021/bm025775+