Landing rate measurements to detect fibrinogen adsorption to non-fouling surfaces

Ashutosh Agarwal, Elizabeth Luria, Xiaopei Deng, Joerg Lahann, Henry Hess

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Rapid advances in non-fouling surface technology have pushed the performance of novel coatings toward the detection limit of established protein density quantification techniques. Hence, there is an urgent need for more sensitive detection strategies. Previously we demonstrated that landing rate measurements of microtubules can reveal kinesin surface coverages between 0.1 and 10/im∼ 2. In this report, we quantify the binding kinetics of highly fluorescent markers to surface-adhered proteins and demonstrate the of protein surface densities in the range of 0.1-1000 [m T. We utilize this technique to measure kinesin densities on casein-coated glass surfaces and fibrinogen densities on non-fouling polyethylene glycol methacrylate (PEGMA) surfaces. The use of nanospheres (i) potentially permits the detection of a variety of adsorbed proteins, (ii) facilitates the determination of the landing rate due to their uniformity, and (iii) extends the dynamic range of the method due to their small size.

Original languageEnglish (US)
Pages (from-to)320-326
Number of pages7
JournalCellular and Molecular Bioengineering
Volume5
Issue number3
DOIs
StatePublished - Sep 2012
Externally publishedYes

Keywords

  • Biomaterials
  • Coatings
  • Fibrinogen
  • Protein adsorption

ASJC Scopus subject areas

  • Modeling and Simulation
  • Biochemistry, Genetics and Molecular Biology(all)

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