Laminin-coated electrodes improve cochlear implant function and post-insertion neuronal survival

Esperanza Bas Infante, Mir R. Anwar, Stefania Goncalves, Christine T Dinh, Olena R. Bracho, Juan A. Chiossone, Thomas R. Van De Water

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The benefits of Cochlear implant (CI)technology depend among other factors on the proximity of the electrode array to the spiral ganglion neurons. Laminin, a component of the extracellular matrix, regulates Schwann cell proliferation and survival as well as reorganization of actin fibers within their cytoskeleton, which is necessary for myelination of peripheral axons. In this study we explore the effectiveness of laminin-coated electrodes in promoting neuritic outgrowth from auditory neurons towards the electrode array and the ability to reduce acoustic and electric auditory brainstem response (i.e. aABR and eABR)thresholds. In vitro: Schwann cells and neurites are attracted towards laminin-coated surfaces with longer neuritic processes in laminin-coated dishes compared to uncoated dishes. In vivo: Animals implanted with laminin-coated electrodes experience significant decreases in eABR and aABR thresholds at selected frequencies compared to the results from the uncoated electrodes group. At 1 month post implantation there were a greater number of spiral ganglion neurons and neuritic processes projecting into the scala tympani of animals implanted with laminin-coated electrodes compared to animals with uncoated electrodes. These data suggest that Schwann cells are attracted towards laminin-coated electrodes and promote neuritic outgrowth/ guidance and promote the survival of spiral ganglion neurons following electrode insertion trauma.

Original languageEnglish (US)
Pages (from-to)97-107
Number of pages11
JournalNeuroscience
Volume410
DOIs
StatePublished - Jul 1 2019

Fingerprint

Cochlear Implants
Laminin
Electrodes
Spiral Ganglion
Schwann Cells
Neurons
Scala Tympani
Brain Stem Auditory Evoked Potentials
Neurites
Cytoskeleton
Acoustics
Extracellular Matrix
Axons
Actins
Cell Survival
Cell Proliferation
Technology
Wounds and Injuries

Keywords

  • cochlear implants
  • laminin
  • neuritic outgrowth/guidance
  • Schwann cells
  • spiral ganglion neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Laminin-coated electrodes improve cochlear implant function and post-insertion neuronal survival. / Bas Infante, Esperanza; Anwar, Mir R.; Goncalves, Stefania; Dinh, Christine T; Bracho, Olena R.; Chiossone, Juan A.; Van De Water, Thomas R.

In: Neuroscience, Vol. 410, 01.07.2019, p. 97-107.

Research output: Contribution to journalArticle

Bas Infante, Esperanza ; Anwar, Mir R. ; Goncalves, Stefania ; Dinh, Christine T ; Bracho, Olena R. ; Chiossone, Juan A. ; Van De Water, Thomas R. / Laminin-coated electrodes improve cochlear implant function and post-insertion neuronal survival. In: Neuroscience. 2019 ; Vol. 410. pp. 97-107.
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