Lactate stimulates vasculogenic stem cells via the thioredoxin system and engages an autocrine activation loop involving hypoxia-inducible factor 1

Tatyana N. Milovanova, Veena M. Bhopale, Elena M. Sorokina, Jonni S. Moore, Thomas K. Hunt, Martin Hauer-Jensen, Omaida C Velazquez, Stephen R. Thom

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Abstract

The recruitment and differentiation of circulating stem/progenitor cells (SPCs) in subcutaneous Matrigel in mice was assessed. There were over one million CD34+ SPCs per Matrigel plug 18 h after Matrigel implantation, and including a polymer to elevate the lactate concentration increased the number of SPCs by 3.6-fold. Intricate CD34+ cell-lined channels were linked to the systemic circulation, and lactate accelerated cell differentiation as evaluated based on surface marker expression and cell cycle entry. CD34+ SPCs from lactate-supplemented Matrigel exhibited significantly higher concentrations of thioredoxin 1 (Trxl) and hypoxia-inducible factor 1 (HIF-1) than cells from unsupplemented Matrigel, whereas Trxl and HIF-1 in CD45+ leukocytes were not elevated by lactate. Results obtained using small inhibitory RNA (siRNA) specific to HIF-1 and mice with conditionally HIF-1 null myeloid cells indicated that SPC recruitment and lactate-mediated effects were dependent on HIF-1. Cells from lactate-supplemented Matrigel had higher concentrations of phosphorylated extracellular signal-regulated kinases 1 and 2, Trx1, Trx reductase (TrxR), vascular endothelial growth factor (VEGF), and stromal cell-derived factor 1 (SDF-1) than cells from unsupplemented Matrigel. SPC recruitment and protein changes were inhibited by siRNA specific to lactate dehydrogenase, TrxR, or HIF-1 and by oxamate, apocynin, U0126, N-acetylcysteine, dithioerythritol, and antibodies to VEGF or SDF-1. Oxidative stress from lactate metabolism by SPCs accelerated further SPC recruitment and differentiation through Trx1-mediated elevations in HIF-1 levels and the subsequent synthesis of HIF-1-dependent growth factors.

Original languageEnglish
Pages (from-to)6248-6261
Number of pages14
JournalMolecular and Cellular Biology
Volume28
Issue number20
DOIs
StatePublished - Oct 1 2008

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Hypoxia-Inducible Factor 1
Thioredoxins
Lactic Acid
Stem Cells
Chemokine CXCL12
Vascular Endothelial Growth Factor A
Cell Differentiation
Oxidoreductases
Dithioerythritol
RNA
Null Lymphocytes
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Acetylcysteine
Myeloid Cells

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Milovanova, T. N., Bhopale, V. M., Sorokina, E. M., Moore, J. S., Hunt, T. K., Hauer-Jensen, M., ... Thom, S. R. (2008). Lactate stimulates vasculogenic stem cells via the thioredoxin system and engages an autocrine activation loop involving hypoxia-inducible factor 1. Molecular and Cellular Biology, 28(20), 6248-6261. https://doi.org/10.1128/MCB.00795-08

Lactate stimulates vasculogenic stem cells via the thioredoxin system and engages an autocrine activation loop involving hypoxia-inducible factor 1. / Milovanova, Tatyana N.; Bhopale, Veena M.; Sorokina, Elena M.; Moore, Jonni S.; Hunt, Thomas K.; Hauer-Jensen, Martin; Velazquez, Omaida C; Thom, Stephen R.

In: Molecular and Cellular Biology, Vol. 28, No. 20, 01.10.2008, p. 6248-6261.

Research output: Contribution to journalArticle

Milovanova, Tatyana N. ; Bhopale, Veena M. ; Sorokina, Elena M. ; Moore, Jonni S. ; Hunt, Thomas K. ; Hauer-Jensen, Martin ; Velazquez, Omaida C ; Thom, Stephen R. / Lactate stimulates vasculogenic stem cells via the thioredoxin system and engages an autocrine activation loop involving hypoxia-inducible factor 1. In: Molecular and Cellular Biology. 2008 ; Vol. 28, No. 20. pp. 6248-6261.
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