Lactate dehydrogenase-B cDNA from the teleost Fundulus heteroclitus: evolutionary implications.

Douglas L Crawford, H. R. Constantino, D. A. Powers

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A cDNA that encodes the heart-type lactate dehydrogenase (LDH-B) from the teleost fish Fundulus heteroclitus was cloned and sequenced. The protein encoded by the cDNA was analyzed in relation to 13 LDH proteins from a variety of taxa. One of the deductions from this analysis is that LDH-B proteins have residues in the active site that are unique and that may be important in determining the biochemistry of the heart-type isozyme. Phylogenetic analysis of the LDH sequences indicates that the branch lengths are greater in lower vertebrates, suggesting that the amino acid replacement rates vary depending on the evolutionary constraints within each taxon. Furthermore, the analysis suggests that LDH-C arose prior to the divergence of the LDH-A and LDH-B isozymes and thus that it is probably ancestral to these isozymes.

Original languageEnglish
Pages (from-to)369-383
Number of pages15
JournalMolecular Biology and Evolution
Volume6
Issue number4
StatePublished - Jul 1 1989
Externally publishedYes

Fingerprint

Fundulidae
Fundulus heteroclitus
teleost
lactate dehydrogenase
isozymes
Complementary DNA
Isoenzymes
protein
heart
proteins
biochemistry
active sites
Proteins
vertebrate
amino acid
replacement
divergence
vertebrates
Biochemistry
phylogenetics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Lactate dehydrogenase-B cDNA from the teleost Fundulus heteroclitus : evolutionary implications. / Crawford, Douglas L; Constantino, H. R.; Powers, D. A.

In: Molecular Biology and Evolution, Vol. 6, No. 4, 01.07.1989, p. 369-383.

Research output: Contribution to journalArticle

@article{34d49bb9373c4d91b44ff1e288df15d5,
title = "Lactate dehydrogenase-B cDNA from the teleost Fundulus heteroclitus: evolutionary implications.",
abstract = "A cDNA that encodes the heart-type lactate dehydrogenase (LDH-B) from the teleost fish Fundulus heteroclitus was cloned and sequenced. The protein encoded by the cDNA was analyzed in relation to 13 LDH proteins from a variety of taxa. One of the deductions from this analysis is that LDH-B proteins have residues in the active site that are unique and that may be important in determining the biochemistry of the heart-type isozyme. Phylogenetic analysis of the LDH sequences indicates that the branch lengths are greater in lower vertebrates, suggesting that the amino acid replacement rates vary depending on the evolutionary constraints within each taxon. Furthermore, the analysis suggests that LDH-C arose prior to the divergence of the LDH-A and LDH-B isozymes and thus that it is probably ancestral to these isozymes.",
author = "Crawford, {Douglas L} and Constantino, {H. R.} and Powers, {D. A.}",
year = "1989",
month = "7",
day = "1",
language = "English",
volume = "6",
pages = "369--383",
journal = "Molecular Biology and Evolution",
issn = "0737-4038",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Lactate dehydrogenase-B cDNA from the teleost Fundulus heteroclitus

T2 - evolutionary implications.

AU - Crawford, Douglas L

AU - Constantino, H. R.

AU - Powers, D. A.

PY - 1989/7/1

Y1 - 1989/7/1

N2 - A cDNA that encodes the heart-type lactate dehydrogenase (LDH-B) from the teleost fish Fundulus heteroclitus was cloned and sequenced. The protein encoded by the cDNA was analyzed in relation to 13 LDH proteins from a variety of taxa. One of the deductions from this analysis is that LDH-B proteins have residues in the active site that are unique and that may be important in determining the biochemistry of the heart-type isozyme. Phylogenetic analysis of the LDH sequences indicates that the branch lengths are greater in lower vertebrates, suggesting that the amino acid replacement rates vary depending on the evolutionary constraints within each taxon. Furthermore, the analysis suggests that LDH-C arose prior to the divergence of the LDH-A and LDH-B isozymes and thus that it is probably ancestral to these isozymes.

AB - A cDNA that encodes the heart-type lactate dehydrogenase (LDH-B) from the teleost fish Fundulus heteroclitus was cloned and sequenced. The protein encoded by the cDNA was analyzed in relation to 13 LDH proteins from a variety of taxa. One of the deductions from this analysis is that LDH-B proteins have residues in the active site that are unique and that may be important in determining the biochemistry of the heart-type isozyme. Phylogenetic analysis of the LDH sequences indicates that the branch lengths are greater in lower vertebrates, suggesting that the amino acid replacement rates vary depending on the evolutionary constraints within each taxon. Furthermore, the analysis suggests that LDH-C arose prior to the divergence of the LDH-A and LDH-B isozymes and thus that it is probably ancestral to these isozymes.

UR - http://www.scopus.com/inward/record.url?scp=0024690076&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024690076&partnerID=8YFLogxK

M3 - Article

C2 - 2615640

AN - SCOPUS:0024690076

VL - 6

SP - 369

EP - 383

JO - Molecular Biology and Evolution

JF - Molecular Biology and Evolution

SN - 0737-4038

IS - 4

ER -