Lactate dehydrogenase-B cDNA from the teleost Fundulus heteroclitus: evolutionary implications.

D. L. Crawford, H. R. Constantino, D. A. Powers

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


A cDNA that encodes the heart-type lactate dehydrogenase (LDH-B) from the teleost fish Fundulus heteroclitus was cloned and sequenced. The protein encoded by the cDNA was analyzed in relation to 13 LDH proteins from a variety of taxa. One of the deductions from this analysis is that LDH-B proteins have residues in the active site that are unique and that may be important in determining the biochemistry of the heart-type isozyme. Phylogenetic analysis of the LDH sequences indicates that the branch lengths are greater in lower vertebrates, suggesting that the amino acid replacement rates vary depending on the evolutionary constraints within each taxon. Furthermore, the analysis suggests that LDH-C arose prior to the divergence of the LDH-A and LDH-B isozymes and thus that it is probably ancestral to these isozymes.

Original languageEnglish (US)
Pages (from-to)369-383
Number of pages15
JournalMolecular biology and evolution
Issue number4
StatePublished - Jul 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics


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