Lack of specificity of cyclosporine immunoassays: Results of a College of American Pathologists study

Steven J. Soldin; Bernard W. Steele; David L. Witte; Edward Wang; Ronald J. Elin

Lack of specificity of cyclosporine immunoassays: Results of a College of American Pathologists study

Steven J. Soldin, Bernard W. Steele, David L. Witte, Edward Wang, Ronald J. Elin

Pathology

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Objective. - To evaluate the cross-reactivity of the 6 most abundant cyclosporine A (CsA) metabolites in commonly used assays for CsA. Design. - Whole blood samples containing either only 62 ng/mL CsA (A) or 62 ng/mL CsA and between 49 and 86 ng/mL of 1 of the 6 most abundant CsA metabolites (B) were lyophilized. One sample of A and 1 of B were mailed to each of the laboratories participating in the College of American Pathologists Proficiency Testing Program quarterly during a 3-year period (1999-2001). Method means and coefficients of variation were calculated for each mailing. Results. - The study showed significant cross-reactivity of metabolites in all the immunoassay systems studied. Overall degree of interference decreased from Abbott TDx polyclonal > Abbott TDx monoclonal > DiaSorin > Syva EMIT. High-performance liquid chromatography methods gave results close to those found using mass spectrometric techniques. Conclusions. - Significant metabolite interference was found to occur with the immunoassay systems studied.

Original language	English (US)
Pages (from-to)	19-22
Number of pages	4
Journal	Archives of Pathology and Laboratory Medicine
Volume	127
Issue number	1
State	Published - Jan 1 2003

ASJC Scopus subject areas

Pathology and Forensic Medicine
Medical Laboratory Technology

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abstract = "Objective. - To evaluate the cross-reactivity of the 6 most abundant cyclosporine A (CsA) metabolites in commonly used assays for CsA. Design. - Whole blood samples containing either only 62 ng/mL CsA (A) or 62 ng/mL CsA and between 49 and 86 ng/mL of 1 of the 6 most abundant CsA metabolites (B) were lyophilized. One sample of A and 1 of B were mailed to each of the laboratories participating in the College of American Pathologists Proficiency Testing Program quarterly during a 3-year period (1999-2001). Method means and coefficients of variation were calculated for each mailing. Results. - The study showed significant cross-reactivity of metabolites in all the immunoassay systems studied. Overall degree of interference decreased from Abbott TDx polyclonal > Abbott TDx monoclonal > DiaSorin > Syva EMIT. High-performance liquid chromatography methods gave results close to those found using mass spectrometric techniques. Conclusions. - Significant metabolite interference was found to occur with the immunoassay systems studied.",

author = "Soldin, {Steven J.} and Steele, {Bernard W.} and Witte, {David L.} and Edward Wang and Elin, {Ronald J.}",

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T2 - Results of a College of American Pathologists study

AU - Soldin, Steven J.

AU - Steele, Bernard W.

AU - Witte, David L.

AU - Wang, Edward

AU - Elin, Ronald J.

PY - 2003/1/1

Y1 - 2003/1/1

N2 - Objective. - To evaluate the cross-reactivity of the 6 most abundant cyclosporine A (CsA) metabolites in commonly used assays for CsA. Design. - Whole blood samples containing either only 62 ng/mL CsA (A) or 62 ng/mL CsA and between 49 and 86 ng/mL of 1 of the 6 most abundant CsA metabolites (B) were lyophilized. One sample of A and 1 of B were mailed to each of the laboratories participating in the College of American Pathologists Proficiency Testing Program quarterly during a 3-year period (1999-2001). Method means and coefficients of variation were calculated for each mailing. Results. - The study showed significant cross-reactivity of metabolites in all the immunoassay systems studied. Overall degree of interference decreased from Abbott TDx polyclonal > Abbott TDx monoclonal > DiaSorin > Syva EMIT. High-performance liquid chromatography methods gave results close to those found using mass spectrometric techniques. Conclusions. - Significant metabolite interference was found to occur with the immunoassay systems studied.

AB - Objective. - To evaluate the cross-reactivity of the 6 most abundant cyclosporine A (CsA) metabolites in commonly used assays for CsA. Design. - Whole blood samples containing either only 62 ng/mL CsA (A) or 62 ng/mL CsA and between 49 and 86 ng/mL of 1 of the 6 most abundant CsA metabolites (B) were lyophilized. One sample of A and 1 of B were mailed to each of the laboratories participating in the College of American Pathologists Proficiency Testing Program quarterly during a 3-year period (1999-2001). Method means and coefficients of variation were calculated for each mailing. Results. - The study showed significant cross-reactivity of metabolites in all the immunoassay systems studied. Overall degree of interference decreased from Abbott TDx polyclonal > Abbott TDx monoclonal > DiaSorin > Syva EMIT. High-performance liquid chromatography methods gave results close to those found using mass spectrometric techniques. Conclusions. - Significant metabolite interference was found to occur with the immunoassay systems studied.

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Lack of specificity of cyclosporine immunoassays: Results of a College of American Pathologists study

Abstract

ASJC Scopus subject areas

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