Abstract
Objective. - To evaluate the cross-reactivity of the 6 most abundant cyclosporine A (CsA) metabolites in commonly used assays for CsA. Design. - Whole blood samples containing either only 62 ng/mL CsA (A) or 62 ng/mL CsA and between 49 and 86 ng/mL of 1 of the 6 most abundant CsA metabolites (B) were lyophilized. One sample of A and 1 of B were mailed to each of the laboratories participating in the College of American Pathologists Proficiency Testing Program quarterly during a 3-year period (1999-2001). Method means and coefficients of variation were calculated for each mailing. Results. - The study showed significant cross-reactivity of metabolites in all the immunoassay systems studied. Overall degree of interference decreased from Abbott TDx polyclonal > Abbott TDx monoclonal > DiaSorin > Syva EMIT. High-performance liquid chromatography methods gave results close to those found using mass spectrometric techniques. Conclusions. - Significant metabolite interference was found to occur with the immunoassay systems studied.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 19-22 |
| Number of pages | 4 |
| Journal | Archives of Pathology and Laboratory Medicine |
| Volume | 127 |
| Issue number | 1 |
| State | Published - Jan 1 2003 |
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Medical Laboratory Technology