Lack of luteolytic effect of D-Trp-6-LH-RH in hypophysectomized rhesus monkeys (Macaca mulatta)

R. H. Asch, C. A. Eddy, Andrew V Schally

Research output: Contribution to journalArticle

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Abstract

Three female rhesus monkeys that were completely hypophysectomized at least 9 months before, were induced to ovulate twice with human menopausal gonadotropins (hMG) and human chorionic gonadotropin (hCG). During the first treatment cycle, D-Trp-6-LH-RH was administered i.m., at a dose of 10 μg (dose previously shown to induce luteolysis in intact rhesus monkeys) on Days 3 and 5 postovulatory. Serum progesterone concentrations and length of the luteal phases were not altered by the administration of agonistic analogue of LHRH. During the second treatment cycle, 1 mg of D-Trp-6-LH-RH was infused into the ovarian circulation over a period of 1 h, and blood was collected from the main ovarian vein prior, during, and after the infusion. No decreases of progesterone concentrations were observed in the ovarian vein blood during the period of this study. Peripheral serum progesterone levels and luteal phase lengths were not different from those of untreated animals from our colony. These results show that the administration of D-Trp-6-LH-RH to hypophysectomized rhesus monkeys did not result in luteolysis nor decreases of progesterone secretion by the corpus luteum. These findings cast doubt upon the theory of possible direct effects of LHRH and analogues at the gonadal level in primates as the mechanism by which they induced paradoxical antifertility processes.

Original languageEnglish
Pages (from-to)963-968
Number of pages6
JournalBiology of Reproduction
Volume25
Issue number5
StatePublished - Dec 1 1981
Externally publishedYes

Fingerprint

Luteolytic Agents
Triptorelin Pamoate
Macaca mulatta
Progesterone
Luteolysis
Luteal Phase
Gonadotropin-Releasing Hormone
Veins
Menotropins
Corpus Luteum
Chorionic Gonadotropin
Serum
Primates

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Lack of luteolytic effect of D-Trp-6-LH-RH in hypophysectomized rhesus monkeys (Macaca mulatta). / Asch, R. H.; Eddy, C. A.; Schally, Andrew V.

In: Biology of Reproduction, Vol. 25, No. 5, 01.12.1981, p. 963-968.

Research output: Contribution to journalArticle

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