Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain

R. Isacson, B. Kull, P. Salmi, C. Wahlestedt

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

The intrastriatal infusions of 'naked' small interfering RNA (siRNA) targeted to dopamine D1 receptors (1.0-10.0 nmol over 3 days) did not reduce dopamine D1 receptor messenger RNA levels or receptor protein, assessed by [125I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D 1 receptor ligand binding could be observed. Conclusion: The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference.

Original languageEnglish (US)
Pages (from-to)173-177
Number of pages5
JournalActa Physiologica Scandinavica
Volume179
Issue number2
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

Keywords

  • Dopamine D receptor
  • RNAi
  • siRNA

ASJC Scopus subject areas

  • Physiology

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