Abstract
The intrastriatal infusions of 'naked' small interfering RNA (siRNA) targeted to dopamine D1 receptors (1.0-10.0 nmol over 3 days) did not reduce dopamine D1 receptor messenger RNA levels or receptor protein, assessed by [125I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D 1 receptor ligand binding could be observed. Conclusion: The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference.
Original language | English (US) |
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Pages (from-to) | 173-177 |
Number of pages | 5 |
Journal | Acta Physiologica Scandinavica |
Volume | 179 |
Issue number | 2 |
DOIs | |
State | Published - Oct 1 2003 |
Externally published | Yes |
Keywords
- Dopamine D receptor
- RNAi
- siRNA
ASJC Scopus subject areas
- Physiology