Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain

R. Isacson; B. Kull; P. Salmi; C. Wahlestedt

doi:10.1046/j.1365-201X.2003.01188.x

Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain

R. Isacson, B. Kull, P. Salmi, C. Wahlestedt

Research output: Contribution to journal › Article

31 Scopus citations

Abstract

The intrastriatal infusions of 'naked' small interfering RNA (siRNA) targeted to dopamine D₁ receptors (1.0-10.0 nmol over 3 days) did not reduce dopamine D₁ receptor messenger RNA levels or receptor protein, assessed by [¹²⁵I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D ₁ receptor ligand binding could be observed. Conclusion: The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference.

Original language	English (US)
Pages (from-to)	173-177
Number of pages	5
Journal	Acta Physiologica Scandinavica
Volume	179
Issue number	2
DOIs	https://doi.org/10.1046/j.1365-201X.2003.01188.x
State	Published - Oct 1 2003
Externally published	Yes

Keywords

Dopamine D receptor
RNAi
siRNA

ASJC Scopus subject areas

Physiology

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Isacson, R., Kull, B., Salmi, P., & Wahlestedt, C. (2003). Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain. Acta Physiologica Scandinavica, 179(2), 173-177. https://doi.org/10.1046/j.1365-201X.2003.01188.x

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