Abstract
The intrastriatal infusions of 'naked' small interfering RNA (siRNA) targeted to dopamine D1 receptors (1.0-10.0 nmol over 3 days) did not reduce dopamine D1 receptor messenger RNA levels or receptor protein, assessed by [125I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D 1 receptor ligand binding could be observed. Conclusion: The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference.
| Original language | English |
|---|---|
| Pages (from-to) | 173-177 |
| Number of pages | 5 |
| Journal | Acta Physiologica Scandinavica |
| Volume | 179 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 1 2003 |
| Externally published | Yes |
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Keywords
- Dopamine D receptor
- RNAi
- siRNA
ASJC Scopus subject areas
- Physiology
Cite this
Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain. / Isacson, R.; Kull, B.; Salmi, P.; Wahlestedt, Claes R.
In: Acta Physiologica Scandinavica, Vol. 179, No. 2, 01.10.2003, p. 173-177.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain
AU - Isacson, R.
AU - Kull, B.
AU - Salmi, P.
AU - Wahlestedt, Claes R
PY - 2003/10/1
Y1 - 2003/10/1
N2 - The intrastriatal infusions of 'naked' small interfering RNA (siRNA) targeted to dopamine D1 receptors (1.0-10.0 nmol over 3 days) did not reduce dopamine D1 receptor messenger RNA levels or receptor protein, assessed by [125I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D 1 receptor ligand binding could be observed. Conclusion: The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference.
AB - The intrastriatal infusions of 'naked' small interfering RNA (siRNA) targeted to dopamine D1 receptors (1.0-10.0 nmol over 3 days) did not reduce dopamine D1 receptor messenger RNA levels or receptor protein, assessed by [125I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D 1 receptor ligand binding could be observed. Conclusion: The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference.
KW - Dopamine D receptor
KW - RNAi
KW - siRNA
UR - http://www.scopus.com/inward/record.url?scp=0141888360&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0141888360&partnerID=8YFLogxK
U2 - 10.1046/j.1365-201X.2003.01188.x
DO - 10.1046/j.1365-201X.2003.01188.x
M3 - Article
C2 - 14510781
AN - SCOPUS:0141888360
VL - 179
SP - 173
EP - 177
JO - Skandinavisches Archiv Für Physiologie
JF - Skandinavisches Archiv Für Physiologie
SN - 0370-839X
IS - 2
ER -