Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain

R. Isacson; B. Kull; P. Salmi; C. Wahlestedt

doi:10.1046/j.1365-201X.2003.01188.x

Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain

R. Isacson, B. Kull, P. Salmi, C. Wahlestedt

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The intrastriatal infusions of 'naked' small interfering RNA (siRNA) targeted to dopamine D₁ receptors (1.0-10.0 nmol over 3 days) did not reduce dopamine D₁ receptor messenger RNA levels or receptor protein, assessed by [¹²⁵I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D ₁ receptor ligand binding could be observed. Conclusion: The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference.

Original language	English (US)
Pages (from-to)	173-177
Number of pages	5
Journal	Acta Physiologica Scandinavica
Volume	179
Issue number	2
DOIs	https://doi.org/10.1046/j.1365-201X.2003.01188.x
State	Published - Oct 1 2003
Externally published	Yes

Keywords

Dopamine D receptor
RNAi
siRNA

ASJC Scopus subject areas

Physiology

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10.1046/j.1365-201X.2003.01188.x

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title = "Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain",

abstract = "The intrastriatal infusions of 'naked' small interfering RNA (siRNA) targeted to dopamine D1 receptors (1.0-10.0 nmol over 3 days) did not reduce dopamine D1 receptor messenger RNA levels or receptor protein, assessed by [125I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D 1 receptor ligand binding could be observed. Conclusion: The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference.",

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AU - Wahlestedt, C.

PY - 2003/10/1

Y1 - 2003/10/1

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AB - The intrastriatal infusions of 'naked' small interfering RNA (siRNA) targeted to dopamine D1 receptors (1.0-10.0 nmol over 3 days) did not reduce dopamine D1 receptor messenger RNA levels or receptor protein, assessed by [125I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D 1 receptor ligand binding could be observed. Conclusion: The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference.

KW - Dopamine D receptor

KW - RNAi

KW - siRNA

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Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain

Abstract

Keywords

ASJC Scopus subject areas

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