Lack of detectable genetic recombination on the X chromosome during the parthenogenetic production of female and male aphids

Dinah F. Hales, Alexandra Wilson, Mathew A. Sloane, Jean Christophe Simon, Jean François Legallic, Paul Sunnucks

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We used polymorphic microsatellite markers to look for recombination during parthenogenetic oogenesis between the X chromosomes of aphids of the tribe Macrosiphini. We examined the X chromosome because it comprises ∼ 25% of the genome and previous cytological observations of chromosome pairing and nucleolar organizer (NOR) heteromorphism suggest recombination, although the same is not true for autosomes. A total of 564 parthenogenetic females of Myzus clones with three distinct reproductive modes (cyclical parthenogenesis, obligate parthenogenesis and obligate parthenogenesis with male production) were genotyped at three informative X-linked loci. Also, parthenogenetically produced males from clones encompassing the full range of male-producing reproductive strategies were genotyped. These included 391 Myzus persicae males that were genotyped at three X-linked loci and 538 males from Sitobion clones that were genotyped at five informative X-linked loci. Our results show no departure from clonality in parthenogenetic generations of aphids of the tribe Macrosiphini: no recombinant genotypes were observed in parthenogenetically produced males or females.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalGenetical Research
Volume79
Issue number3
DOIs
StatePublished - Aug 15 2002
Externally publishedYes

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Aphids
X Chromosome
Genetic Recombination
Parthenogenesis
Clone Cells
Chromosome Pairing
Nucleolus Organizer Region
Oogenesis
Microsatellite Repeats
Genotype
Genome

ASJC Scopus subject areas

  • Genetics

Cite this

Lack of detectable genetic recombination on the X chromosome during the parthenogenetic production of female and male aphids. / Hales, Dinah F.; Wilson, Alexandra; Sloane, Mathew A.; Simon, Jean Christophe; Legallic, Jean François; Sunnucks, Paul.

In: Genetical Research, Vol. 79, No. 3, 15.08.2002, p. 203-209.

Research output: Contribution to journalArticle

Hales, Dinah F. ; Wilson, Alexandra ; Sloane, Mathew A. ; Simon, Jean Christophe ; Legallic, Jean François ; Sunnucks, Paul. / Lack of detectable genetic recombination on the X chromosome during the parthenogenetic production of female and male aphids. In: Genetical Research. 2002 ; Vol. 79, No. 3. pp. 203-209.
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