Abstract
Cytochrome c (cyt c) is a heme-containing protein that participates in electron transport in the respiratory chain and as a signaling molecule in the apoptotic cascade. Hereweaddressed the effect of removing mammalian cyt c on the integrity of the respiratory complexes in mammalian cells. Mitochondria from cyt c knockout mouse cells lacked fully assembled complexes I and IV and had reduced levels of complex III. Aredox-deficient mutant of cyt c was unable to rescue the levels of complexes I and IV. We found that cyt c is associated with both complex IV and respiratory supercomplexes, providing a potential mechanism for the requirement for cyt c in the assembly/stability of complex IV.
| Original language | English |
|---|---|
| Pages (from-to) | 4383-4391 |
| Number of pages | 9 |
| Journal | Journal of Biological Chemistry |
| Volume | 284 |
| Issue number | 7 |
| DOIs | |
| State | Published - Feb 13 2009 |
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ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Molecular Biology
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Lack of cytochrome c in mouse fibroblasts disrupts assembly/stability of respiratory complexes I and IV. / Vempati, Uma D.; Han, Xianlin; Moraes, Carlos T.
In: Journal of Biological Chemistry, Vol. 284, No. 7, 13.02.2009, p. 4383-4391.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lack of cytochrome c in mouse fibroblasts disrupts assembly/stability of respiratory complexes I and IV
AU - Vempati, Uma D.
AU - Han, Xianlin
AU - Moraes, Carlos T
PY - 2009/2/13
Y1 - 2009/2/13
N2 - Cytochrome c (cyt c) is a heme-containing protein that participates in electron transport in the respiratory chain and as a signaling molecule in the apoptotic cascade. Hereweaddressed the effect of removing mammalian cyt c on the integrity of the respiratory complexes in mammalian cells. Mitochondria from cyt c knockout mouse cells lacked fully assembled complexes I and IV and had reduced levels of complex III. Aredox-deficient mutant of cyt c was unable to rescue the levels of complexes I and IV. We found that cyt c is associated with both complex IV and respiratory supercomplexes, providing a potential mechanism for the requirement for cyt c in the assembly/stability of complex IV.
AB - Cytochrome c (cyt c) is a heme-containing protein that participates in electron transport in the respiratory chain and as a signaling molecule in the apoptotic cascade. Hereweaddressed the effect of removing mammalian cyt c on the integrity of the respiratory complexes in mammalian cells. Mitochondria from cyt c knockout mouse cells lacked fully assembled complexes I and IV and had reduced levels of complex III. Aredox-deficient mutant of cyt c was unable to rescue the levels of complexes I and IV. We found that cyt c is associated with both complex IV and respiratory supercomplexes, providing a potential mechanism for the requirement for cyt c in the assembly/stability of complex IV.
UR - http://www.scopus.com/inward/record.url?scp=63249108609&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=63249108609&partnerID=8YFLogxK
U2 - 10.1074/jbc.M805972200
DO - 10.1074/jbc.M805972200
M3 - Article
C2 - 19075019
AN - SCOPUS:63249108609
VL - 284
SP - 4383
EP - 4391
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 7
ER -