Abstract
Cytochrome c (cyt c) is a heme-containing protein that participates in electron transport in the respiratory chain and as a signaling molecule in the apoptotic cascade. Hereweaddressed the effect of removing mammalian cyt c on the integrity of the respiratory complexes in mammalian cells. Mitochondria from cyt c knockout mouse cells lacked fully assembled complexes I and IV and had reduced levels of complex III. Aredox-deficient mutant of cyt c was unable to rescue the levels of complexes I and IV. We found that cyt c is associated with both complex IV and respiratory supercomplexes, providing a potential mechanism for the requirement for cyt c in the assembly/stability of complex IV.
Original language | English (US) |
---|---|
Pages (from-to) | 4383-4391 |
Number of pages | 9 |
Journal | Journal of Biological Chemistry |
Volume | 284 |
Issue number | 7 |
DOIs | |
State | Published - Feb 13 2009 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology