Lack of cytochrome c in mouse fibroblasts disrupts assembly/stability of respiratory complexes I and IV

Uma D. Vempati, Xianlin Han, Carlos T Moraes

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Cytochrome c (cyt c) is a heme-containing protein that participates in electron transport in the respiratory chain and as a signaling molecule in the apoptotic cascade. Hereweaddressed the effect of removing mammalian cyt c on the integrity of the respiratory complexes in mammalian cells. Mitochondria from cyt c knockout mouse cells lacked fully assembled complexes I and IV and had reduced levels of complex III. Aredox-deficient mutant of cyt c was unable to rescue the levels of complexes I and IV. We found that cyt c is associated with both complex IV and respiratory supercomplexes, providing a potential mechanism for the requirement for cyt c in the assembly/stability of complex IV.

Original languageEnglish
Pages (from-to)4383-4391
Number of pages9
JournalJournal of Biological Chemistry
Volume284
Issue number7
DOIs
StatePublished - Feb 13 2009

Fingerprint

Electron Transport Complex I
Fibroblasts
Cytochromes c
Electron Transport
Mitochondria
Electron Transport Complex III
Heme
Knockout Mice
Cells
Molecules
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Lack of cytochrome c in mouse fibroblasts disrupts assembly/stability of respiratory complexes I and IV. / Vempati, Uma D.; Han, Xianlin; Moraes, Carlos T.

In: Journal of Biological Chemistry, Vol. 284, No. 7, 13.02.2009, p. 4383-4391.

Research output: Contribution to journalArticle

@article{dfa036bb61c44d2982a550410fbda641,
title = "Lack of cytochrome c in mouse fibroblasts disrupts assembly/stability of respiratory complexes I and IV",
abstract = "Cytochrome c (cyt c) is a heme-containing protein that participates in electron transport in the respiratory chain and as a signaling molecule in the apoptotic cascade. Hereweaddressed the effect of removing mammalian cyt c on the integrity of the respiratory complexes in mammalian cells. Mitochondria from cyt c knockout mouse cells lacked fully assembled complexes I and IV and had reduced levels of complex III. Aredox-deficient mutant of cyt c was unable to rescue the levels of complexes I and IV. We found that cyt c is associated with both complex IV and respiratory supercomplexes, providing a potential mechanism for the requirement for cyt c in the assembly/stability of complex IV.",
author = "Vempati, {Uma D.} and Xianlin Han and Moraes, {Carlos T}",
year = "2009",
month = "2",
day = "13",
doi = "10.1074/jbc.M805972200",
language = "English",
volume = "284",
pages = "4383--4391",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "7",

}

TY - JOUR

T1 - Lack of cytochrome c in mouse fibroblasts disrupts assembly/stability of respiratory complexes I and IV

AU - Vempati, Uma D.

AU - Han, Xianlin

AU - Moraes, Carlos T

PY - 2009/2/13

Y1 - 2009/2/13

N2 - Cytochrome c (cyt c) is a heme-containing protein that participates in electron transport in the respiratory chain and as a signaling molecule in the apoptotic cascade. Hereweaddressed the effect of removing mammalian cyt c on the integrity of the respiratory complexes in mammalian cells. Mitochondria from cyt c knockout mouse cells lacked fully assembled complexes I and IV and had reduced levels of complex III. Aredox-deficient mutant of cyt c was unable to rescue the levels of complexes I and IV. We found that cyt c is associated with both complex IV and respiratory supercomplexes, providing a potential mechanism for the requirement for cyt c in the assembly/stability of complex IV.

AB - Cytochrome c (cyt c) is a heme-containing protein that participates in electron transport in the respiratory chain and as a signaling molecule in the apoptotic cascade. Hereweaddressed the effect of removing mammalian cyt c on the integrity of the respiratory complexes in mammalian cells. Mitochondria from cyt c knockout mouse cells lacked fully assembled complexes I and IV and had reduced levels of complex III. Aredox-deficient mutant of cyt c was unable to rescue the levels of complexes I and IV. We found that cyt c is associated with both complex IV and respiratory supercomplexes, providing a potential mechanism for the requirement for cyt c in the assembly/stability of complex IV.

UR - http://www.scopus.com/inward/record.url?scp=63249108609&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=63249108609&partnerID=8YFLogxK

U2 - 10.1074/jbc.M805972200

DO - 10.1074/jbc.M805972200

M3 - Article

C2 - 19075019

AN - SCOPUS:63249108609

VL - 284

SP - 4383

EP - 4391

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 7

ER -