Lack of cytochrome c in mouse fibroblasts disrupts assembly/stability of respiratory complexes I and IV

Uma D. Vempati, Xianlin Han, Carlos T. Moraes

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Cytochrome c (cyt c) is a heme-containing protein that participates in electron transport in the respiratory chain and as a signaling molecule in the apoptotic cascade. Hereweaddressed the effect of removing mammalian cyt c on the integrity of the respiratory complexes in mammalian cells. Mitochondria from cyt c knockout mouse cells lacked fully assembled complexes I and IV and had reduced levels of complex III. Aredox-deficient mutant of cyt c was unable to rescue the levels of complexes I and IV. We found that cyt c is associated with both complex IV and respiratory supercomplexes, providing a potential mechanism for the requirement for cyt c in the assembly/stability of complex IV.

Original languageEnglish (US)
Pages (from-to)4383-4391
Number of pages9
JournalJournal of Biological Chemistry
Volume284
Issue number7
DOIs
StatePublished - Feb 13 2009

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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