Laboratory diagnosis of infection status in infants perinatally exposed to human immunodeficiency virus type 1

Morris O. Paul, Surya Tetali, Martin L. Lesser, Elaine J. Abrams, Xue Ping Wang, Regina Kowalski, Mahrukh Bamji, Barbara Napolitano, Leslie Gulick, Saroj Bakshi, Savita G Pahwa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Accurate and timely diagnosis of infection status in infants born to women infected with human immunodeficiency virus (HIV) is of paramount importance. The comparative accuracy of five diagnostic decision rules was evaluated in 208 HIV-exposed infants (32 infected, 176 uninfected) based on laboratory testing during the first 6 months of life. Diagnostic rules A and B, which required single blood samples analyzed by culture and polymerase chain reaction (PCR) (rule A) or culture, PCR, and p24 antigen detection (rule B) were more prone to incorrect diagnoses than were rules requiring 2 blood samples analyzed by a single assay (rule C) or combinations of culture and PCR (rules D and E). Rule D, which used PCR as the initial test, established the most useful algorithm: a positive PCR result followed by a positive culture in the second sample confirmed infected status, while two consecutive negative PCR results reconfirmed as negative at 6 months of age established uninfected status.

Original languageEnglish
Pages (from-to)68-76
Number of pages9
JournalJournal of Infectious Diseases
Volume173
Issue number1
StatePublished - Jan 8 1996
Externally publishedYes

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Laboratory Infection
Clinical Laboratory Techniques
HIV-1
Polymerase Chain Reaction
HIV
Antigens
Infection

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Paul, M. O., Tetali, S., Lesser, M. L., Abrams, E. J., Wang, X. P., Kowalski, R., ... Pahwa, S. G. (1996). Laboratory diagnosis of infection status in infants perinatally exposed to human immunodeficiency virus type 1. Journal of Infectious Diseases, 173(1), 68-76.

Laboratory diagnosis of infection status in infants perinatally exposed to human immunodeficiency virus type 1. / Paul, Morris O.; Tetali, Surya; Lesser, Martin L.; Abrams, Elaine J.; Wang, Xue Ping; Kowalski, Regina; Bamji, Mahrukh; Napolitano, Barbara; Gulick, Leslie; Bakshi, Saroj; Pahwa, Savita G.

In: Journal of Infectious Diseases, Vol. 173, No. 1, 08.01.1996, p. 68-76.

Research output: Contribution to journalArticle

Paul, MO, Tetali, S, Lesser, ML, Abrams, EJ, Wang, XP, Kowalski, R, Bamji, M, Napolitano, B, Gulick, L, Bakshi, S & Pahwa, SG 1996, 'Laboratory diagnosis of infection status in infants perinatally exposed to human immunodeficiency virus type 1', Journal of Infectious Diseases, vol. 173, no. 1, pp. 68-76.
Paul MO, Tetali S, Lesser ML, Abrams EJ, Wang XP, Kowalski R et al. Laboratory diagnosis of infection status in infants perinatally exposed to human immunodeficiency virus type 1. Journal of Infectious Diseases. 1996 Jan 8;173(1):68-76.
Paul, Morris O. ; Tetali, Surya ; Lesser, Martin L. ; Abrams, Elaine J. ; Wang, Xue Ping ; Kowalski, Regina ; Bamji, Mahrukh ; Napolitano, Barbara ; Gulick, Leslie ; Bakshi, Saroj ; Pahwa, Savita G. / Laboratory diagnosis of infection status in infants perinatally exposed to human immunodeficiency virus type 1. In: Journal of Infectious Diseases. 1996 ; Vol. 173, No. 1. pp. 68-76.
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