Labelling patterns of carbon-14 in net plankton during a winter-spring bloom

Gary L. Hitchcock

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Weekly surface samples were collected in lower Narragansett Bay, Rhode Island, during the 1975 winter-spring bloom and fractionated by nets to nannoplankton (<20 μm) and total (< 158 μm) size fractions. Each size fraction was assayed for paniculate carbon, nitrogen, carbohydrate, protein, chlorophyll a, and cell counts. The <20 μm values were subtracted from the <158 μm values to estimate the composition of the 20 μm to 158 μm fraction (termed net plankton). As nutrients (primarily nitrogen) decreased to undetectable levels with the culmination of the diatom bloom, the ratios of protein/carbohydrate, carbohydrate/carbon, and carbon/chlorophyll a in the net plankton indicated the diatom population was increasingly nutrient-limited. Each size fraction was also incubated at a saturating light intensity with carbon-14; following filtration, the cells were extracted with solvents to obtain labelled polysaccharide and protein. The daily rates of polysaccharide and protein synthesis in the net plankton declined as the bloom entered the stationary phase. When the diatom population was at its maximum density the majority of the carbon-14 was found in the ethanol-soluble fraction; this may be due to high light intensities or nutrient effects.

Original languageEnglish
Pages (from-to)141-153
Number of pages13
JournalJournal of Experimental Marine Biology and Ecology
Volume31
Issue number2
DOIs
StatePublished - Jan 1 1978

Fingerprint

carbon isotope
plankton
algal bloom
carbohydrate
protein
diatom
winter
carbon
polysaccharide
light intensity
Bacillariophyceae
nutrient
chlorophyll a
carbohydrates
nutrients
polysaccharides
nanoplankton
nitrogen
ethanol
algal blooms

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

Labelling patterns of carbon-14 in net plankton during a winter-spring bloom. / Hitchcock, Gary L.

In: Journal of Experimental Marine Biology and Ecology, Vol. 31, No. 2, 01.01.1978, p. 141-153.

Research output: Contribution to journalArticle

@article{791236d054fe4d939f36aa60324d914a,
title = "Labelling patterns of carbon-14 in net plankton during a winter-spring bloom",
abstract = "Weekly surface samples were collected in lower Narragansett Bay, Rhode Island, during the 1975 winter-spring bloom and fractionated by nets to nannoplankton (<20 μm) and total (< 158 μm) size fractions. Each size fraction was assayed for paniculate carbon, nitrogen, carbohydrate, protein, chlorophyll a, and cell counts. The <20 μm values were subtracted from the <158 μm values to estimate the composition of the 20 μm to 158 μm fraction (termed net plankton). As nutrients (primarily nitrogen) decreased to undetectable levels with the culmination of the diatom bloom, the ratios of protein/carbohydrate, carbohydrate/carbon, and carbon/chlorophyll a in the net plankton indicated the diatom population was increasingly nutrient-limited. Each size fraction was also incubated at a saturating light intensity with carbon-14; following filtration, the cells were extracted with solvents to obtain labelled polysaccharide and protein. The daily rates of polysaccharide and protein synthesis in the net plankton declined as the bloom entered the stationary phase. When the diatom population was at its maximum density the majority of the carbon-14 was found in the ethanol-soluble fraction; this may be due to high light intensities or nutrient effects.",
author = "Hitchcock, {Gary L.}",
year = "1978",
month = "1",
day = "1",
doi = "10.1016/0022-0981(78)90126-0",
language = "English",
volume = "31",
pages = "141--153",
journal = "Journal of Experimental Marine Biology and Ecology",
issn = "0022-0981",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Labelling patterns of carbon-14 in net plankton during a winter-spring bloom

AU - Hitchcock, Gary L.

PY - 1978/1/1

Y1 - 1978/1/1

N2 - Weekly surface samples were collected in lower Narragansett Bay, Rhode Island, during the 1975 winter-spring bloom and fractionated by nets to nannoplankton (<20 μm) and total (< 158 μm) size fractions. Each size fraction was assayed for paniculate carbon, nitrogen, carbohydrate, protein, chlorophyll a, and cell counts. The <20 μm values were subtracted from the <158 μm values to estimate the composition of the 20 μm to 158 μm fraction (termed net plankton). As nutrients (primarily nitrogen) decreased to undetectable levels with the culmination of the diatom bloom, the ratios of protein/carbohydrate, carbohydrate/carbon, and carbon/chlorophyll a in the net plankton indicated the diatom population was increasingly nutrient-limited. Each size fraction was also incubated at a saturating light intensity with carbon-14; following filtration, the cells were extracted with solvents to obtain labelled polysaccharide and protein. The daily rates of polysaccharide and protein synthesis in the net plankton declined as the bloom entered the stationary phase. When the diatom population was at its maximum density the majority of the carbon-14 was found in the ethanol-soluble fraction; this may be due to high light intensities or nutrient effects.

AB - Weekly surface samples were collected in lower Narragansett Bay, Rhode Island, during the 1975 winter-spring bloom and fractionated by nets to nannoplankton (<20 μm) and total (< 158 μm) size fractions. Each size fraction was assayed for paniculate carbon, nitrogen, carbohydrate, protein, chlorophyll a, and cell counts. The <20 μm values were subtracted from the <158 μm values to estimate the composition of the 20 μm to 158 μm fraction (termed net plankton). As nutrients (primarily nitrogen) decreased to undetectable levels with the culmination of the diatom bloom, the ratios of protein/carbohydrate, carbohydrate/carbon, and carbon/chlorophyll a in the net plankton indicated the diatom population was increasingly nutrient-limited. Each size fraction was also incubated at a saturating light intensity with carbon-14; following filtration, the cells were extracted with solvents to obtain labelled polysaccharide and protein. The daily rates of polysaccharide and protein synthesis in the net plankton declined as the bloom entered the stationary phase. When the diatom population was at its maximum density the majority of the carbon-14 was found in the ethanol-soluble fraction; this may be due to high light intensities or nutrient effects.

UR - http://www.scopus.com/inward/record.url?scp=0001426694&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001426694&partnerID=8YFLogxK

U2 - 10.1016/0022-0981(78)90126-0

DO - 10.1016/0022-0981(78)90126-0

M3 - Article

AN - SCOPUS:0001426694

VL - 31

SP - 141

EP - 153

JO - Journal of Experimental Marine Biology and Ecology

JF - Journal of Experimental Marine Biology and Ecology

SN - 0022-0981

IS - 2

ER -