Labeled quantitative mass spectrometry to study the host response during aspergillosis in the common bottlenose dolphin (Tursiops truncatus)

Guillaume Desoubeaux, Maria del Carmen Piqueras, Carolina Le-Bert, Vanessa Fravel, Tonya Clauss, Alexa J. Delaune, Risa Daniels, Eric D. Jensen, Jennifer E. Flower, Gregory D. Bossart, Sanjoy K Bhattacharya, Carolyn Cray

Research output: Contribution to journalArticle

Abstract

Aspergillosis is a fungal infection caused by Aspergillus molds that can affect both humans and animals. Despite advances in diagnostics and therapy, medical management of this disease remains difficult. Expansion of the basic knowledge regarding its pathophysiology in animals is critical to aid in the identification of new biomarkers of infection for diagnosis and therapeutic targets. For such a purpose, proteomics can be used by addressing protein changes during various disease processes. In the present study, a mass spectrometry analysis based on isobaric tagging for relative and absolute quantitation (iTRAQ ® ) was applied for direct identification and relative quantitation of proteins in blood collected from 32 Aspergillus-diseased common bottlenose dolphins (Tursiops truncatus, 32 samples) in comparison with blood from 55 other dolphins (55 samples from 41 clinically-normal controls and from 14 cetaceans with miscellaneous non-Aspergillus inflammation diseases) and ten convalescent dolphins (28 samples). Sixty-six and 40 proteins were found to be ≥2.0-fold over- and underrepresented versus miscellaneous non-Aspergillus inflammatory dolphins, respectively, and most were confirmed vs. clinically-normal controls and convalescents. Many proteins which play a role in the adaptive immune response were identified, including MHC proteins and others involved in catalytic activity like the NADPH-ubiquinone oxido-reductases. Overall, iTRAQ ® appears to be a convenient proteomic tool greatly suited for exploratory ex vivo studies focusing on pathophysiology. This technique should be considered as a preliminary step before validation of new diagnostic markers.

Original languageEnglish (US)
Pages (from-to)42-49
Number of pages8
JournalVeterinary Microbiology
Volume232
DOIs
StatePublished - May 1 2019

Fingerprint

Common Dolphins
Bottle-Nosed Dolphin
Aspergillosis
aspergillosis
Tursiops truncatus
Dolphins
Mass Spectrometry
dolphins
mass spectrometry
Aspergillus
pathophysiology
Proteomics
proteomics
Proteins
proteins
Electron Transport Complex I
therapeutics
Mycoses
ubiquinones
Adaptive Immunity

Keywords

  • Aspergillosis
  • Dolphin
  • Interleukin
  • Orbitrap
  • Prepronociceptin
  • Protamine P1
  • Tursiops truncates

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Labeled quantitative mass spectrometry to study the host response during aspergillosis in the common bottlenose dolphin (Tursiops truncatus). / Desoubeaux, Guillaume; Piqueras, Maria del Carmen; Le-Bert, Carolina; Fravel, Vanessa; Clauss, Tonya; Delaune, Alexa J.; Daniels, Risa; Jensen, Eric D.; Flower, Jennifer E.; Bossart, Gregory D.; Bhattacharya, Sanjoy K; Cray, Carolyn.

In: Veterinary Microbiology, Vol. 232, 01.05.2019, p. 42-49.

Research output: Contribution to journalArticle

Desoubeaux, G, Piqueras, MDC, Le-Bert, C, Fravel, V, Clauss, T, Delaune, AJ, Daniels, R, Jensen, ED, Flower, JE, Bossart, GD, Bhattacharya, SK & Cray, C 2019, 'Labeled quantitative mass spectrometry to study the host response during aspergillosis in the common bottlenose dolphin (Tursiops truncatus)', Veterinary Microbiology, vol. 232, pp. 42-49. https://doi.org/10.1016/j.vetmic.2019.03.030
Desoubeaux, Guillaume ; Piqueras, Maria del Carmen ; Le-Bert, Carolina ; Fravel, Vanessa ; Clauss, Tonya ; Delaune, Alexa J. ; Daniels, Risa ; Jensen, Eric D. ; Flower, Jennifer E. ; Bossart, Gregory D. ; Bhattacharya, Sanjoy K ; Cray, Carolyn. / Labeled quantitative mass spectrometry to study the host response during aspergillosis in the common bottlenose dolphin (Tursiops truncatus). In: Veterinary Microbiology. 2019 ; Vol. 232. pp. 42-49.
@article{5340bea35d02471aa35ad5339839bae4,
title = "Labeled quantitative mass spectrometry to study the host response during aspergillosis in the common bottlenose dolphin (Tursiops truncatus)",
abstract = "Aspergillosis is a fungal infection caused by Aspergillus molds that can affect both humans and animals. Despite advances in diagnostics and therapy, medical management of this disease remains difficult. Expansion of the basic knowledge regarding its pathophysiology in animals is critical to aid in the identification of new biomarkers of infection for diagnosis and therapeutic targets. For such a purpose, proteomics can be used by addressing protein changes during various disease processes. In the present study, a mass spectrometry analysis based on isobaric tagging for relative and absolute quantitation (iTRAQ {\circledR} ) was applied for direct identification and relative quantitation of proteins in blood collected from 32 Aspergillus-diseased common bottlenose dolphins (Tursiops truncatus, 32 samples) in comparison with blood from 55 other dolphins (55 samples from 41 clinically-normal controls and from 14 cetaceans with miscellaneous non-Aspergillus inflammation diseases) and ten convalescent dolphins (28 samples). Sixty-six and 40 proteins were found to be ≥2.0-fold over- and underrepresented versus miscellaneous non-Aspergillus inflammatory dolphins, respectively, and most were confirmed vs. clinically-normal controls and convalescents. Many proteins which play a role in the adaptive immune response were identified, including MHC proteins and others involved in catalytic activity like the NADPH-ubiquinone oxido-reductases. Overall, iTRAQ {\circledR} appears to be a convenient proteomic tool greatly suited for exploratory ex vivo studies focusing on pathophysiology. This technique should be considered as a preliminary step before validation of new diagnostic markers.",
keywords = "Aspergillosis, Dolphin, Interleukin, Orbitrap, Prepronociceptin, Protamine P1, Tursiops truncates",
author = "Guillaume Desoubeaux and Piqueras, {Maria del Carmen} and Carolina Le-Bert and Vanessa Fravel and Tonya Clauss and Delaune, {Alexa J.} and Risa Daniels and Jensen, {Eric D.} and Flower, {Jennifer E.} and Bossart, {Gregory D.} and Bhattacharya, {Sanjoy K} and Carolyn Cray",
year = "2019",
month = "5",
day = "1",
doi = "10.1016/j.vetmic.2019.03.030",
language = "English (US)",
volume = "232",
pages = "42--49",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",

}

TY - JOUR

T1 - Labeled quantitative mass spectrometry to study the host response during aspergillosis in the common bottlenose dolphin (Tursiops truncatus)

AU - Desoubeaux, Guillaume

AU - Piqueras, Maria del Carmen

AU - Le-Bert, Carolina

AU - Fravel, Vanessa

AU - Clauss, Tonya

AU - Delaune, Alexa J.

AU - Daniels, Risa

AU - Jensen, Eric D.

AU - Flower, Jennifer E.

AU - Bossart, Gregory D.

AU - Bhattacharya, Sanjoy K

AU - Cray, Carolyn

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Aspergillosis is a fungal infection caused by Aspergillus molds that can affect both humans and animals. Despite advances in diagnostics and therapy, medical management of this disease remains difficult. Expansion of the basic knowledge regarding its pathophysiology in animals is critical to aid in the identification of new biomarkers of infection for diagnosis and therapeutic targets. For such a purpose, proteomics can be used by addressing protein changes during various disease processes. In the present study, a mass spectrometry analysis based on isobaric tagging for relative and absolute quantitation (iTRAQ ® ) was applied for direct identification and relative quantitation of proteins in blood collected from 32 Aspergillus-diseased common bottlenose dolphins (Tursiops truncatus, 32 samples) in comparison with blood from 55 other dolphins (55 samples from 41 clinically-normal controls and from 14 cetaceans with miscellaneous non-Aspergillus inflammation diseases) and ten convalescent dolphins (28 samples). Sixty-six and 40 proteins were found to be ≥2.0-fold over- and underrepresented versus miscellaneous non-Aspergillus inflammatory dolphins, respectively, and most were confirmed vs. clinically-normal controls and convalescents. Many proteins which play a role in the adaptive immune response were identified, including MHC proteins and others involved in catalytic activity like the NADPH-ubiquinone oxido-reductases. Overall, iTRAQ ® appears to be a convenient proteomic tool greatly suited for exploratory ex vivo studies focusing on pathophysiology. This technique should be considered as a preliminary step before validation of new diagnostic markers.

AB - Aspergillosis is a fungal infection caused by Aspergillus molds that can affect both humans and animals. Despite advances in diagnostics and therapy, medical management of this disease remains difficult. Expansion of the basic knowledge regarding its pathophysiology in animals is critical to aid in the identification of new biomarkers of infection for diagnosis and therapeutic targets. For such a purpose, proteomics can be used by addressing protein changes during various disease processes. In the present study, a mass spectrometry analysis based on isobaric tagging for relative and absolute quantitation (iTRAQ ® ) was applied for direct identification and relative quantitation of proteins in blood collected from 32 Aspergillus-diseased common bottlenose dolphins (Tursiops truncatus, 32 samples) in comparison with blood from 55 other dolphins (55 samples from 41 clinically-normal controls and from 14 cetaceans with miscellaneous non-Aspergillus inflammation diseases) and ten convalescent dolphins (28 samples). Sixty-six and 40 proteins were found to be ≥2.0-fold over- and underrepresented versus miscellaneous non-Aspergillus inflammatory dolphins, respectively, and most were confirmed vs. clinically-normal controls and convalescents. Many proteins which play a role in the adaptive immune response were identified, including MHC proteins and others involved in catalytic activity like the NADPH-ubiquinone oxido-reductases. Overall, iTRAQ ® appears to be a convenient proteomic tool greatly suited for exploratory ex vivo studies focusing on pathophysiology. This technique should be considered as a preliminary step before validation of new diagnostic markers.

KW - Aspergillosis

KW - Dolphin

KW - Interleukin

KW - Orbitrap

KW - Prepronociceptin

KW - Protamine P1

KW - Tursiops truncates

UR - http://www.scopus.com/inward/record.url?scp=85063886281&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063886281&partnerID=8YFLogxK

U2 - 10.1016/j.vetmic.2019.03.030

DO - 10.1016/j.vetmic.2019.03.030

M3 - Article

C2 - 31030843

AN - SCOPUS:85063886281

VL - 232

SP - 42

EP - 49

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

ER -