Label-free protein recognition two-dimensional array using nanomechanical sensors

Min Yue, Jeanne C. Stachowiak, Henry Lin, Ram Datar, Richard Cote, Arun Majumdar

Research output: Contribution to journalArticle

107 Scopus citations

Abstract

We demonstrate two-dimensional multiplexed real-time, label-free antibody-antigen binding assays by optically detecting nanoscale motions of two-dimensional arrays of microcantilever beams. Prostate specific antigen (PSA) was assayed using antibodies covalently bound to one surface of the cantilevers by two different surface chemistries, while the nonreaction surfaces were passivated by polyethylene glycol)-silane. PSA as low as 1 ng/mL was detected while 2 mg/μl of bovine serum albumin induced only negligible deflection on the cantilevers.

Original languageEnglish (US)
Pages (from-to)520-524
Number of pages5
JournalNano Letters
Volume8
Issue number2
DOIs
StatePublished - Feb 1 2008

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Fingerprint Dive into the research topics of 'Label-free protein recognition two-dimensional array using nanomechanical sensors'. Together they form a unique fingerprint.

  • Cite this