Label-free, electrical detection of the SARS virus n-protein with nanowire biosensors utilizing antibody mimics as capture probes

Fumiaki N. Ishikawa, Hsiao Kang Chang, Marco Curreli, Hsiang I. Liao, C. Anders Olson, Po Chiang Chen, Rui Zhang, Richard W. Roberts, Ren Sun, Richard J. Cote, Mark E. Thompson, Chongwu Zhou

Research output: Contribution to journalArticle

101 Scopus citations

Abstract

Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In 2O 3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 μM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors.

Original languageEnglish (US)
Pages (from-to)1219-1224
Number of pages6
JournalACS Nano
Volume3
Issue number5
DOIs
StatePublished - May 26 2009
Externally publishedYes

Keywords

  • Antibody mimetic protein
  • Biosensor
  • Nanowire
  • Nucleocapsid (N) protein

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

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  • Cite this

    Ishikawa, F. N., Chang, H. K., Curreli, M., Liao, H. I., Olson, C. A., Chen, P. C., Zhang, R., Roberts, R. W., Sun, R., Cote, R. J., Thompson, M. E., & Zhou, C. (2009). Label-free, electrical detection of the SARS virus n-protein with nanowire biosensors utilizing antibody mimics as capture probes. ACS Nano, 3(5), 1219-1224. https://doi.org/10.1021/nn900086c