Label-free, electrical detection of the SARS virus n-protein with nanowire biosensors utilizing antibody mimics as capture probes

Fumiaki N. Ishikawa, Hsiao Kang Chang, Marco Curreli, Hsiang I. Liao, C. Anders Olson, Po Chiang Chen, Rui Zhang, Richard W. Roberts, Ren Sun, Richard J Cote, Mark E. Thompson, Chongwu Zhou

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In 2O 3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 μM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors.

Original languageEnglish
Pages (from-to)1219-1224
Number of pages6
JournalACS Nano
Volume3
Issue number5
DOIs
StatePublished - May 26 2009
Externally publishedYes

Fingerprint

viruses
antibodies
Viruses
bioinstrumentation
Biosensors
Antibodies
Nanowires
Labels
nanowires
proteins
Proteins
probes
Fibronectins
Nucleocapsid
biomarkers
Polypeptides
polypeptides
Biomarkers
Bovine Serum Albumin
albumins

Keywords

  • Antibody mimetic protein
  • Biosensor
  • Nanowire
  • Nucleocapsid (N) protein

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Ishikawa, F. N., Chang, H. K., Curreli, M., Liao, H. I., Olson, C. A., Chen, P. C., ... Zhou, C. (2009). Label-free, electrical detection of the SARS virus n-protein with nanowire biosensors utilizing antibody mimics as capture probes. ACS Nano, 3(5), 1219-1224. https://doi.org/10.1021/nn900086c

Label-free, electrical detection of the SARS virus n-protein with nanowire biosensors utilizing antibody mimics as capture probes. / Ishikawa, Fumiaki N.; Chang, Hsiao Kang; Curreli, Marco; Liao, Hsiang I.; Olson, C. Anders; Chen, Po Chiang; Zhang, Rui; Roberts, Richard W.; Sun, Ren; Cote, Richard J; Thompson, Mark E.; Zhou, Chongwu.

In: ACS Nano, Vol. 3, No. 5, 26.05.2009, p. 1219-1224.

Research output: Contribution to journalArticle

Ishikawa, FN, Chang, HK, Curreli, M, Liao, HI, Olson, CA, Chen, PC, Zhang, R, Roberts, RW, Sun, R, Cote, RJ, Thompson, ME & Zhou, C 2009, 'Label-free, electrical detection of the SARS virus n-protein with nanowire biosensors utilizing antibody mimics as capture probes', ACS Nano, vol. 3, no. 5, pp. 1219-1224. https://doi.org/10.1021/nn900086c
Ishikawa, Fumiaki N. ; Chang, Hsiao Kang ; Curreli, Marco ; Liao, Hsiang I. ; Olson, C. Anders ; Chen, Po Chiang ; Zhang, Rui ; Roberts, Richard W. ; Sun, Ren ; Cote, Richard J ; Thompson, Mark E. ; Zhou, Chongwu. / Label-free, electrical detection of the SARS virus n-protein with nanowire biosensors utilizing antibody mimics as capture probes. In: ACS Nano. 2009 ; Vol. 3, No. 5. pp. 1219-1224.
@article{640f08b5dbd34520ac6853ef03f1a84b,
title = "Label-free, electrical detection of the SARS virus n-protein with nanowire biosensors utilizing antibody mimics as capture probes",
abstract = "Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In 2O 3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 μM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors.",
keywords = "Antibody mimetic protein, Biosensor, Nanowire, Nucleocapsid (N) protein",
author = "Ishikawa, {Fumiaki N.} and Chang, {Hsiao Kang} and Marco Curreli and Liao, {Hsiang I.} and Olson, {C. Anders} and Chen, {Po Chiang} and Rui Zhang and Roberts, {Richard W.} and Ren Sun and Cote, {Richard J} and Thompson, {Mark E.} and Chongwu Zhou",
year = "2009",
month = "5",
day = "26",
doi = "10.1021/nn900086c",
language = "English",
volume = "3",
pages = "1219--1224",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "5",

}

TY - JOUR

T1 - Label-free, electrical detection of the SARS virus n-protein with nanowire biosensors utilizing antibody mimics as capture probes

AU - Ishikawa, Fumiaki N.

AU - Chang, Hsiao Kang

AU - Curreli, Marco

AU - Liao, Hsiang I.

AU - Olson, C. Anders

AU - Chen, Po Chiang

AU - Zhang, Rui

AU - Roberts, Richard W.

AU - Sun, Ren

AU - Cote, Richard J

AU - Thompson, Mark E.

AU - Zhou, Chongwu

PY - 2009/5/26

Y1 - 2009/5/26

N2 - Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In 2O 3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 μM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors.

AB - Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In 2O 3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 μM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors.

KW - Antibody mimetic protein

KW - Biosensor

KW - Nanowire

KW - Nucleocapsid (N) protein

UR - http://www.scopus.com/inward/record.url?scp=66949181440&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66949181440&partnerID=8YFLogxK

U2 - 10.1021/nn900086c

DO - 10.1021/nn900086c

M3 - Article

C2 - 19422193

AN - SCOPUS:66949181440

VL - 3

SP - 1219

EP - 1224

JO - ACS Nano

JF - ACS Nano

SN - 1936-0851

IS - 5

ER -