TY - JOUR
T1 - Label and Immobilization Free Detection and Differentiation of Tumor Cells
AU - Gajasinghe, Rajapaksha
AU - Jones, Michelle
AU - Ince, Tan A.
AU - Tigli, Onur
N1 - Funding Information:
Manuscript received January 4, 2018; accepted February 21, 2018. Date of publication March 8, 2018; date of current version April 9, 2018. This work was supported by the National Science Foundation under Grant ECCS-1349245. The work of T. A. Ince was supported in part by the Breast Cancer Research Foundation and in part by the Sylvester Comprehensive Cancer Center. This paper was presented at the 2016 IEEE Sensors Conference, Orlando, FL, USA. The associate editor coordinating the review of this paper and approving it for publication was Prof. Venkat R. Bhethanabotla. (Corresponding author: Rajapaksha Gajasinghe.) R. Gajasinghe is with the Electrical and Computer Engineering Department, University of Miami, Coral Gables, FL 33146 USA, and also with the Dr. John T. Macdonald Foundation Biomedical Nanotechnology Institute, University of Miami, Coral Gables, FL 33146 USA (e-mail: r.gajasinghe@umiami.edu).
PY - 2018/5/1
Y1 - 2018/5/1
N2 - This paper presents an electrical impedance spectroscopy based microfabricated sensor capable of detecting and differentiating tumor cells at single cell accuracy. Cell labeling, device functionalization, or cell capture were not required. Device consisted of a microfluidic channel etched in silicon with semi-parallel gold electrodes fabricated on channel side-walls. Device was tested with three human tumor cell lines and latex beads. All cell lines and latex beads were successfully detected. Differentiation between tumor cells from different cell lines was shown to be possible by analyzing channel impedance at two signal frequencies. Estimates for population opacity values were statistically calculated to be 1.119, 0.257, 0.664, and 0.332 for latex beads, MDA-MB-231 cells, MCF-7 cells, and SK-OV-3 cells, respectively. This device demonstrates a simple, mass-producible, and cost-effective approach for tumor cell detection in clinical and research procedures.
AB - This paper presents an electrical impedance spectroscopy based microfabricated sensor capable of detecting and differentiating tumor cells at single cell accuracy. Cell labeling, device functionalization, or cell capture were not required. Device consisted of a microfluidic channel etched in silicon with semi-parallel gold electrodes fabricated on channel side-walls. Device was tested with three human tumor cell lines and latex beads. All cell lines and latex beads were successfully detected. Differentiation between tumor cells from different cell lines was shown to be possible by analyzing channel impedance at two signal frequencies. Estimates for population opacity values were statistically calculated to be 1.119, 0.257, 0.664, and 0.332 for latex beads, MDA-MB-231 cells, MCF-7 cells, and SK-OV-3 cells, respectively. This device demonstrates a simple, mass-producible, and cost-effective approach for tumor cell detection in clinical and research procedures.
KW - EIS
KW - Lab on a chip
KW - MCF-7
KW - MDA-MB-231
KW - SK-OV-2
KW - circulating tumor cells
KW - label free
KW - single cell analysis
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U2 - 10.1109/JSEN.2018.2813975
DO - 10.1109/JSEN.2018.2813975
M3 - Article
AN - SCOPUS:85043364001
VL - 18
SP - 3486
EP - 3493
JO - IEEE Sensors Journal
JF - IEEE Sensors Journal
SN - 1530-437X
IS - 9
ER -