L3MBTL1, a Histone-Methylation-Dependent Chromatin Lock

Patrick Trojer, Guohong Li, Robert J. Sims, Alejandro Vaquero, Nagesh Kalakonda, Piernicola Boccuni, Donghoon Lee, Hediye Erdjument-Bromage, Paul Tempst, Stephen D Nimer, Yuh Hwa Wang, Danny Reinberg

Research output: Contribution to journalArticle

241 Citations (Scopus)

Abstract

Distinct histone lysine methylation marks are involved in transcriptional repression linked to the formation and maintenance of facultative heterochromatin, although the underlying mechanisms remain unclear. We demonstrate that the malignant-brain-tumor (MBT) protein L3MBTL1 is in a complex with core histones, histone H1b, HP1γ, and Rb. The MBT domain is structurally related to protein domains that directly bind methylated histone residues. Consistent with this, we found that the L3MBTL1 MBT domains compact nucleosomal arrays dependent on mono- and dimethylation of histone H4 lysine 20 and of histone H1b lysine 26. The MBT domains bind at least two nucleosomes simultaneously, linking repression of transcription to recognition of different histone marks by L3MBTL1. Consistently, L3MBTL1 was found to negatively regulate the expression of a subset of genes regulated by E2F, a factor that interacts with Rb.

Original languageEnglish
Pages (from-to)915-928
Number of pages14
JournalCell
Volume129
Issue number5
DOIs
StatePublished - Jun 1 2007
Externally publishedYes

Fingerprint

Methylation
Histones
Chromatin
Brain Neoplasms
Tumors
Brain
Lysine
Histone Code
Heterochromatin
Nucleosomes
Transcription
Maintenance
Proteins
Genes

Keywords

  • DNA
  • SIGNALING

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Trojer, P., Li, G., Sims, R. J., Vaquero, A., Kalakonda, N., Boccuni, P., ... Reinberg, D. (2007). L3MBTL1, a Histone-Methylation-Dependent Chromatin Lock. Cell, 129(5), 915-928. https://doi.org/10.1016/j.cell.2007.03.048

L3MBTL1, a Histone-Methylation-Dependent Chromatin Lock. / Trojer, Patrick; Li, Guohong; Sims, Robert J.; Vaquero, Alejandro; Kalakonda, Nagesh; Boccuni, Piernicola; Lee, Donghoon; Erdjument-Bromage, Hediye; Tempst, Paul; Nimer, Stephen D; Wang, Yuh Hwa; Reinberg, Danny.

In: Cell, Vol. 129, No. 5, 01.06.2007, p. 915-928.

Research output: Contribution to journalArticle

Trojer, P, Li, G, Sims, RJ, Vaquero, A, Kalakonda, N, Boccuni, P, Lee, D, Erdjument-Bromage, H, Tempst, P, Nimer, SD, Wang, YH & Reinberg, D 2007, 'L3MBTL1, a Histone-Methylation-Dependent Chromatin Lock', Cell, vol. 129, no. 5, pp. 915-928. https://doi.org/10.1016/j.cell.2007.03.048
Trojer P, Li G, Sims RJ, Vaquero A, Kalakonda N, Boccuni P et al. L3MBTL1, a Histone-Methylation-Dependent Chromatin Lock. Cell. 2007 Jun 1;129(5):915-928. https://doi.org/10.1016/j.cell.2007.03.048
Trojer, Patrick ; Li, Guohong ; Sims, Robert J. ; Vaquero, Alejandro ; Kalakonda, Nagesh ; Boccuni, Piernicola ; Lee, Donghoon ; Erdjument-Bromage, Hediye ; Tempst, Paul ; Nimer, Stephen D ; Wang, Yuh Hwa ; Reinberg, Danny. / L3MBTL1, a Histone-Methylation-Dependent Chromatin Lock. In: Cell. 2007 ; Vol. 129, No. 5. pp. 915-928.
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