L1 cell adhesion molecule (L1CAM) as a pathogenetic factor in endometriosis

D. Finas, M. Huszar, A. Agic, S. Dogan, H. Kiefel, S. Riedle, D. Gast, Robert Marcovich, F. Noack, P. Altevogt, M. Fogel, D. Hornung

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

BACKGROUND: Endometriosis is a benign and progressive disease with a high prevalence. Women with endometriosis, especially with atypical endometriosis, have a higher probability for developing ovarian cancer compared with women without endometriosis. The L1 cell adhesion molecule (L1CAM) is over expressed in ovarian and endometrial carcinomas and is associated with a bad prognosis. Here, we have analysed L1CAM expression in endometriosis. METHODS AND RESULTS: In our study with the samples from 79 patients with, and 37 patients without, endometriosis, we found that endometriosis cell lines and short-term cultures of endometrium from women with endometriosis expressed L1CAM at the mRNA and protein level. Quantitative RT-PCR analysis showed that L1CAM was expressed at significantly higher level in the epithelial compartment from patients with endometriosis compared with healthy controls (P = 0.0126). By immunohistochemical staining, 15 of 31 ovarian endometriotic lesions (48%) were shown to have L1CAM-positive staining. Of these 15 L1CAM-positive samples, 13 were atypical endometriotic lesions. Soluble L1 present in the conditioned medium of epithelial endometrium cultures from women with endometriosis was able to stimulate neurite outgrowth as measured in a chicken ganglion assay. CONCLUSIONS: We propose that L1CAM could promote endometriosis development by increasing enervation and aggravation. L1CAM expression is higher in atypical endometriosis compared with normal endometriosis.

Original languageEnglish (US)
Pages (from-to)1053-1062
Number of pages10
JournalHuman Reproduction
Volume23
Issue number5
DOIs
StatePublished - May 2008
Externally publishedYes

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Neural Cell Adhesion Molecule L1
Endometriosis
Endometrium
Staining and Labeling
Endometrial Neoplasms
Conditioned Culture Medium

Keywords

  • Atypical endometriosis
  • Endometriosis
  • Immunohistochemistry
  • L1CAM
  • Quantitative RT-PCR

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Finas, D., Huszar, M., Agic, A., Dogan, S., Kiefel, H., Riedle, S., ... Hornung, D. (2008). L1 cell adhesion molecule (L1CAM) as a pathogenetic factor in endometriosis. Human Reproduction, 23(5), 1053-1062. https://doi.org/10.1093/humrep/den044

L1 cell adhesion molecule (L1CAM) as a pathogenetic factor in endometriosis. / Finas, D.; Huszar, M.; Agic, A.; Dogan, S.; Kiefel, H.; Riedle, S.; Gast, D.; Marcovich, Robert; Noack, F.; Altevogt, P.; Fogel, M.; Hornung, D.

In: Human Reproduction, Vol. 23, No. 5, 05.2008, p. 1053-1062.

Research output: Contribution to journalArticle

Finas, D, Huszar, M, Agic, A, Dogan, S, Kiefel, H, Riedle, S, Gast, D, Marcovich, R, Noack, F, Altevogt, P, Fogel, M & Hornung, D 2008, 'L1 cell adhesion molecule (L1CAM) as a pathogenetic factor in endometriosis', Human Reproduction, vol. 23, no. 5, pp. 1053-1062. https://doi.org/10.1093/humrep/den044
Finas D, Huszar M, Agic A, Dogan S, Kiefel H, Riedle S et al. L1 cell adhesion molecule (L1CAM) as a pathogenetic factor in endometriosis. Human Reproduction. 2008 May;23(5):1053-1062. https://doi.org/10.1093/humrep/den044
Finas, D. ; Huszar, M. ; Agic, A. ; Dogan, S. ; Kiefel, H. ; Riedle, S. ; Gast, D. ; Marcovich, Robert ; Noack, F. ; Altevogt, P. ; Fogel, M. ; Hornung, D. / L1 cell adhesion molecule (L1CAM) as a pathogenetic factor in endometriosis. In: Human Reproduction. 2008 ; Vol. 23, No. 5. pp. 1053-1062.
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AU - Riedle, S.

AU - Gast, D.

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AB - BACKGROUND: Endometriosis is a benign and progressive disease with a high prevalence. Women with endometriosis, especially with atypical endometriosis, have a higher probability for developing ovarian cancer compared with women without endometriosis. The L1 cell adhesion molecule (L1CAM) is over expressed in ovarian and endometrial carcinomas and is associated with a bad prognosis. Here, we have analysed L1CAM expression in endometriosis. METHODS AND RESULTS: In our study with the samples from 79 patients with, and 37 patients without, endometriosis, we found that endometriosis cell lines and short-term cultures of endometrium from women with endometriosis expressed L1CAM at the mRNA and protein level. Quantitative RT-PCR analysis showed that L1CAM was expressed at significantly higher level in the epithelial compartment from patients with endometriosis compared with healthy controls (P = 0.0126). By immunohistochemical staining, 15 of 31 ovarian endometriotic lesions (48%) were shown to have L1CAM-positive staining. Of these 15 L1CAM-positive samples, 13 were atypical endometriotic lesions. Soluble L1 present in the conditioned medium of epithelial endometrium cultures from women with endometriosis was able to stimulate neurite outgrowth as measured in a chicken ganglion assay. CONCLUSIONS: We propose that L1CAM could promote endometriosis development by increasing enervation and aggravation. L1CAM expression is higher in atypical endometriosis compared with normal endometriosis.

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