L-Methionine-DL-sulfoximine induces massive efflux of glutamine from cortical astrocytes in primary culture

Jan Albrecht, Michael D. Norenberg

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

L-Methionine-DL-sulfoximine (MSO) is a potent convulsant which metabolically and morphologogically primarily affects astroglia, and the links between the gliotoxic and neurophysiological effects of MSO are not clear. A 5 min treatment with 3 mM MSO increased the rate of efflux of newly loaded radiolabelled glutamine (Gln) from rat cortical astrocytes in primary culture to more than 400% of the basal efflux. MSO did not affect the efflux of the neurotransmitter amino acids GABA or D-aspartate (a non-metabolizable analogue of L-glutamate), under the same experimental conditions. MSO-induced overflow with Gln, which according to a recent account strongly interacts with the N-methyl-D-aspartate (NMDA) receptor complex, may contribute to the convulsive action of the drug.

Original languageEnglish (US)
Pages (from-to)587-590
Number of pages4
JournalEuropean Journal of Pharmacology
Volume182
Issue number3
DOIs
StatePublished - Jul 17 1990

Keywords

  • Astrocytes
  • D-Aspartate efflux
  • GABA efflux
  • Glutamine efflux
  • L-Methionine-D
  • L-sulfoximine

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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