Abstract
L-Methionine-DL-sulfoximine (MSO) is a potent convulsant which metabolically and morphologogically primarily affects astroglia, and the links between the gliotoxic and neurophysiological effects of MSO are not clear. A 5 min treatment with 3 mM MSO increased the rate of efflux of newly loaded radiolabelled glutamine (Gln) from rat cortical astrocytes in primary culture to more than 400% of the basal efflux. MSO did not affect the efflux of the neurotransmitter amino acids GABA or D-aspartate (a non-metabolizable analogue of L-glutamate), under the same experimental conditions. MSO-induced overflow with Gln, which according to a recent account strongly interacts with the N-methyl-D-aspartate (NMDA) receptor complex, may contribute to the convulsive action of the drug.
Original language | English (US) |
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Pages (from-to) | 587-590 |
Number of pages | 4 |
Journal | European Journal of Pharmacology |
Volume | 182 |
Issue number | 3 |
DOIs | |
State | Published - Jul 17 1990 |
Keywords
- Astrocytes
- D-Aspartate efflux
- GABA efflux
- Glutamine efflux
- L-Methionine-D
- L-sulfoximine
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Pharmacology