Kv7.1 ion channels require a lipid to couple voltage sensing to pore opening

Mark A. Zaydman, Jonathan R. Silva, Kelli Delaloye, Yang Li, Hongwu Liang, Hans P Larsson, Jingyi Shi, Jianmin Cui

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Voltage-gated ion channels generate dynamic ionic currents that are vital to the physiological functions of many tissues. These proteins contain separate voltage-sensing domains, which detect changes in transmembrane voltage, and pore domains, which conduct ions. Coupling of voltage sensing and pore opening is critical to the channel function and has been modeled as a protein-protein interaction between the two domains. Here, we show that coupling in Kv7.1 channels requires the lipid phosphatidylinositol 4,5-bisphosphate (PIP2). We found that voltage-sensing domain activation failed to open the pore in the absence of PIP2. This result is due to loss of coupling because PIP2 was also required for pore opening to affect voltage-sensing domain activation. We identified a critical site for PIP2-dependent coupling at the interface between the voltage-sensing domain and the pore domain. This site is actually a conserved lipid-binding site among different K+ channels, suggesting that lipids play an important role in coupling in many ion channels.

Original languageEnglish
Pages (from-to)13180-13185
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number32
DOIs
StatePublished - Aug 6 2013

Fingerprint

Ion Channels
Lipids
Proteins
Phosphatidylinositols
Binding Sites
Ions

Keywords

  • Channelopathy
  • Electromechanical coupling
  • KCNQ
  • Phosphoinositide
  • Voltage channel

ASJC Scopus subject areas

  • General

Cite this

Kv7.1 ion channels require a lipid to couple voltage sensing to pore opening. / Zaydman, Mark A.; Silva, Jonathan R.; Delaloye, Kelli; Li, Yang; Liang, Hongwu; Larsson, Hans P; Shi, Jingyi; Cui, Jianmin.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 32, 06.08.2013, p. 13180-13185.

Research output: Contribution to journalArticle

Zaydman, Mark A. ; Silva, Jonathan R. ; Delaloye, Kelli ; Li, Yang ; Liang, Hongwu ; Larsson, Hans P ; Shi, Jingyi ; Cui, Jianmin. / Kv7.1 ion channels require a lipid to couple voltage sensing to pore opening. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 32. pp. 13180-13185.
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AU - Larsson, Hans P

AU - Shi, Jingyi

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