KATP channel opener protects neonatal rabbit heart better than St. Thomas' solution

Jun Feng, Hongling Li, Eliot Rosenkranz

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objectives. Myocardial protection with ATP-sensitive potassium channel (KATP channel) openers is as effective as St. Thomas' cardioplegia (StTCP) in adult rabbit hearts. This study compares the effectiveness of the KATP channel opener pinacidil to StTCP in protecting neonatal rabbit hearts exposed to global ischemia. Methods. Seventeen neonatal rabbit hearts (7-9 days old) perfused with Krebs-Henseleit buffer (KHB) on a Langendorff apparatus underwent 90 min of normothermic ischemia. Six (ischemia control) received no pretreatment before or during ischemia. Six others (pinacidil) received a 3-min infusion of 50 μM pinacidil in KHB without StTCP at the onset of ischemia. Five others (StTCP) received a 3-min infusion of StTCP at the onset of ischemia. After 60 min of KHB reperfusion, recovery of left ventricular (LV) performance and coronary flow (CF) were measured and compared to preischemia. A paired t test was used for comparison between drug-treated and untreated groups. Results. Pinacidil-treated hearts had significantly better recovery of left ventricular developed pressure (47 ± 3.8 mmHg vs 32 ± 2.5 mmHg, P < 0.05), contractility (+dP/dtmax; 885.4 ± 74 mmHg vs 643.7 ± 65 mmHg, P < 0.05), left ventricular end diastolic pressure (10.5 ± 0.9 mmHg vs 17.4 ± 1.2 mmHg P < 0.05), compliance (-dP/dtmax; 994.2 ± 86 mmHg vs 673.6 ± 69 mmHg, P < 0.05), and CF (5.9 ± 0.4 ml/min vs 4.2 ± 0.2 ml/min, P < 0.05) compared to ischemic control. StTCP only improved the recovery of -dP/dtmax (877.4 ± 73 mmHg/s vs 673.6 ± 69 mmHg/s, P < 0.05) and CF (5.7 ± 0.3 ml/min vs 4.2 ± 0.2 ml/min, P < 0.05) compared to control. Conclusions. Pinacidil pretreatment provided superior recovery of systolic performance compared to St. Thomas' cardioplegia solution in neonatal hearts. Myocardial protection by pretreatment with the KATP channel opener pinacidil may be a new strategy for myocardial protection during pediatric cardiac surgery.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalJournal of Surgical Research
Volume109
Issue number2
DOIs
StatePublished - Feb 1 2003

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KATP Channels
Induced Heart Arrest
Pinacidil
Rabbits
Ischemia
Ventricular Pressure
Thomas' solution
Thoracic Surgery
Compliance
Reperfusion
Pediatrics
Blood Pressure
Pharmaceutical Preparations

Keywords

  • Cardioplegia
  • Ischemia
  • K channel
  • Pinacidil

ASJC Scopus subject areas

  • Surgery

Cite this

KATP channel opener protects neonatal rabbit heart better than St. Thomas' solution. / Feng, Jun; Li, Hongling; Rosenkranz, Eliot.

In: Journal of Surgical Research, Vol. 109, No. 2, 01.02.2003, p. 69-73.

Research output: Contribution to journalArticle

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abstract = "Objectives. Myocardial protection with ATP-sensitive potassium channel (KATP channel) openers is as effective as St. Thomas' cardioplegia (StTCP) in adult rabbit hearts. This study compares the effectiveness of the KATP channel opener pinacidil to StTCP in protecting neonatal rabbit hearts exposed to global ischemia. Methods. Seventeen neonatal rabbit hearts (7-9 days old) perfused with Krebs-Henseleit buffer (KHB) on a Langendorff apparatus underwent 90 min of normothermic ischemia. Six (ischemia control) received no pretreatment before or during ischemia. Six others (pinacidil) received a 3-min infusion of 50 μM pinacidil in KHB without StTCP at the onset of ischemia. Five others (StTCP) received a 3-min infusion of StTCP at the onset of ischemia. After 60 min of KHB reperfusion, recovery of left ventricular (LV) performance and coronary flow (CF) were measured and compared to preischemia. A paired t test was used for comparison between drug-treated and untreated groups. Results. Pinacidil-treated hearts had significantly better recovery of left ventricular developed pressure (47 ± 3.8 mmHg vs 32 ± 2.5 mmHg, P < 0.05), contractility (+dP/dtmax; 885.4 ± 74 mmHg vs 643.7 ± 65 mmHg, P < 0.05), left ventricular end diastolic pressure (10.5 ± 0.9 mmHg vs 17.4 ± 1.2 mmHg P < 0.05), compliance (-dP/dtmax; 994.2 ± 86 mmHg vs 673.6 ± 69 mmHg, P < 0.05), and CF (5.9 ± 0.4 ml/min vs 4.2 ± 0.2 ml/min, P < 0.05) compared to ischemic control. StTCP only improved the recovery of -dP/dtmax (877.4 ± 73 mmHg/s vs 673.6 ± 69 mmHg/s, P < 0.05) and CF (5.7 ± 0.3 ml/min vs 4.2 ± 0.2 ml/min, P < 0.05) compared to control. Conclusions. Pinacidil pretreatment provided superior recovery of systolic performance compared to St. Thomas' cardioplegia solution in neonatal hearts. Myocardial protection by pretreatment with the KATP channel opener pinacidil may be a new strategy for myocardial protection during pediatric cardiac surgery.",
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N2 - Objectives. Myocardial protection with ATP-sensitive potassium channel (KATP channel) openers is as effective as St. Thomas' cardioplegia (StTCP) in adult rabbit hearts. This study compares the effectiveness of the KATP channel opener pinacidil to StTCP in protecting neonatal rabbit hearts exposed to global ischemia. Methods. Seventeen neonatal rabbit hearts (7-9 days old) perfused with Krebs-Henseleit buffer (KHB) on a Langendorff apparatus underwent 90 min of normothermic ischemia. Six (ischemia control) received no pretreatment before or during ischemia. Six others (pinacidil) received a 3-min infusion of 50 μM pinacidil in KHB without StTCP at the onset of ischemia. Five others (StTCP) received a 3-min infusion of StTCP at the onset of ischemia. After 60 min of KHB reperfusion, recovery of left ventricular (LV) performance and coronary flow (CF) were measured and compared to preischemia. A paired t test was used for comparison between drug-treated and untreated groups. Results. Pinacidil-treated hearts had significantly better recovery of left ventricular developed pressure (47 ± 3.8 mmHg vs 32 ± 2.5 mmHg, P < 0.05), contractility (+dP/dtmax; 885.4 ± 74 mmHg vs 643.7 ± 65 mmHg, P < 0.05), left ventricular end diastolic pressure (10.5 ± 0.9 mmHg vs 17.4 ± 1.2 mmHg P < 0.05), compliance (-dP/dtmax; 994.2 ± 86 mmHg vs 673.6 ± 69 mmHg, P < 0.05), and CF (5.9 ± 0.4 ml/min vs 4.2 ± 0.2 ml/min, P < 0.05) compared to ischemic control. StTCP only improved the recovery of -dP/dtmax (877.4 ± 73 mmHg/s vs 673.6 ± 69 mmHg/s, P < 0.05) and CF (5.7 ± 0.3 ml/min vs 4.2 ± 0.2 ml/min, P < 0.05) compared to control. Conclusions. Pinacidil pretreatment provided superior recovery of systolic performance compared to St. Thomas' cardioplegia solution in neonatal hearts. Myocardial protection by pretreatment with the KATP channel opener pinacidil may be a new strategy for myocardial protection during pediatric cardiac surgery.

AB - Objectives. Myocardial protection with ATP-sensitive potassium channel (KATP channel) openers is as effective as St. Thomas' cardioplegia (StTCP) in adult rabbit hearts. This study compares the effectiveness of the KATP channel opener pinacidil to StTCP in protecting neonatal rabbit hearts exposed to global ischemia. Methods. Seventeen neonatal rabbit hearts (7-9 days old) perfused with Krebs-Henseleit buffer (KHB) on a Langendorff apparatus underwent 90 min of normothermic ischemia. Six (ischemia control) received no pretreatment before or during ischemia. Six others (pinacidil) received a 3-min infusion of 50 μM pinacidil in KHB without StTCP at the onset of ischemia. Five others (StTCP) received a 3-min infusion of StTCP at the onset of ischemia. After 60 min of KHB reperfusion, recovery of left ventricular (LV) performance and coronary flow (CF) were measured and compared to preischemia. A paired t test was used for comparison between drug-treated and untreated groups. Results. Pinacidil-treated hearts had significantly better recovery of left ventricular developed pressure (47 ± 3.8 mmHg vs 32 ± 2.5 mmHg, P < 0.05), contractility (+dP/dtmax; 885.4 ± 74 mmHg vs 643.7 ± 65 mmHg, P < 0.05), left ventricular end diastolic pressure (10.5 ± 0.9 mmHg vs 17.4 ± 1.2 mmHg P < 0.05), compliance (-dP/dtmax; 994.2 ± 86 mmHg vs 673.6 ± 69 mmHg, P < 0.05), and CF (5.9 ± 0.4 ml/min vs 4.2 ± 0.2 ml/min, P < 0.05) compared to ischemic control. StTCP only improved the recovery of -dP/dtmax (877.4 ± 73 mmHg/s vs 673.6 ± 69 mmHg/s, P < 0.05) and CF (5.7 ± 0.3 ml/min vs 4.2 ± 0.2 ml/min, P < 0.05) compared to control. Conclusions. Pinacidil pretreatment provided superior recovery of systolic performance compared to St. Thomas' cardioplegia solution in neonatal hearts. Myocardial protection by pretreatment with the KATP channel opener pinacidil may be a new strategy for myocardial protection during pediatric cardiac surgery.

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