Knockout of glial channel ACD-1 exacerbates sensory deficits in a C. elegans mutant by regulating calcium levels of sensory neurons

Ying Wang, Giulia D'Urso, Laura Bianchi

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Degenerin/ epithelial Na + channels (DEG/ENaCs) are voltage-independent Na + or Na +/Ca 2+ channels expressed in many tissues and are needed for a wide range of physiological functions, including sensory perception and transepithelial Na + transport. In the nervous system, DEG/ENaCs are expressed in both neurons and glia. However, the role of glial vs. neuronal DEG/ENaCs remains unclear. We recently reported the characterization of a novel DEG/ENaC in Caenorhabditis elegans that we named ACD-1. ACD-1 is expressed in glial amphid sheath cells. The glial ACD-1, together with the neuronal DEG/ENaC DEG-1, is necessary for acid avoidance and attraction to lysine. We report presently that knockout of acd-1 in glia exacerbates sensory deficits caused by another mutant: the hypomorphic allele of the cGMP-gated channel subunit tax-2. Furthermore, sensory deficits caused by mutations in Gi protein odr-3 and guanylate cyclase daf-11, which regulate the activity of TAX-2/TAX-4 channels, are worsened by knockout of acd-1. We also show that sensory neurons of acd-1 tax-2(p694) double mutants fail to undergo changes in intracellular Ca 2+ when animals are exposed to low concentrations of attractant. Finally, we show that exogenous expression of TRPV1 in sensory neurons and exposure to capsaicin rescue sensory deficits of acd-1 tax-2(p694) mutants, suggesting that sensory deficits of these mutants are bypassed by increasing neuronal excitability. Our data suggest a role of glial DEG/ENaC channel ACD-1 in supporting neuronal activity.

Original languageEnglish (US)
Pages (from-to)148-158
Number of pages11
JournalJournal of neurophysiology
Volume107
Issue number1
DOIs
StatePublished - Jan 2012

Keywords

  • Chemotaxis
  • Degenerin/epithelial sodium channel
  • Imaging

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

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