Kit Is Expressed by Epithelial Cells In Vivo

Eva M.J. Peters, Marcus Maurer, Vladimir A. Botchkarev, Karin De Masey Jensen, Pia Welker, Glynis A. Scott, Ralf Paus

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

In mammalian skin, stem cell factor (SCF) regulates the proliferation and maturation of mast cells and melanocytes, which are thought to be the only cutaneous cells that express the Kit-tyrosine kinase receptor (Kit) and respond to epithelial and mesenchymal-derived SCF. We previously had noted, however, the presence of Kit + cells in murine hair follicles, in an introepithelial tissue compartiment devoid of melanocytes and mast cells. Here we have identified the nature of this Kit + population of cells in hair follicles of C57BL/6 mice. Anagen hair follicles showed strong Kit immunoreactivity not only in the pigmentary unit above the follicular dermal papilla but also in a much more proximally located, homogenous group of nondendritic, nonmelanized cells. By immunohistochemistry (desmoplakin + / Trp-1-) and electron microscopy (presence of tonofilaments, desmosomes, lack of melanosomes), these Kit + cells were shown to be hair matrix keratinocytes and were also found in melanocyte-deficient hair follicles (KitSl/KitSl-d mice, Kit-neutralizing antibody-treated C57BL/6 mice). Expression of Kit and SCF was strongly hair-cycle-dependent, suggesting a functional role of epithelial Kit expression in hair growth control. This was supported by the observation that mice unable to respond to SCF stimulation (KitW/KitW-v) showed a significant retardation of anagen development compared to their wild-type littermates. The expression of Kit in the most rapidly proliferating compartment of the hair follicle epithelium suggests intriguing, as yet unexplored new functions of Kit signaling in epithelial cell biology.

Original languageEnglish (US)
Pages (from-to)976-984
Number of pages9
JournalJournal of Investigative Dermatology
Volume121
Issue number5
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

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Stem Cell Factor
Hair Follicle
Epithelial Cells
Melanocytes
Hair
Inbred C57BL Mouse
Mast Cells
Desmoplakins
Skin
Cytology
Receptor Protein-Tyrosine Kinases
Melanosomes
Neutralizing Antibodies
Desmosomes
Intermediate Filaments
Electron microscopy
Mesenchymal Stromal Cells
Keratinocytes
Cells
Cell Biology

Keywords

  • 6 mouse
  • C57BL
  • Epithelial cell biology
  • Hair cycling
  • Kit-neutralizing antibody
  • SCF

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Peters, E. M. J., Maurer, M., Botchkarev, V. A., Jensen, K. D. M., Welker, P., Scott, G. A., & Paus, R. (2003). Kit Is Expressed by Epithelial Cells In Vivo. Journal of Investigative Dermatology, 121(5), 976-984. https://doi.org/10.1046/j.1523-1747.2003.12478.x

Kit Is Expressed by Epithelial Cells In Vivo. / Peters, Eva M.J.; Maurer, Marcus; Botchkarev, Vladimir A.; Jensen, Karin De Masey; Welker, Pia; Scott, Glynis A.; Paus, Ralf.

In: Journal of Investigative Dermatology, Vol. 121, No. 5, 01.01.2003, p. 976-984.

Research output: Contribution to journalArticle

Peters, EMJ, Maurer, M, Botchkarev, VA, Jensen, KDM, Welker, P, Scott, GA & Paus, R 2003, 'Kit Is Expressed by Epithelial Cells In Vivo', Journal of Investigative Dermatology, vol. 121, no. 5, pp. 976-984. https://doi.org/10.1046/j.1523-1747.2003.12478.x
Peters EMJ, Maurer M, Botchkarev VA, Jensen KDM, Welker P, Scott GA et al. Kit Is Expressed by Epithelial Cells In Vivo. Journal of Investigative Dermatology. 2003 Jan 1;121(5):976-984. https://doi.org/10.1046/j.1523-1747.2003.12478.x
Peters, Eva M.J. ; Maurer, Marcus ; Botchkarev, Vladimir A. ; Jensen, Karin De Masey ; Welker, Pia ; Scott, Glynis A. ; Paus, Ralf. / Kit Is Expressed by Epithelial Cells In Vivo. In: Journal of Investigative Dermatology. 2003 ; Vol. 121, No. 5. pp. 976-984.
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