Kinetics and quantitation of in-111 labeled platelet deposition on control and heparin-bonded polyurethane angio catheters in a dog model

M. K. Dewanjee, S. M. Rowland, R. P. Robinson, P. K. Dewanjee, J. L. Ha lgovich, M. Kapadvanjwala, D. C. MacGregor, A. N. Serafini, G. M. P alatianos, G. N. Sfakianakis

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1 Scopus citations

Abstract

The dynamics of platelet deposition on control polyurethane catheters (CPC) and heparin-bonded polyurethane catheters (HBPC) were evaluated with In-111 labeled platelets (In-PLT) using a computerized gamma camera (CGC). Ten nonheparinized dogs (18-25 kg) had both femoral arteries catherized with 10 cm of CPC and HBPC (5 Fr.) 24 hr postinjection of 300-420 microcuries of In-PLT, and imaged for 3 hr with a gamma camera. Regional platelet deposition on three segments of catheters and the puncture site was determined. Catheters were harvested and radioactivity on the catheter segments (proximal: PROX, middle: MID, distal: DIST and puncture site: PS) of both was determined. From the platelet count in blood, and radioactivity in blood and segments of catheters, adjacent artery, and area of artery and catheter, the platelet-density [X103 (mean ± S.D.)] on catheter and artery was calculated and tabulated. Proximal values were cath (CPC), 1289 ± 1125; artery, 1355 ± 587; cath (HBPC), 125 ± 113; artery, 1149 ± 1620. The middle values were cath (CPC), 1102 ± 1109; artery, 1512 ± 625; catg (HBPC), 132 ± 108; artery, 1011 ± 942. Cistal values were cath (CPC), 780 ± 584; artery, 132 ± 108; cath (HBPC), 227 ± 194; artery, 1457 ± 1309. The puncture site values were cath (CPC), 106 ± 382; artery, 1011 ± 942; cath (HBPC), 164 ± 135; artery, 1498 ± 1240. The large standard deviation in retained platelets is due to embolization. The platelet-density and regional counts on catheter segments were lower with HBPC than CPC, as was the rate of platelet-deposition. Most of the thrombi were lost during pull-out of the catheter. Both in vivo (dynamic) and in vitro studies were necessary for evaluation of CPC thrombogenicity.

Original languageEnglish (US)
Pages (from-to)365-368
Number of pages4
JournalASAIO Transactions
Volume35
Issue number3
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Biophysics

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    Dewanjee, M. K., Rowland, S. M., Robinson, R. P., Dewanjee, P. K., Ha lgovich, J. L., Kapadvanjwala, M., MacGregor, D. C., Serafini, A. N., P alatianos, G. M., & Sfakianakis, G. N. (1989). Kinetics and quantitation of in-111 labeled platelet deposition on control and heparin-bonded polyurethane angio catheters in a dog model. ASAIO Transactions, 35(3), 365-368. https://doi.org/10.1097/00002216-198907000-00062