Kinetics and quantitation of in-111 labeled platelet deposition on control and heparin-bonded polyurethane angio catheters in a dog model

M. K. Dewanjee, S. M. Rowland, R. P. Robinson, P. K. Dewanjee, J. L. Ha lgovich, M. Kapadvanjwala, D. C. MacGregor, Aldo N Serafini, G. M. P alatianos, George N Sfakianakis

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Abstract

The dynamics of platelet deposition on control polyurethane catheters (CPC) and heparin-bonded polyurethane catheters (HBPC) were evaluated with In-111 labeled platelets (In-PLT) using a computerized gamma camera (CGC). Ten nonheparinized dogs (18-25 kg) had both femoral arteries catherized with 10 cm of CPC and HBPC (5 Fr.) 24 hr postinjection of 300-420 microcuries of In-PLT, and imaged for 3 hr with a gamma camera. Regional platelet deposition on three segments of catheters and the puncture site was determined. Catheters were harvested and radioactivity on the catheter segments (proximal: PROX, middle: MID, distal: DIST and puncture site: PS) of both was determined. From the platelet count in blood, and radioactivity in blood and segments of catheters, adjacent artery, and area of artery and catheter, the platelet-density [X103 (mean ± S.D.)] on catheter and artery was calculated and tabulated. Proximal values were cath (CPC), 1289 ± 1125; artery, 1355 ± 587; cath (HBPC), 125 ± 113; artery, 1149 ± 1620. The middle values were cath (CPC), 1102 ± 1109; artery, 1512 ± 625; catg (HBPC), 132 ± 108; artery, 1011 ± 942. Cistal values were cath (CPC), 780 ± 584; artery, 132 ± 108; cath (HBPC), 227 ± 194; artery, 1457 ± 1309. The puncture site values were cath (CPC), 106 ± 382; artery, 1011 ± 942; cath (HBPC), 164 ± 135; artery, 1498 ± 1240. The large standard deviation in retained platelets is due to embolization. The platelet-density and regional counts on catheter segments were lower with HBPC than CPC, as was the rate of platelet-deposition. Most of the thrombi were lost during pull-out of the catheter. Both in vivo (dynamic) and in vitro studies were necessary for evaluation of CPC thrombogenicity.

Original languageEnglish
Pages (from-to)365-368
Number of pages4
JournalASAIO Transactions
Volume35
Issue number3
StatePublished - Jul 1 1989

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Polyurethanes
Catheters
Platelets
Heparin
Blood Platelets
Dogs
Kinetics
Arteries
Punctures
Gamma Cameras
Radioactivity
Blood
Cameras

ASJC Scopus subject areas

  • Biophysics

Cite this

Dewanjee, M. K., Rowland, S. M., Robinson, R. P., Dewanjee, P. K., Ha lgovich, J. L., Kapadvanjwala, M., ... Sfakianakis, G. N. (1989). Kinetics and quantitation of in-111 labeled platelet deposition on control and heparin-bonded polyurethane angio catheters in a dog model. ASAIO Transactions, 35(3), 365-368.

Kinetics and quantitation of in-111 labeled platelet deposition on control and heparin-bonded polyurethane angio catheters in a dog model. / Dewanjee, M. K.; Rowland, S. M.; Robinson, R. P.; Dewanjee, P. K.; Ha lgovich, J. L.; Kapadvanjwala, M.; MacGregor, D. C.; Serafini, Aldo N; P alatianos, G. M.; Sfakianakis, George N.

In: ASAIO Transactions, Vol. 35, No. 3, 01.07.1989, p. 365-368.

Research output: Contribution to journalArticle

Dewanjee, MK, Rowland, SM, Robinson, RP, Dewanjee, PK, Ha lgovich, JL, Kapadvanjwala, M, MacGregor, DC, Serafini, AN, P alatianos, GM & Sfakianakis, GN 1989, 'Kinetics and quantitation of in-111 labeled platelet deposition on control and heparin-bonded polyurethane angio catheters in a dog model', ASAIO Transactions, vol. 35, no. 3, pp. 365-368.
Dewanjee MK, Rowland SM, Robinson RP, Dewanjee PK, Ha lgovich JL, Kapadvanjwala M et al. Kinetics and quantitation of in-111 labeled platelet deposition on control and heparin-bonded polyurethane angio catheters in a dog model. ASAIO Transactions. 1989 Jul 1;35(3):365-368.
Dewanjee, M. K. ; Rowland, S. M. ; Robinson, R. P. ; Dewanjee, P. K. ; Ha lgovich, J. L. ; Kapadvanjwala, M. ; MacGregor, D. C. ; Serafini, Aldo N ; P alatianos, G. M. ; Sfakianakis, George N. / Kinetics and quantitation of in-111 labeled platelet deposition on control and heparin-bonded polyurethane angio catheters in a dog model. In: ASAIO Transactions. 1989 ; Vol. 35, No. 3. pp. 365-368.
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abstract = "The dynamics of platelet deposition on control polyurethane catheters (CPC) and heparin-bonded polyurethane catheters (HBPC) were evaluated with In-111 labeled platelets (In-PLT) using a computerized gamma camera (CGC). Ten nonheparinized dogs (18-25 kg) had both femoral arteries catherized with 10 cm of CPC and HBPC (5 Fr.) 24 hr postinjection of 300-420 microcuries of In-PLT, and imaged for 3 hr with a gamma camera. Regional platelet deposition on three segments of catheters and the puncture site was determined. Catheters were harvested and radioactivity on the catheter segments (proximal: PROX, middle: MID, distal: DIST and puncture site: PS) of both was determined. From the platelet count in blood, and radioactivity in blood and segments of catheters, adjacent artery, and area of artery and catheter, the platelet-density [X103 (mean ± S.D.)] on catheter and artery was calculated and tabulated. Proximal values were cath (CPC), 1289 ± 1125; artery, 1355 ± 587; cath (HBPC), 125 ± 113; artery, 1149 ± 1620. The middle values were cath (CPC), 1102 ± 1109; artery, 1512 ± 625; catg (HBPC), 132 ± 108; artery, 1011 ± 942. Cistal values were cath (CPC), 780 ± 584; artery, 132 ± 108; cath (HBPC), 227 ± 194; artery, 1457 ± 1309. The puncture site values were cath (CPC), 106 ± 382; artery, 1011 ± 942; cath (HBPC), 164 ± 135; artery, 1498 ± 1240. The large standard deviation in retained platelets is due to embolization. The platelet-density and regional counts on catheter segments were lower with HBPC than CPC, as was the rate of platelet-deposition. Most of the thrombi were lost during pull-out of the catheter. Both in vivo (dynamic) and in vitro studies were necessary for evaluation of CPC thrombogenicity.",
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AU - Ha lgovich, J. L.

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