Kinetic states and modes of single large-conductance calcium-activated potassium channels in cultured rat skeletal muscle

O. B. McManus, Karl Magleby

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

Kinetic states and modes of a large-conductance Ca2+-activated K+ channel in excised patches of membrane from cultured rat skeletal muscle were studied with the patch clamp technique. Up to 106 open and shut intervals were analysed from each of seven different excised membrane patches containing a single channel. Plots of the mean durations of consecutive groups of ten to fifty open and shut intervals were made to assess kinetic stability of the channel. Occasional abrupt decreases in the mean open interval duration from normal to different distinct levels, which were maintained for hundreds to thousands of consecutive intervals, indicated entry of the channel into different modes. Four different kinetic modes were identified: normal mode, which included 96% of the intervals; intermediate open mode with 3.2% of the intervals; brief open mode with 0.5% of the intervals; and buzz mode with 0.1% of the intervals. The mean open interval durations were 61% of normal during the intermediate open mode, 12% of normal during the brief open mode, and 2.6% of normal during the buzz mode. Most mode transitions were observed from the normal mode to one of the other modes and then back to normal. Sojourns in the normal mode lasted 5-1000 s. Sojourns in the intermediate open, brief open, and buzz modes lasted 1.5-150, 1-7, and 0.01-1 s, respectively. During normal activity the distributions of interval durations were typically described by the sum of three to four exponential components for the open intervals and six to eight exponential components for the shut intervals, and this was the case for data obtained over a wide range of open channel probability resulting from different Ca2+. These observations suggest that the channel can enter at least three to four open and six to eight shut states during normal activity. The numbers of detected states for data sets of different sample sizes drawn from normal activity agreed with theoretical predictions, and were essentially independent of the segment of normal activity from which the data sets were drawn. These observations are consistent with relative stability of channel kinetics during normal activity. Detection of each additional open or shut state after the first was found to require a 3- to 10-fold increase in the number of analysed events. The intermediate open mode differed from the normal mode in that the longest open component of the four normal open components was absent. The brief open mode differed from the normal mode in that the two longest of the four normal open components, the fastest shut component, and possibly one or more intermediate shut components, were absent. The intermediate and brief open modes appeared consistent with conformational changes being restricted to subsets of the same states entered during normal activity. One open state may be excluded for the intermediate open mode, and two open and one or more shut states may be excluded for the brief open mode. The buzz mode differed from the normal mode in that the two longest of the four normal open components were absent, and only three shut components were detected. Ninety-five per cent of the openings in the buzz mode were from the fastest open component, compared to 20% for the brief open mode, 5% for the intermediate mode, and 4% for the normal mode. Fitting the distributions of open and shut intervals with sums of exponentials is consistent with a Markov process for channel kinetics in which there are discrete states, with the rate constants for transitions among the states remaining constant with time. A model which assumed a continuum of states and fractal kinetics was ranked below the Markov model and typically gave very poor descriptions of the distributions of intervals. These results suggest that the large-conductance Ca2+-activated K+ channel can enter three to four discrete open states and six to eight discrete shut states during normal activity. When entry into one or more of the normal states is excluded, activity in the intermediate open or brief open modes occurs. A buzz mode can also be entered.

Original languageEnglish
Pages (from-to)79-120
Number of pages42
JournalJournal of Physiology
Volume402
StatePublished - Jan 1 1988

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Large-Conductance Calcium-Activated Potassium Channels
Skeletal Muscle
Calcium-Activated Potassium Channels
Markov Chains
Fractals
Membranes
Normal Distribution
Patch-Clamp Techniques
Sample Size

ASJC Scopus subject areas

  • Physiology

Cite this

Kinetic states and modes of single large-conductance calcium-activated potassium channels in cultured rat skeletal muscle. / McManus, O. B.; Magleby, Karl.

In: Journal of Physiology, Vol. 402, 01.01.1988, p. 79-120.

Research output: Contribution to journalArticle

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abstract = "Kinetic states and modes of a large-conductance Ca2+-activated K+ channel in excised patches of membrane from cultured rat skeletal muscle were studied with the patch clamp technique. Up to 106 open and shut intervals were analysed from each of seven different excised membrane patches containing a single channel. Plots of the mean durations of consecutive groups of ten to fifty open and shut intervals were made to assess kinetic stability of the channel. Occasional abrupt decreases in the mean open interval duration from normal to different distinct levels, which were maintained for hundreds to thousands of consecutive intervals, indicated entry of the channel into different modes. Four different kinetic modes were identified: normal mode, which included 96{\%} of the intervals; intermediate open mode with 3.2{\%} of the intervals; brief open mode with 0.5{\%} of the intervals; and buzz mode with 0.1{\%} of the intervals. The mean open interval durations were 61{\%} of normal during the intermediate open mode, 12{\%} of normal during the brief open mode, and 2.6{\%} of normal during the buzz mode. Most mode transitions were observed from the normal mode to one of the other modes and then back to normal. Sojourns in the normal mode lasted 5-1000 s. Sojourns in the intermediate open, brief open, and buzz modes lasted 1.5-150, 1-7, and 0.01-1 s, respectively. During normal activity the distributions of interval durations were typically described by the sum of three to four exponential components for the open intervals and six to eight exponential components for the shut intervals, and this was the case for data obtained over a wide range of open channel probability resulting from different Ca2+. These observations suggest that the channel can enter at least three to four open and six to eight shut states during normal activity. The numbers of detected states for data sets of different sample sizes drawn from normal activity agreed with theoretical predictions, and were essentially independent of the segment of normal activity from which the data sets were drawn. These observations are consistent with relative stability of channel kinetics during normal activity. Detection of each additional open or shut state after the first was found to require a 3- to 10-fold increase in the number of analysed events. The intermediate open mode differed from the normal mode in that the longest open component of the four normal open components was absent. The brief open mode differed from the normal mode in that the two longest of the four normal open components, the fastest shut component, and possibly one or more intermediate shut components, were absent. The intermediate and brief open modes appeared consistent with conformational changes being restricted to subsets of the same states entered during normal activity. One open state may be excluded for the intermediate open mode, and two open and one or more shut states may be excluded for the brief open mode. The buzz mode differed from the normal mode in that the two longest of the four normal open components were absent, and only three shut components were detected. Ninety-five per cent of the openings in the buzz mode were from the fastest open component, compared to 20{\%} for the brief open mode, 5{\%} for the intermediate mode, and 4{\%} for the normal mode. Fitting the distributions of open and shut intervals with sums of exponentials is consistent with a Markov process for channel kinetics in which there are discrete states, with the rate constants for transitions among the states remaining constant with time. A model which assumed a continuum of states and fractal kinetics was ranked below the Markov model and typically gave very poor descriptions of the distributions of intervals. These results suggest that the large-conductance Ca2+-activated K+ channel can enter three to four discrete open states and six to eight discrete shut states during normal activity. When entry into one or more of the normal states is excluded, activity in the intermediate open or brief open modes occurs. A buzz mode can also be entered.",
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N2 - Kinetic states and modes of a large-conductance Ca2+-activated K+ channel in excised patches of membrane from cultured rat skeletal muscle were studied with the patch clamp technique. Up to 106 open and shut intervals were analysed from each of seven different excised membrane patches containing a single channel. Plots of the mean durations of consecutive groups of ten to fifty open and shut intervals were made to assess kinetic stability of the channel. Occasional abrupt decreases in the mean open interval duration from normal to different distinct levels, which were maintained for hundreds to thousands of consecutive intervals, indicated entry of the channel into different modes. Four different kinetic modes were identified: normal mode, which included 96% of the intervals; intermediate open mode with 3.2% of the intervals; brief open mode with 0.5% of the intervals; and buzz mode with 0.1% of the intervals. The mean open interval durations were 61% of normal during the intermediate open mode, 12% of normal during the brief open mode, and 2.6% of normal during the buzz mode. Most mode transitions were observed from the normal mode to one of the other modes and then back to normal. Sojourns in the normal mode lasted 5-1000 s. Sojourns in the intermediate open, brief open, and buzz modes lasted 1.5-150, 1-7, and 0.01-1 s, respectively. During normal activity the distributions of interval durations were typically described by the sum of three to four exponential components for the open intervals and six to eight exponential components for the shut intervals, and this was the case for data obtained over a wide range of open channel probability resulting from different Ca2+. These observations suggest that the channel can enter at least three to four open and six to eight shut states during normal activity. The numbers of detected states for data sets of different sample sizes drawn from normal activity agreed with theoretical predictions, and were essentially independent of the segment of normal activity from which the data sets were drawn. These observations are consistent with relative stability of channel kinetics during normal activity. Detection of each additional open or shut state after the first was found to require a 3- to 10-fold increase in the number of analysed events. The intermediate open mode differed from the normal mode in that the longest open component of the four normal open components was absent. The brief open mode differed from the normal mode in that the two longest of the four normal open components, the fastest shut component, and possibly one or more intermediate shut components, were absent. The intermediate and brief open modes appeared consistent with conformational changes being restricted to subsets of the same states entered during normal activity. One open state may be excluded for the intermediate open mode, and two open and one or more shut states may be excluded for the brief open mode. The buzz mode differed from the normal mode in that the two longest of the four normal open components were absent, and only three shut components were detected. Ninety-five per cent of the openings in the buzz mode were from the fastest open component, compared to 20% for the brief open mode, 5% for the intermediate mode, and 4% for the normal mode. Fitting the distributions of open and shut intervals with sums of exponentials is consistent with a Markov process for channel kinetics in which there are discrete states, with the rate constants for transitions among the states remaining constant with time. A model which assumed a continuum of states and fractal kinetics was ranked below the Markov model and typically gave very poor descriptions of the distributions of intervals. These results suggest that the large-conductance Ca2+-activated K+ channel can enter three to four discrete open states and six to eight discrete shut states during normal activity. When entry into one or more of the normal states is excluded, activity in the intermediate open or brief open modes occurs. A buzz mode can also be entered.

AB - Kinetic states and modes of a large-conductance Ca2+-activated K+ channel in excised patches of membrane from cultured rat skeletal muscle were studied with the patch clamp technique. Up to 106 open and shut intervals were analysed from each of seven different excised membrane patches containing a single channel. Plots of the mean durations of consecutive groups of ten to fifty open and shut intervals were made to assess kinetic stability of the channel. Occasional abrupt decreases in the mean open interval duration from normal to different distinct levels, which were maintained for hundreds to thousands of consecutive intervals, indicated entry of the channel into different modes. Four different kinetic modes were identified: normal mode, which included 96% of the intervals; intermediate open mode with 3.2% of the intervals; brief open mode with 0.5% of the intervals; and buzz mode with 0.1% of the intervals. The mean open interval durations were 61% of normal during the intermediate open mode, 12% of normal during the brief open mode, and 2.6% of normal during the buzz mode. Most mode transitions were observed from the normal mode to one of the other modes and then back to normal. Sojourns in the normal mode lasted 5-1000 s. Sojourns in the intermediate open, brief open, and buzz modes lasted 1.5-150, 1-7, and 0.01-1 s, respectively. During normal activity the distributions of interval durations were typically described by the sum of three to four exponential components for the open intervals and six to eight exponential components for the shut intervals, and this was the case for data obtained over a wide range of open channel probability resulting from different Ca2+. These observations suggest that the channel can enter at least three to four open and six to eight shut states during normal activity. The numbers of detected states for data sets of different sample sizes drawn from normal activity agreed with theoretical predictions, and were essentially independent of the segment of normal activity from which the data sets were drawn. These observations are consistent with relative stability of channel kinetics during normal activity. Detection of each additional open or shut state after the first was found to require a 3- to 10-fold increase in the number of analysed events. The intermediate open mode differed from the normal mode in that the longest open component of the four normal open components was absent. The brief open mode differed from the normal mode in that the two longest of the four normal open components, the fastest shut component, and possibly one or more intermediate shut components, were absent. The intermediate and brief open modes appeared consistent with conformational changes being restricted to subsets of the same states entered during normal activity. One open state may be excluded for the intermediate open mode, and two open and one or more shut states may be excluded for the brief open mode. The buzz mode differed from the normal mode in that the two longest of the four normal open components were absent, and only three shut components were detected. Ninety-five per cent of the openings in the buzz mode were from the fastest open component, compared to 20% for the brief open mode, 5% for the intermediate mode, and 4% for the normal mode. Fitting the distributions of open and shut intervals with sums of exponentials is consistent with a Markov process for channel kinetics in which there are discrete states, with the rate constants for transitions among the states remaining constant with time. A model which assumed a continuum of states and fractal kinetics was ranked below the Markov model and typically gave very poor descriptions of the distributions of intervals. These results suggest that the large-conductance Ca2+-activated K+ channel can enter three to four discrete open states and six to eight discrete shut states during normal activity. When entry into one or more of the normal states is excluded, activity in the intermediate open or brief open modes occurs. A buzz mode can also be entered.

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