Kinetic analysis of zinc accumulation in the gills of juvenile rainbow trout: Effects of zinc acclimation and implications for biotic ligand modeling

Derek H. Alsop, Chris M. Wood

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47 Scopus citations

Abstract

Juvenile rainbow trout were acclimated to hard water (Ca2- = 1.0 mM, Mg2+ = 0.2mM; hardness = 120 mg CaCO3/L) and hard water plus 250 μg/L Zn (3.8 μM). After 30 d of exposure, there was no difference in the total Zn levels of the gills of Zn-exposed and control fish (~70 μg Zn/g gill). Exposure of both groups to a range of Zn concentrations (0-2,900 μg/L Zn) for up to 7 d also had no effect on the measured total Zn levels in the gills. However, using radiolabeled 65Zn, measurement of new Zn appearance in the gills was possible. Trout were exposed to a range of Zn concentrations (with 65Zn) and the gills were sampled at times ranging from 0.5 to 72 h. The fast turnover pool of Zn in the gills increased with increasing acute Zn exposure concentration, while the maximum size of the fast pool was about ninefold larger in Zn-acclimated fish (4.14 μg Zn/g gill) versus control fish (0.45 μg Zn/g gill). At all sampling times, gill 65Zn accumulation exhibited saturation kinetics, allowing calculation of binding capacity (Br(max)) and affinity (K(d)). In both control and Zn-acclimated trout, K(d) decreased rapidly (affinity increased) from 0.5 to 3 h and then remained constant up to 72 h. B(max) increased rapidly from 0.5 to 3 h in both groups, then the rate of increase began to subside but was still increasing from 24 to 72 h. At all times, the K(d) of Zn-acclimated fish was higher (i.e., lower affinity) and B(max) was greater than controls. The stabilized K(d)s (>3 h) were approximately 280 μg/L total Zn (log K = 5.6 as Zn2+) and 575 μg/L total Zn (log K = 5.3 as Zn2+) in control and Zn-acclimated fish, respectively. The B(max) of control fish at 0.5 h was 0.37 μg Zn/g gill and increased to 8.63 μg Zn/g gill by 72 h. The B(max) of Zn-acclimated fish increased from 0.70 to 11.61 μg Zn/g gill over the same time period. Preexposure to 250 μg/L Zn appeared to have little effect on acute zinc toxicity, though the 96-h LC50s for both groups were relatively high (~3,000 μg/L Zn) in comparison to previous measurements. The relationship between gill binding constants for different metals and relative toxicity is critically assessed with respect to biotic ligand modeling.

Original languageEnglish (US)
Pages (from-to)1911-1918
Number of pages8
JournalEnvironmental Toxicology and Chemistry
Volume19
Issue number7
DOIs
StatePublished - Jan 1 2000

Keywords

  • Acclimation
  • Biotic ligand modeling
  • Gills
  • Rainbow trout
  • Zinc

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

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