Kinetic analysis of splenic erythropoiesis in mice under prolonged hypoxic stress

Arnold Markoe, J. P. Okunewick, L. M. Schiffer

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The cytokinetics of C3H mouse spleen cells during compensatory erythropoiesis were evaluated by a double labeling autoradiographic method. The total contribution of the spleen to erythropoiesis was also determined utilizing normal and splenectomized mice and evaluating the ensuing peripheral blood changes during hypoxia. Only the basophilic and the definitely erythroid spleen cell populations expanded markedly in response to hypoxia, reaching plateau levels during the first wk of exposure. During this time, the apparent DNA synthesis time [T(S)] and cell cycle time [T(C)] of the basophilic population remained unchanged, but a reduction of these parameters occurred in the erythroid population. T(s) fell from 7.2 to 5.5 h or less and T(C) decreased proportionately, since the labeling index remained unchanged. The authors concluded that the shortening of T(s) and T(C) in some spleen cell populations may be one of the mechanisms subserving the ultimate peripheral blood reticulocytosis and increased hematocrit. However, the spleen of C3H mice apparently functions only in an accessory capacity since the splenectomized animal appears to be capable of full compensation to the erythropoietic stress of prolonged hypoxia.

Original languageEnglish
Pages (from-to)340-349
Number of pages10
JournalExperimental Hematology
Volume1
Issue number6
StatePublished - Dec 1 1973
Externally publishedYes

Fingerprint

Erythropoiesis
Spleen
Inbred C3H Mouse
Population
Reticulocytosis
Erythroid Cells
Hematocrit
Cell Cycle
DNA
Hypoxia

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Kinetic analysis of splenic erythropoiesis in mice under prolonged hypoxic stress. / Markoe, Arnold; Okunewick, J. P.; Schiffer, L. M.

In: Experimental Hematology, Vol. 1, No. 6, 01.12.1973, p. 340-349.

Research output: Contribution to journalArticle

Markoe, Arnold ; Okunewick, J. P. ; Schiffer, L. M. / Kinetic analysis of splenic erythropoiesis in mice under prolonged hypoxic stress. In: Experimental Hematology. 1973 ; Vol. 1, No. 6. pp. 340-349.
@article{2ad2e78c2a8c448f8c9db7ae162e0566,
title = "Kinetic analysis of splenic erythropoiesis in mice under prolonged hypoxic stress",
abstract = "The cytokinetics of C3H mouse spleen cells during compensatory erythropoiesis were evaluated by a double labeling autoradiographic method. The total contribution of the spleen to erythropoiesis was also determined utilizing normal and splenectomized mice and evaluating the ensuing peripheral blood changes during hypoxia. Only the basophilic and the definitely erythroid spleen cell populations expanded markedly in response to hypoxia, reaching plateau levels during the first wk of exposure. During this time, the apparent DNA synthesis time [T(S)] and cell cycle time [T(C)] of the basophilic population remained unchanged, but a reduction of these parameters occurred in the erythroid population. T(s) fell from 7.2 to 5.5 h or less and T(C) decreased proportionately, since the labeling index remained unchanged. The authors concluded that the shortening of T(s) and T(C) in some spleen cell populations may be one of the mechanisms subserving the ultimate peripheral blood reticulocytosis and increased hematocrit. However, the spleen of C3H mice apparently functions only in an accessory capacity since the splenectomized animal appears to be capable of full compensation to the erythropoietic stress of prolonged hypoxia.",
author = "Arnold Markoe and Okunewick, {J. P.} and Schiffer, {L. M.}",
year = "1973",
month = "12",
day = "1",
language = "English",
volume = "1",
pages = "340--349",
journal = "Experimental Hematology",
issn = "0301-472X",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Kinetic analysis of splenic erythropoiesis in mice under prolonged hypoxic stress

AU - Markoe, Arnold

AU - Okunewick, J. P.

AU - Schiffer, L. M.

PY - 1973/12/1

Y1 - 1973/12/1

N2 - The cytokinetics of C3H mouse spleen cells during compensatory erythropoiesis were evaluated by a double labeling autoradiographic method. The total contribution of the spleen to erythropoiesis was also determined utilizing normal and splenectomized mice and evaluating the ensuing peripheral blood changes during hypoxia. Only the basophilic and the definitely erythroid spleen cell populations expanded markedly in response to hypoxia, reaching plateau levels during the first wk of exposure. During this time, the apparent DNA synthesis time [T(S)] and cell cycle time [T(C)] of the basophilic population remained unchanged, but a reduction of these parameters occurred in the erythroid population. T(s) fell from 7.2 to 5.5 h or less and T(C) decreased proportionately, since the labeling index remained unchanged. The authors concluded that the shortening of T(s) and T(C) in some spleen cell populations may be one of the mechanisms subserving the ultimate peripheral blood reticulocytosis and increased hematocrit. However, the spleen of C3H mice apparently functions only in an accessory capacity since the splenectomized animal appears to be capable of full compensation to the erythropoietic stress of prolonged hypoxia.

AB - The cytokinetics of C3H mouse spleen cells during compensatory erythropoiesis were evaluated by a double labeling autoradiographic method. The total contribution of the spleen to erythropoiesis was also determined utilizing normal and splenectomized mice and evaluating the ensuing peripheral blood changes during hypoxia. Only the basophilic and the definitely erythroid spleen cell populations expanded markedly in response to hypoxia, reaching plateau levels during the first wk of exposure. During this time, the apparent DNA synthesis time [T(S)] and cell cycle time [T(C)] of the basophilic population remained unchanged, but a reduction of these parameters occurred in the erythroid population. T(s) fell from 7.2 to 5.5 h or less and T(C) decreased proportionately, since the labeling index remained unchanged. The authors concluded that the shortening of T(s) and T(C) in some spleen cell populations may be one of the mechanisms subserving the ultimate peripheral blood reticulocytosis and increased hematocrit. However, the spleen of C3H mice apparently functions only in an accessory capacity since the splenectomized animal appears to be capable of full compensation to the erythropoietic stress of prolonged hypoxia.

UR - http://www.scopus.com/inward/record.url?scp=0015743595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015743595&partnerID=8YFLogxK

M3 - Article

VL - 1

SP - 340

EP - 349

JO - Experimental Hematology

JF - Experimental Hematology

SN - 0301-472X

IS - 6

ER -