Kinetic analysis of splenic erythropoiesis in mice under prolonged hypoxic stress

A. M. Markoe, J. P. Okunewick, L. M. Schiffer

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The cytokinetics of C3H mouse spleen cells during compensatory erythropoiesis were evaluated by a double labeling autoradiographic method. The total contribution of the spleen to erythropoiesis was also determined utilizing normal and splenectomized mice and evaluating the ensuing peripheral blood changes during hypoxia. Only the basophilic and the definitely erythroid spleen cell populations expanded markedly in response to hypoxia, reaching plateau levels during the first wk of exposure. During this time, the apparent DNA synthesis time [T(S)] and cell cycle time [T(C)] of the basophilic population remained unchanged, but a reduction of these parameters occurred in the erythroid population. T(s) fell from 7.2 to 5.5 h or less and T(C) decreased proportionately, since the labeling index remained unchanged. The authors concluded that the shortening of T(s) and T(C) in some spleen cell populations may be one of the mechanisms subserving the ultimate peripheral blood reticulocytosis and increased hematocrit. However, the spleen of C3H mice apparently functions only in an accessory capacity since the splenectomized animal appears to be capable of full compensation to the erythropoietic stress of prolonged hypoxia.

Original languageEnglish (US)
Pages (from-to)340-349
Number of pages10
JournalExperimental Hematology
Issue number6
StatePublished - Dec 1 1973
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research


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