Kinetic analysis of nanoparticulate polyelectrolyte complex interactions with endothelial cells

Sean M. Hartig; Rachel R. Greene; Gianluca Carlesso; James N. Higginbotham; Wasif N. Khan; Ales Prokop; Jeffrey M. Davidson

doi:10.1016/j.biomaterials.2007.04.027

Kinetic analysis of nanoparticulate polyelectrolyte complex interactions with endothelial cells

Sean M. Hartig, Rachel R. Greene, Gianluca Carlesso, James N. Higginbotham, Wasif N. Khan, Ales Prokop, Jeffrey M. Davidson

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

A non-toxic, nanoparticulate polyelectrolyte complex (PEC) drug delivery system was formulated to maintain suitable physicochemical properties at physiological pH. Toxicity, binding, and internalization were evaluated in relevant microvascular endothelial cells. PEC were non-toxic, as indicated by cell proliferation studies and propidium iodide staining. Inhibitor studies revealed that PEC were bound, in part, via heparan sulfate proteoglycans and internalized through macropinocytosis. A novel, flow cytometric, Scatchard protocol was established and showed that PEC, in the absence of surface modification, bind cells non-specifically with positive cooperativity, as seen by graphical transformations.

Original language	English (US)
Pages (from-to)	3843-3855
Number of pages	13
Journal	Biomaterials
Volume	28
Issue number	26
DOIs	https://doi.org/10.1016/j.biomaterials.2007.04.027
State	Published - Sep 2007

Keywords

Drug delivery
Flow cytometry
Nanoparticles
Polyelectrolyte complexes
Scatchard plots

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering

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@article{bbc43fdfae9846d6b632d0078f8eac40,

title = "Kinetic analysis of nanoparticulate polyelectrolyte complex interactions with endothelial cells",

abstract = "A non-toxic, nanoparticulate polyelectrolyte complex (PEC) drug delivery system was formulated to maintain suitable physicochemical properties at physiological pH. Toxicity, binding, and internalization were evaluated in relevant microvascular endothelial cells. PEC were non-toxic, as indicated by cell proliferation studies and propidium iodide staining. Inhibitor studies revealed that PEC were bound, in part, via heparan sulfate proteoglycans and internalized through macropinocytosis. A novel, flow cytometric, Scatchard protocol was established and showed that PEC, in the absence of surface modification, bind cells non-specifically with positive cooperativity, as seen by graphical transformations.",

keywords = "Drug delivery, Flow cytometry, Nanoparticles, Polyelectrolyte complexes, Scatchard plots",

author = "Hartig, {Sean M.} and Greene, {Rachel R.} and Gianluca Carlesso and Higginbotham, {James N.} and Khan, {Wasif N.} and Ales Prokop and Davidson, {Jeffrey M.}",

note = "Funding Information: We acknowledge support of National Institutes of Health Grants 1R01EB002825 (J.M.D. and A.P) and 1RO1AI50213-01 (W.N.K), support from the Department of Veterans Affairs (J.M.D.), and American Cancer Society RSG 102299 (W.N.K.). In addition we would like to thank the Vanderbilt Institute for Nanoscale Science and Engineering (VINSE) for use of the Malvern ZetaSizer Nano ZS.",

year = "2007",

month = sep,

doi = "10.1016/j.biomaterials.2007.04.027",

language = "English (US)",

volume = "28",

pages = "3843--3855",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier BV",

number = "26",

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T1 - Kinetic analysis of nanoparticulate polyelectrolyte complex interactions with endothelial cells

AU - Hartig, Sean M.

AU - Greene, Rachel R.

AU - Carlesso, Gianluca

AU - Higginbotham, James N.

AU - Khan, Wasif N.

AU - Prokop, Ales

AU - Davidson, Jeffrey M.

N1 - Funding Information: We acknowledge support of National Institutes of Health Grants 1R01EB002825 (J.M.D. and A.P) and 1RO1AI50213-01 (W.N.K), support from the Department of Veterans Affairs (J.M.D.), and American Cancer Society RSG 102299 (W.N.K.). In addition we would like to thank the Vanderbilt Institute for Nanoscale Science and Engineering (VINSE) for use of the Malvern ZetaSizer Nano ZS.

PY - 2007/9

Y1 - 2007/9

N2 - A non-toxic, nanoparticulate polyelectrolyte complex (PEC) drug delivery system was formulated to maintain suitable physicochemical properties at physiological pH. Toxicity, binding, and internalization were evaluated in relevant microvascular endothelial cells. PEC were non-toxic, as indicated by cell proliferation studies and propidium iodide staining. Inhibitor studies revealed that PEC were bound, in part, via heparan sulfate proteoglycans and internalized through macropinocytosis. A novel, flow cytometric, Scatchard protocol was established and showed that PEC, in the absence of surface modification, bind cells non-specifically with positive cooperativity, as seen by graphical transformations.

AB - A non-toxic, nanoparticulate polyelectrolyte complex (PEC) drug delivery system was formulated to maintain suitable physicochemical properties at physiological pH. Toxicity, binding, and internalization were evaluated in relevant microvascular endothelial cells. PEC were non-toxic, as indicated by cell proliferation studies and propidium iodide staining. Inhibitor studies revealed that PEC were bound, in part, via heparan sulfate proteoglycans and internalized through macropinocytosis. A novel, flow cytometric, Scatchard protocol was established and showed that PEC, in the absence of surface modification, bind cells non-specifically with positive cooperativity, as seen by graphical transformations.

KW - Drug delivery

KW - Flow cytometry

KW - Nanoparticles

KW - Polyelectrolyte complexes

KW - Scatchard plots

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