Kinetic analysis of nanoparticulate polyelectrolyte complex interactions with endothelial cells

Sean M. Hartig, Rachel R. Greene, Gianluca Carlesso, James N. Higginbotham, Wasif N. Khan, Ales Prokop, Jeffrey M. Davidson

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

A non-toxic, nanoparticulate polyelectrolyte complex (PEC) drug delivery system was formulated to maintain suitable physicochemical properties at physiological pH. Toxicity, binding, and internalization were evaluated in relevant microvascular endothelial cells. PEC were non-toxic, as indicated by cell proliferation studies and propidium iodide staining. Inhibitor studies revealed that PEC were bound, in part, via heparan sulfate proteoglycans and internalized through macropinocytosis. A novel, flow cytometric, Scatchard protocol was established and showed that PEC, in the absence of surface modification, bind cells non-specifically with positive cooperativity, as seen by graphical transformations.

Original languageEnglish (US)
Pages (from-to)3843-3855
Number of pages13
JournalBiomaterials
Volume28
Issue number26
DOIs
StatePublished - Sep 1 2007
Externally publishedYes

Keywords

  • Drug delivery
  • Flow cytometry
  • Nanoparticles
  • Polyelectrolyte complexes
  • Scatchard plots

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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  • Cite this

    Hartig, S. M., Greene, R. R., Carlesso, G., Higginbotham, J. N., Khan, W. N., Prokop, A., & Davidson, J. M. (2007). Kinetic analysis of nanoparticulate polyelectrolyte complex interactions with endothelial cells. Biomaterials, 28(26), 3843-3855. https://doi.org/10.1016/j.biomaterials.2007.04.027