Keratinocyte growth factor protects epedermis and hair follicles from cell death induced by UV irradiation, chemotherapeutic or cytotoxic agents

Susanne Braun, Monika Krampert, Enikö Bodó, Angelika Kümin, Christiane Born-Berclaz, Ralf Paus, Sabine Werner

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Owing to its potent cytoprotective properties for epithelial cells, keratinocyte growth factor (KGF) is successfully used for the treatment of chemotherapy- and radiotherapy-induced oral mucositis in cancer patients. It is therefore of major interest to determine possible clinical applications of KGF in other organs and in different stress situations and to unravel common and organ-specific mechanisms of KGF action. Here we show that KGF protects human keratinocytes from the toxicity of xenobiotics with electrophilic and oxidative properties and reduces the cell death induced by UV irradiation. In contrast to other cell types, cytoprotection of keratinocytes by KGF is not a direct anti-apoptotic effect but requires de novo protein synthesis. The in vitro findings are clinically relevant because KGF protected keratinocytes in organ-cultured human scalp hair follicles from the toxicity of the xenobiotic menadione. Moreover, injection of KGF into murine back skin markedly reduced cell death in the epidermis after UVB irradiation. This activity is dependent on FGF receptor signaling because it was abrogated in transgenic mice expressing a dominant-negative FGF receptor mutant in keratinocytes. Taken together, our results encourage the use of KGF for skin protection from chemical and physical insults.

Original languageEnglish (US)
Pages (from-to)4841-4849
Number of pages9
JournalJournal of Cell Science
Volume119
Issue number23
DOIs
StatePublished - Dec 1 2006
Externally publishedYes

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Fibroblast Growth Factor 7
Hair Follicle
Cytotoxins
Cell Death
Keratinocytes
Fibroblast Growth Factor Receptors
Xenobiotics
Vitamin K 3
Skin
Stomatitis
Cytoprotection
Scalp
Epidermis
Transgenic Mice
Radiotherapy
Epithelial Cells
Drug Therapy
Injections

Keywords

  • FGF
  • Keratinocyte
  • Reactive oxygen species
  • Regenerative medicine
  • UV

ASJC Scopus subject areas

  • Cell Biology

Cite this

Keratinocyte growth factor protects epedermis and hair follicles from cell death induced by UV irradiation, chemotherapeutic or cytotoxic agents. / Braun, Susanne; Krampert, Monika; Bodó, Enikö; Kümin, Angelika; Born-Berclaz, Christiane; Paus, Ralf; Werner, Sabine.

In: Journal of Cell Science, Vol. 119, No. 23, 01.12.2006, p. 4841-4849.

Research output: Contribution to journalArticle

Braun, Susanne ; Krampert, Monika ; Bodó, Enikö ; Kümin, Angelika ; Born-Berclaz, Christiane ; Paus, Ralf ; Werner, Sabine. / Keratinocyte growth factor protects epedermis and hair follicles from cell death induced by UV irradiation, chemotherapeutic or cytotoxic agents. In: Journal of Cell Science. 2006 ; Vol. 119, No. 23. pp. 4841-4849.
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