Jun, Fos and Krox in the hippocampus after noxious stimulation: Simultaneous-input-dependent expression and nuclear speckling

Damien Pearse, Asim Mirza, John Leah

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Stimulation of sensory C-fibres produces extensive expression of the Fos, Jun and Krox families of inducible transcription factors (ITFs) in many nociceptive CNS areas [28]. In the hippocampus, however, c-Fos is only weakly induced by such stimulation, and expression of the other ITFs has not been studied. Here we examine the effects of single, repeated and simultaneous C-fibre inputs on ITF expressions in the rat hippocampus. A brief, strong electrical stimulation of sciatic nerve C-fibres induced little or no expression of c-Fos or Krox-20. In contrast, FosB was induced and continued to rise in all areas, whereas the basal expressions of c-Jun and Krox-24 were initially reduced but then returned during the subsequent 36 h. A weak noxious cutaneous stimulus applied to one hindpaw induced only weak expressions of the ITFs. However, if the sciatic stimulation was applied contralaterally and 6 h beforehand, this weak stimulus strongly induced Krox-24, but not other ITFs, i.e. there was a potentiation of Krox-24 expression. When these two stimuli were applied simultaneously a few c-Fos labelled cells did appear, and there was and an increased Krox-24 expression. There was also a strong potentiation of FosB and a strong reduction in c-Jun expression. This simultaneous stimulation was the only type of stimulation to induce expression of Krox-20. Also after simultaneous stimulation the majority of the nuclear labelling for FosB, but not of the other ITFs, had a speckled appearance. MK-801 blocked these changes in ITF expressions, but it could also cause the C-fibre stimulations to induce c-Fos and c-Jun in specific areas of the hippocampus. Thus C-fibre stimulation does affect transcription factor activity in the hippocampus; and the strong responses of some ITFs to simultaneous inputs points to their having a role as 'genetic coincidence detectors' in the hippocampus.

Original languageEnglish (US)
Pages (from-to)193-208
Number of pages16
JournalBrain Research
Volume894
Issue number2
DOIs
StatePublished - Mar 16 2001
Externally publishedYes

Keywords

  • C-fiber
  • Fos
  • Hippocampus
  • Jun
  • Krox
  • Noxious
  • Nuclear speckling
  • Potentiation
  • Transcription factor

ASJC Scopus subject areas

  • Neuroscience(all)

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