Abstract
Stimulation of sensory C-fibres produces extensive expression of the Fos, Jun and Krox families of inducible transcription factors (ITFs) in many nociceptive CNS areas [28]. In the hippocampus, however, c-Fos is only weakly induced by such stimulation, and expression of the other ITFs has not been studied. Here we examine the effects of single, repeated and simultaneous C-fibre inputs on ITF expressions in the rat hippocampus. A brief, strong electrical stimulation of sciatic nerve C-fibres induced little or no expression of c-Fos or Krox-20. In contrast, FosB was induced and continued to rise in all areas, whereas the basal expressions of c-Jun and Krox-24 were initially reduced but then returned during the subsequent 36 h. A weak noxious cutaneous stimulus applied to one hindpaw induced only weak expressions of the ITFs. However, if the sciatic stimulation was applied contralaterally and 6 h beforehand, this weak stimulus strongly induced Krox-24, but not other ITFs, i.e. there was a potentiation of Krox-24 expression. When these two stimuli were applied simultaneously a few c-Fos labelled cells did appear, and there was and an increased Krox-24 expression. There was also a strong potentiation of FosB and a strong reduction in c-Jun expression. This simultaneous stimulation was the only type of stimulation to induce expression of Krox-20. Also after simultaneous stimulation the majority of the nuclear labelling for FosB, but not of the other ITFs, had a speckled appearance. MK-801 blocked these changes in ITF expressions, but it could also cause the C-fibre stimulations to induce c-Fos and c-Jun in specific areas of the hippocampus. Thus C-fibre stimulation does affect transcription factor activity in the hippocampus; and the strong responses of some ITFs to simultaneous inputs points to their having a role as 'genetic coincidence detectors' in the hippocampus.
| Original language | English |
|---|---|
| Pages (from-to) | 193-208 |
| Number of pages | 16 |
| Journal | Brain Research |
| Volume | 894 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 16 2001 |
| Externally published | Yes |
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Keywords
- C-fiber
- Fos
- Hippocampus
- Jun
- Krox
- Noxious
- Nuclear speckling
- Potentiation
- Transcription factor
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Jun, Fos and Krox in the hippocampus after noxious stimulation : Simultaneous-input-dependent expression and nuclear speckling. / Pearse, Damien D; Mirza, Asim; Leah, John.
In: Brain Research, Vol. 894, No. 2, 16.03.2001, p. 193-208.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Jun, Fos and Krox in the hippocampus after noxious stimulation
T2 - Simultaneous-input-dependent expression and nuclear speckling
AU - Pearse, Damien D
AU - Mirza, Asim
AU - Leah, John
PY - 2001/3/16
Y1 - 2001/3/16
N2 - Stimulation of sensory C-fibres produces extensive expression of the Fos, Jun and Krox families of inducible transcription factors (ITFs) in many nociceptive CNS areas [28]. In the hippocampus, however, c-Fos is only weakly induced by such stimulation, and expression of the other ITFs has not been studied. Here we examine the effects of single, repeated and simultaneous C-fibre inputs on ITF expressions in the rat hippocampus. A brief, strong electrical stimulation of sciatic nerve C-fibres induced little or no expression of c-Fos or Krox-20. In contrast, FosB was induced and continued to rise in all areas, whereas the basal expressions of c-Jun and Krox-24 were initially reduced but then returned during the subsequent 36 h. A weak noxious cutaneous stimulus applied to one hindpaw induced only weak expressions of the ITFs. However, if the sciatic stimulation was applied contralaterally and 6 h beforehand, this weak stimulus strongly induced Krox-24, but not other ITFs, i.e. there was a potentiation of Krox-24 expression. When these two stimuli were applied simultaneously a few c-Fos labelled cells did appear, and there was and an increased Krox-24 expression. There was also a strong potentiation of FosB and a strong reduction in c-Jun expression. This simultaneous stimulation was the only type of stimulation to induce expression of Krox-20. Also after simultaneous stimulation the majority of the nuclear labelling for FosB, but not of the other ITFs, had a speckled appearance. MK-801 blocked these changes in ITF expressions, but it could also cause the C-fibre stimulations to induce c-Fos and c-Jun in specific areas of the hippocampus. Thus C-fibre stimulation does affect transcription factor activity in the hippocampus; and the strong responses of some ITFs to simultaneous inputs points to their having a role as 'genetic coincidence detectors' in the hippocampus.
AB - Stimulation of sensory C-fibres produces extensive expression of the Fos, Jun and Krox families of inducible transcription factors (ITFs) in many nociceptive CNS areas [28]. In the hippocampus, however, c-Fos is only weakly induced by such stimulation, and expression of the other ITFs has not been studied. Here we examine the effects of single, repeated and simultaneous C-fibre inputs on ITF expressions in the rat hippocampus. A brief, strong electrical stimulation of sciatic nerve C-fibres induced little or no expression of c-Fos or Krox-20. In contrast, FosB was induced and continued to rise in all areas, whereas the basal expressions of c-Jun and Krox-24 were initially reduced but then returned during the subsequent 36 h. A weak noxious cutaneous stimulus applied to one hindpaw induced only weak expressions of the ITFs. However, if the sciatic stimulation was applied contralaterally and 6 h beforehand, this weak stimulus strongly induced Krox-24, but not other ITFs, i.e. there was a potentiation of Krox-24 expression. When these two stimuli were applied simultaneously a few c-Fos labelled cells did appear, and there was and an increased Krox-24 expression. There was also a strong potentiation of FosB and a strong reduction in c-Jun expression. This simultaneous stimulation was the only type of stimulation to induce expression of Krox-20. Also after simultaneous stimulation the majority of the nuclear labelling for FosB, but not of the other ITFs, had a speckled appearance. MK-801 blocked these changes in ITF expressions, but it could also cause the C-fibre stimulations to induce c-Fos and c-Jun in specific areas of the hippocampus. Thus C-fibre stimulation does affect transcription factor activity in the hippocampus; and the strong responses of some ITFs to simultaneous inputs points to their having a role as 'genetic coincidence detectors' in the hippocampus.
KW - C-fiber
KW - Fos
KW - Hippocampus
KW - Jun
KW - Krox
KW - Noxious
KW - Nuclear speckling
KW - Potentiation
KW - Transcription factor
UR - http://www.scopus.com/inward/record.url?scp=0035896446&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035896446&partnerID=8YFLogxK
U2 - 10.1016/S0006-8993(01)01993-X
DO - 10.1016/S0006-8993(01)01993-X
M3 - Article
C2 - 11251193
AN - SCOPUS:0035896446
VL - 894
SP - 193
EP - 208
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 2
ER -