Isotopic ratio outlier analysis (IROA) of aqueous humor for metabolites

Maria Del Carmen Piqueras, Ciara Myer, Anna Junk, Sanjoy K. Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

“Omics” revolution during the last few decades has vastly increased the understanding of the biological processes that remained obscure in part due to the lack of quality data. Genomics, transcriptomics, epigenomics, proteomics, lipidomics, metabolomics, and microbiomics form an invaluable array of fields that contribute holistically to a global understanding of the intertwined roles of the basic constituents of life and how they comprehensively paint a picture of disease. Metabolomics, in particular, is the study of metabolites, which are the products of cellular metabolic processes (small molecules, i.e., fatty acids, amino acids, or carbohydrates, are part of metabolomics). In this chapter, it is explained how to achieve a metabolomics profiling of aqueous humor using a special isotopic carbon labeling, a novel technique that combines a tremendous effectiveness with simplicity. The very nature of this technique guarantees the exclusion of false positives, since the algorithms are based on the recognition of unique isotopic patterns.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages179-185
Number of pages7
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1996
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • HMDB
  • IROA
  • Isotopic labeling
  • Isotopic ratio
  • Mass spectrometry
  • Metabolites
  • Metabolomics
  • Omics
  • Orbitrap™

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Piqueras, M. D. C., Myer, C., Junk, A., & Bhattacharya, S. K. (2019). Isotopic ratio outlier analysis (IROA) of aqueous humor for metabolites. In Methods in Molecular Biology (pp. 179-185). (Methods in Molecular Biology; Vol. 1996). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9488-5_15