Abstract
Significant CRF activity was found in a fraction with R(f) = 0.82-0.7 or V(E)/V(T) = 0.41-0.48 obtained by gel filtration of acid extracts of pig hypothalami on Sephadex G-25. The activity of this fraction decreased markedly during subsequent purification, particularly in the last two steps. From this fraction, a heptapeptide with significant ACTH releasing activity in vitro, was isolated in pure state, and its amino acid sequence was established as H-Phe-Ile-Tyr-His-Ser-Tyr-Lys-OH. This heptapeptide was synthesized by solid phase methods. The CRF activity of synthetic heptapeptide in vitro was low but could be potentiated by a cofactor fraction from rat hypothalamic extract.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 228-232 |
| Number of pages | 5 |
| Journal | Unknown Journal |
| Volume | 13 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jan 1 1981 |
| Externally published | Yes |
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Biochemistry
- Endocrinology
- Clinical Biochemistry
- Biochemistry, medical
Fingerprint Dive into the research topics of 'Isolation, structure and synthesis of a heptapeptide with in vitro ACTH-releasing activity from porcine hypothalamus'. Together they form a unique fingerprint.
Cite this
Chang, R. C. C., Huang, W. Y., Arimura, A., Redding, T. W., Coy, D. H., Saffran, M., Kong, A., Hamilton, J. W., Cohn, D. V., & Schally, A. V. (1981). Isolation, structure and synthesis of a heptapeptide with in vitro ACTH-releasing activity from porcine hypothalamus. Unknown Journal, 13(4), 228-232. https://doi.org/10.1055/s-2007-1019228